Time-Lapse Microscopy Reveals Unique Roles for Kinesins during Anaphase in Budding Yeast

doi:10.1083/jcb.143.3.687

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© The Rockefeller University Press, 0021-9525/1998//687 $5.00
The Journal of Cell Biology, Volume 143, Number 3, , 1998 687-694

Regular Articles

Time-Lapse Microscopy Reveals Unique Roles for Kinesins during Anaphase in Budding Yeast

Aaron F. Straight^*, John W. Sedat, and Andrew W. Murray^*

^* Department of Physiology and Department of Biochemistry, School of Medicine, University of California San Francisco, San Francisco, California 94143

The mitotic spindle is a complex and dynamic structure. Geneticanalysis in budding yeast has identified two sets of kinesin-likemotors, Cin8p and Kip1p, and Kar3p and Kip3p, that have overlapping functions in mitosis. We have studied the role of three ofthese motors by video microscopy of motor mutants whose microtubulesand centromeres were marked with green fluorescent protein.Despite their functional overlap, each motor mutant has a specificdefect in mitosis: cin8 ${Delta}$ mutants lack the rapid phase of anaphase B, kip1 ${Delta}$ mutants show defects in the slow phase of anaphaseB, and kip3 ${Delta}$ mutants prolong the duration of anaphase to thepoint at which the spindle becomes longer than the cell. Thekip3 ${Delta}$ and kip1 ${Delta}$ mutants affect the duration of anaphase, but cin8 ${Delta}$ does not.

Key Words: mitosis • kinesin • microtubule • anaphase • yeast

Address all correspondence to Dr. Aaron F. Straight at his presentaddress Department of Cell Biology, Harvard Medical School,240 Longwood Ave., Boston, MA 02115. Tel.: (617) 432-3728. Fax:(617) 432-3702. E-mail: aaron_straight{at}hms.harvard.edu

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Hildebrandt, E. R., Gheber, L., Kingsbury, T., Hoyt, M. A. (2006). Homotetrameric Form of Cin8p, a Saccharomyces cerevisiae Kinesin-5 Motor, Is Essential for Its in Vivo Function. J. Biol. Chem. 281: 26004-26013 [Abstract] [Full Text]
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Davis, L., Smith, G. R. (2005). Dynein Promotes Achiasmate Segregation in Schizosaccharomyces pombe. Genetics 170: 581-590 [Abstract] [Full Text]
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Davis, L., Smith, G. R. (2003). Nonrandom Homolog Segregation at Meiosis I in Schizosaccharomyces pombe Mutants Lacking Recombination. Genetics 163: 857-874 [Abstract] [Full Text]
Schuyler, S. C., Liu, J. Y., Pellman, D. (2003). The molecular function of Ase1p: evidence for a MAP-dependent midzone-specific spindle matrix. JCB 160: 517-528 [Abstract] [Full Text]
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Adames, N. R., Oberle, J. R., Cooper, J. A. (2001). The Surveillance Mechanism of the Spindle Position Checkpoint in Yeast. JCB 153: 159-168 [Abstract] [Full Text]
Pearson, C. G., Maddox, P. S., Salmon, E.D., Bloom, K. (2001). Budding Yeast Chromosome Structure and Dynamics during Mitosis. JCB 152: 1255-1266 [Abstract] [Full Text]
Cheeseman, I. M., Enquist-Newman, M., Muller-Reichert, T., Drubin, D. G., Barnes, G. (2001). Mitotic Spindle Integrity and Kinetochore Function Linked by the Duo1p/Dam1p Complex. JCB 152: 197-212 [Abstract] [Full Text]
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Segal, M., Bloom, K., Reed, S. I. (2000). Bud6 Directs Sequential Microtubule Interactions with the Bud Tip and Bud Neck during Spindle Morphogenesis in Saccharomyces cerevisiae. Mol. Biol. Cell 11: 3689-3702 [Abstract] [Full Text]
Bloecher, A., Tatchell, K. (2000). Dynamic Localization of Protein Phosphatase Type 1 in the Mitotic Cell Cycle of Saccharomyces cerevisiae. JCB 149: 125-140 [Abstract] [Full Text]
Hunter, A., Wordeman, L (2000). How motor proteins influence microtubule polymerization dynamics. J. Cell Sci. 113: 4379-4389 [Abstract]
Sharp, D. J., Brown, H. M., Kwon, M., Rogers, G. C., Holland, G., Scholey, J. M. (2000). Functional Coordination of Three Mitotic Motors in Drosophila Embryos. Mol. Biol. Cell 11: 241-253 [Abstract] [Full Text]
Cottingham, F. R., Gheber, L., Miller, D. L., Hoyt, M. A. (1999). Novel Roles for Saccharomyces cerevisiae Mitotic Spindle Motors. JCB 147: 335-350 [Abstract] [Full Text]
Gordon, D. M., Roof, D. M. (1999). The Kinesin-related Protein Kip1p of Saccharomyces cerevisiae Is Bipolar. J. Biol. Chem. 274: 28779-28786 [Abstract] [Full Text]
Jones, M. H., Bachant, J. B., Castillo, A. R., Giddings, T. H. Jr., Winey, M. (1999). Yeast Dam1p Is Required to Maintain Spindle Integrity during Mitosis and Interacts with the Mps1p Kinase. Mol. Biol. Cell 10: 2377-2391 [Abstract] [Full Text]
Hardwick, K. G., Li, R., Mistrot, C., Chen, R.-H., Dann, P., Rudner, A., Murray, A. W. (1999). Lesions in Many Different Spindle Components Activate the Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisiae. Genetics 152: 509-518 [Abstract] [Full Text]
Manning, B. D., Barrett, J. G., Wallace, J. A., Granok, H., Snyder, M. (1999). Differential Regulation of the Kar3p Kinesin-related Protein by Two Associated Proteins, Cik1p and Vik1p. JCB 144: 1219-1233 [Abstract] [Full Text]
Biggins, S., Severin, F. F., Bhalla, N., Sassoon, I., Hyman, A. A., Murray, A. W. (1999). The conserved protein kinase Ipl1 regulates microtubule binding to kinetochores in budding yeast. Genes Dev. 13: 532-544 [Abstract] [Full Text]
Sharp, D. J., McDonald, K. L., Brown, H. M., Matthies, H. J., Walczak, C., Vale, R. D., Mitchison, T. J., Scholey, J. M. (1999). The Bipolar Kinesin, KLP61F, Cross-links Microtubules within Interpolar Microtubule Bundles of Drosophila Embryonic Mitotic Spindles. JCB 144: 125-138 [Abstract] [Full Text]
Odde, D., Ma, L, Briggs, A., DeMarco, A, Kirschner, M. (1999). Microtubule bending and breaking in living fibroblast cells. J. Cell Sci. 112: 3283-3288 [Abstract]
Gordon, D. M., Roof, D. M. (2001). Degradation of the kinesin Kip1p at anaphase onset is mediated by the anaphase-promoting complex and Cdc20p. Proc. Natl. Acad. Sci. USA 98: 12515-12520 [Abstract] [Full Text]
Hoepfner, D., Schaerer, F., Brachat, A., Wach, A., Philippsen, P. (2002). Reorientation of Mispositioned Spindles in Short Astral Microtubule Mutant spc72Delta Is Dependent on Spindle Pole Body Outer Plaque and Kar3 Motor Protein. Mol. Biol. Cell 13: 1366-1380 [Abstract] [Full Text]