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© The Rockefeller University Press, 0021-9525/1998//1501 $5.00
The Journal of Cell Biology, Volume 142, Number 6, , 1998 1501-1517

Assembly and Function of the Actin Cytoskeleton of Yeast: Relationships between Cables and Patches

Tatiana S. Karpova^*, James G. McNally, Samuel L. Moltz^*, and John A. Cooper^*

^* Department of Cell Biology and Physiology, Institute for Biomedical Computing, Washington University, St. Louis, Missouri 63110

Actin in eukaryotic cells is found in different pools, with filaments being organized into a variety of supramolecular assemblies. To investigate the assembly and functional relationships between different parts of the actin cytoskeleton in one cell, we studied the morphology and dynamics of cables and patches in yeast. The fine structure of actin cables and the manner in which cables disassemble support a model in which cables are composed of a number of overlapping actin filaments. No evidence for intrinsic polarity of cables was found.

To investigate to what extent different parts of the actin cytoskeleton depend on each other, we looked for relationships between cables and patches. Patches and cables were often associated, and their polarized distributions were highly correlated. Therefore, patches and cables do appear to depend on each other for assembly and function.

Many cell types show rearrangements of the actin cytoskeleton, which can occur via assembly or movement of actin filaments. In our studies, dramatic changes in actin polarization did not include changes in filamentous actin. In addition, the concentration of actin patches was relatively constant as cells grew. Therefore, cells do not have bursts of activity in which new parts of the actin cytoskeleton are created.

Key Words: actin • cytoskeleton • yeast • fluorescence microscopy • 3-D reconstructions

Abbreviations used in this paper: 2-D and 3-D, two- and three-dimensional; EM, maximum-likelihood estimation–maximization method; LLS, linear least-squares method; PSF, point-spread function; PWLS, penalized, weighted least-squares method; SEO, standard error of percentage; UB, SMB, and sLB, unbudded, small- to medium-budded, and large-budded.

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