Time-Lapse Microscopy Reveals Unique Roles for Kinesins during Anaphase in Budding Yeast

doi:10.1083/jcb.143.3.687

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© The Rockefeller University Press, 0021-9525/1998//687 $5.00
The Journal of Cell Biology, Volume 143, Number 3, , 1998 687-694

Regular Articles

Time-Lapse Microscopy Reveals Unique Roles for Kinesins during Anaphase in Budding Yeast

Aaron F. Straight^*, John W. Sedat, and Andrew W. Murray^*

^* Department of Physiology and Department of Biochemistry, School of Medicine, University of California San Francisco, San Francisco, California 94143

The mitotic spindle is a complex and dynamic structure. Geneticanalysis in budding yeast has identified two sets of kinesin-likemotors, Cin8p and Kip1p, and Kar3p and Kip3p, that have overlapping functions in mitosis. We have studied the role of three ofthese motors by video microscopy of motor mutants whose microtubulesand centromeres were marked with green fluorescent protein.Despite their functional overlap, each motor mutant has a specificdefect in mitosis: cin8 ${Delta}$ mutants lack the rapid phase of anaphase B, kip1 ${Delta}$ mutants show defects in the slow phase of anaphaseB, and kip3 ${Delta}$ mutants prolong the duration of anaphase to thepoint at which the spindle becomes longer than the cell. Thekip3 ${Delta}$ and kip1 ${Delta}$ mutants affect the duration of anaphase, but cin8 ${Delta}$ does not.

Key Words: mitosis • kinesin • microtubule • anaphase • yeast

Address all correspondence to Dr. Aaron F. Straight at his presentaddress Department of Cell Biology, Harvard Medical School,240 Longwood Ave., Boston, MA 02115. Tel.: (617) 432-3728. Fax:(617) 432-3702. E-mail: aaron_straight{at}hms.harvard.edu

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