|
|
|
|
|
|
|
||
J. Cell Biol.,
Volume 143, Number 1, October 5, 1998 5-12
Department of Cell Biology, John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom
We have determined the relationship between overall nuclear architecture, chromosome territories, and transcription sites within the nucleus, using
three-dimensional confocal microscopy of well preserved tissue sections of wheat roots. Chromosome territories were visualized by GISH using rye genomic
probe in wheat/rye translocation and addition lines.
The chromosomes appeared as elongated regions and
showed a clear centromere-telomere polarization, with
the two visualized chromosomes lying approximately
parallel to one another across the nucleus. Labeling
with probes to telomeres and centromeres confirmed a
striking Rabl configuration in all cells, with a clear clustering of the centromeres, and cell files often maintained a common polarity through several division cycles. Transcription sites were detected by BrUTP
incorporation in unfixed tissue sections and revealed a
pattern of numerous foci uniformly distributed
throughout the nucleoplasm, as well as more intensely labeled foci in the nucleoli. It has been suggested that
the gene-rich regions in wheat chromosomes are clustered towards the telomeres. However, we found no indication of a difference in concentration of transcription sites between telomere and centromere poles of the nucleus. Neither could we detect any evidence that
the transcription sites were preferentially localized with
respect to the chromosome territorial boundaries.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
