Time-Lapse Microscopy Reveals Unique Roles for Kinesins during Anaphase in Budding Yeast

doi:10.1083/jcb.143.3.687

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J. Cell Biol., Volume 143, Number 3, November 2, 1998 687-694

Time-Lapse Microscopy Reveals Unique Roles for Kinesins during Anaphase in Budding Yeast

Aaron F. Straight,^* John W. Sedat, and Andrew W. Murray^*

^* Department of Physiology and Department of Biochemistry, School of Medicine, University of California San Francisco, San Francisco, California 94143

The mitotic spindle is a complex and dynamic structure. Genetic analysis in budding yeast has identified two sets of kinesin-likemotors, Cin8p and Kip1p, and Kar3p and Kip3p, that have overlappingfunctions in mitosis. We have studied the role of three of thesemotors by video microscopy of motor mutants whose microtubulesand centromeres were marked with green fluorescent protein. Despitetheir functional overlap, each motor mutant has a specific defectin mitosis: cin8 $Delta$ mutants lack the rapid phase of anaphase B,kip1 $Delta$ mutants show defects in the slow phase of anaphase B, andkip3 $Delta$ mutants prolong the duration of anaphase to the point atwhich the spindle becomes longer than the cell. The kip3 $Delta$ andkip1 $Delta$ mutants affect the duration of anaphase, but cin8 $Delta$ doesnot.

Key words: mitosis; kinesin; microtubule; anaphase; yeast

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