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© The Rockefeller University Press, 0021-9525/1998//443 $5.00
The Journal of Cell Biology, Volume 143, Number 2, , 1998 443-455

Suppression of Radixin and Moesin Alters Growth Cone Morphology, Motility, and Process Formation In Primary Cultured Neurons

Gabriela Paglini^*, Patricia Kunda^*, Santiago Quiroga, Kenneth Kosik, and Alfredo Cáceres^*

^* Instituto Mercedes y Martin Ferreyra-CONICET, 5000 Cordoba, Argentina; Departamento Quimica Biologica (CIQUIBIC), Universidad Nacional Cordoba/CONICET, 5000 Cordoba, Argentina; and Department of Neurology (Neuroscience), Harvard Medical School, and Center for Neurological Diseases, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115

In this study we have examined the cellular functions of ERM proteins in developing neurons. The results obtained indicate that there is a high degree of spatial and temporal correlation between the expression and subcellular localization of radixin and moesin with the morphological development of neuritic growth cones. More importantly, we show that double suppression of radixin and moesin, but not of ezrin–radixin or ezrin–moesin, results in reduction of growth cone size, disappearance of radial striations, retraction of the growth cone lamellipodial veil, and disorganization of actin filaments that invade the central region of growth cones where they colocalize with microtubules. Neuritic tips from radixin–moesin suppressed neurons displayed high filopodial protrusive activity; however, its rate of advance is 8–10 times slower than the one of growth cones from control neurons. Radixin–moesin suppressed neurons have short neurites and failed to develop an axon-like neurite, a phenomenon that appears to be directly linked with the alterations in growth cone structure and motility. Taken collectively, our data suggest that by regulating key aspects of growth cone development and maintenance, radixin and moesin modulate neurite formation and the development of neuronal polarity.

Key Words: ERM proteins • growth cones • actin filaments • neurite formation • axonal elongation

Abbreviations used in this paper: DIC, differential interference contrast; ERM, ezrin, radixin, and moesin; VEC-DIC, video-enhanced differential interference contrast.

Address all correspondence to Alfredo Cáceres, Instituto Mercedes y Martín Ferreyra, Casilla de Correo 389, 5000 Córdoba Argentina. Tel.: 54-51-681465. Fax: 54-51-695163. E-mail: acaceres{at}immf.uncor.edu

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