Role of the Yeast Gin4p Protein Kinase in Septin Assembly and the Relationship between Septin Assembly and Septin Function

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© The Rockefeller University Press, 0021-9525/1998//719 $5.00
The Journal of Cell Biology, Volume 143, Number 3, , 1998 719-736

Regular Articles

Role of the Yeast Gin4p Protein Kinase in Septin Assembly and the Relationship between Septin Assembly and Septin Function

Mark S. Longtine, Hanna Fares, and John R. Pringle

Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599

To identify septin-interacting proteins in Saccharomyces cerevisiae,we screened for mutations that are synthetically lethal witha cdc12 septin mutation. One of the genes identified was GIN4,which encodes a protein kinase related to Hsl1p/Nik1p and Ycl024Wpin S. cerevisiae and to Nim1p/Cdr1p and Cdr2p in Schizosaccharomycespombe. The Gin4p kinase domain displayed a two-hybrid interactionwith the COOH-terminal portion of the Cdc3p septin, and Gin4pcolocalized with the septins at the mother–bud neck.This localization depended on the septins and on the COOH-terminal(nonkinase) region of Gin4p, and overproduction of this COOH-terminalregion led to a loss of septin organization and associatedmorphogenetic defects. We detected no effect of deleting YCL024W,either alone or in combination with deletion of GIN4. Deletionof GIN4 was not lethal but led to a striking reorganizationof the septins accompanied by morphogenetic abnormalities anda defect in cell separation; however, remarkably, cytokinesisappeared to occur efficiently. Two other proteins that localizeto the neck in a septin-dependent manner showed similar reorganizationsand also appeared to remain largely functional. The septinorganization observed in gin4 ${Delta}$ vegetative cells resembles thatseen normally in cells responding to mating pheromone, and noGin4p was detected in association with the septins in such cells.The organization of the septins observed in gin4 ${Delta}$ cells and in cells responding to pheromone appears to support some aspectsof the model for septin organization suggested previously byField et al. (Field, C.M., O. Al-Awar, J. Rosenblatt, M.L. Wong,B. Alberts, and T.J. Mitchison. 1996. J. Cell Biol. 133:605–616).

Key Words: cytokinesis • Gin4p • protein kinase • Saccharomyces cerevisiae • septins

Abbreviations used in this paper: AD, activation domain; DBD, DNA-binding domain; DIC, differential interference contrast; 5-FOA, 5-fluoroorotic acid; GST, glutathione-S-transferase; 3HA, triple hemagglutinin epitope; ORF, open reading frame; SC, synthetic complete medium; SD, synthetic minimal medium.

H. Fares's present address is Department of Biochemistry, ColumbiaUniversity, New York, NY 10032.

Address all correspondence to J.R. Pringle, Department of Biology, CB#3280, Coker Hall, University of North Carolina, Chapel Hill,NC 27599-3280. Tel.: (919) 962-2293. Fax: (919) 962-0320. E-mail:jpringle{at}email.unc.edu

2. Fares, H., M.S. Longtine, and J.R. Pringle, manuscript submittedfor publication.

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