Assembly and Function of the Actin Cytoskeleton of Yeast: Relationships between Cables and Patches

doi:10.1083/jcb.142.6.1501

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J. Cell Biol., Volume 142, Number 6, September 21, 1998 1501-1517

Assembly and Function of the Actin Cytoskeleton of Yeast: Relationships between Cables and Patches

Tatiana S. Karpova,^* James G. McNally, Samuel L. Moltz,^* and John A. Cooper^*

^* Department of Cell Biology and Physiology, Institute for Biomedical Computing, Washington University, St. Louis, Missouri 63110

Actin in eukaryotic cells is found in different pools, with filaments being organized into a variety of supramolecular assemblies.To investigate the assembly and functional relationships betweendifferent parts of the actin cytoskeleton in one cell, we studiedthe morphology and dynamics of cables and patches in yeast. Thefine structure of actin cables and the manner in which cablesdisassemble support a model in which cables are composed of anumber of overlapping actin filaments. No evidence for intrinsicpolarity of cables was found.

To investigate to what extent different parts of the actin cytoskeleton depend on each other, we looked for relationshipsbetween cables and patches. Patches and cables were often associated,and their polarized distributions were highly correlated. Therefore,patches and cables do appear to depend on each other for assemblyand function.

Many cell types show rearrangements of the actin cytoskeleton, which can occur via assembly or movement of actin filaments.In our studies, dramatic changes in actin polarization did notinclude changes in filamentous actin. In addition, the concentrationof actin patches was relatively constant as cells grew. Therefore,cells do not have bursts of activity in which new parts of theactin cytoskeleton are created.

Key words: actin; cytoskeleton; yeast; fluorescence microscopy; 3-D reconstructions

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