Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 232K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pardo, L. A. Articles by Stühmer, W. Search for Related Content PubMed PubMed Citation Articles by Pardo, L. A. Articles by Stühmer, W. Social Bookmarking                            What's this?

© The Rockefeller University Press, 0021-9525/1998//767 $5.00
The Journal of Cell Biology, Volume 143, Number 3, , 1998 767-775

Cell Cycle–related Changes in the Conducting Properties of r-eag K⁺ Channels

Max-Planck-Institut für experimentelle Medizin, D-37075 Göttingen, Germany

Release from arrest in G2 phase of the cell cycle causes profound changes in rat ether-à-go-go (r-eag) K⁺ channels heterologously expressed in Xenopus oocytes. The most evident consequence of the onset of maturation is the appearance of rectification in the r-eag current. The trigger for these changes is located downstream of the activation of mitosis-promoting factor (MPF). We demonstrate here that the rectification is due to a voltage-dependent block by intracellular Na⁺ ions. Manipulation of the intracellular Na⁺ concentration indicates that the site of Na⁺ block is located 45% into the electrical distance of the pore and is only present in oocytes undergoing maturation. Since the currents through excised patches from immature oocytes exhibited a fast rundown, we studied CHO-K1 cells permanently transfected with r-eag. These cells displayed currents with a variable degree of block by Na⁺ and variable permeability to Cs⁺. Partial synchronization of the cultures in G0/G1 or M phases of the cell cycle greatly reduced the variability. The combined data obtained from mammalian cells and oocytes strongly suggest that the permeability properties of r-eag K⁺ channels are modulated during cell cycle–related processes.

Key Words: cell cycle • CHO cells • electrophysiology • potassium channels • Xenopus laevis

Abbreviations used in this paper: BAPTA, 1,2-bis(2-aminophenoxy)ethaneN,N,N'N'-tetraacetic acid; HO, Hoechst 33342; IC₅₀, concentration required for 50% inhibition; I-V, current–voltage; MPF, mitosis-promoting factor; NFR, normal frog Ringer; r-eag, rat ether-à-go-go K⁺ channel.

Address all correspondence to Luis A. Pardo, Max-Planck-Institut für experimentelle Medizin, Hermann-Rein-Str. 3, D-37075 Göttingen, Germany. Tel.: 49-551-3899-643. Fax: 49-551-389-9644. E-mail: pardo{at}mail.mpiem.gwdg.de

CiteULike

Complore

Connotea

Del.icio.us

Digg

Facebook

Reddit

Technorati

Twitter

This article has been cited by other articles:

• Bai, X., Ma, J., Pan, Z., Song, Y.-H., Freyberg, S., Yan, Y., Vykoukal, D., Alt, E. (2007). Electrophysiological properties of human adipose tissue-derived stem cells. Am. J. Physiol. Cell Physiol. 293: C1539-C1550 [Abstract] [Full Text]  
• Gomez-Varela, D., Zwick-Wallasch, E., Knotgen, H., Sanchez, A., Hettmann, T., Ossipov, D., Weseloh, R., Contreras-Jurado, C., Rothe, M., Stuhmer, W., Pardo, L. A. (2007). Monoclonal Antibody Blockade of the Human Eag1 Potassium Channel Function Exerts Antitumor Activity. Cancer Res. 67: 7343-7349 [Abstract] [Full Text]  
• Gang, H., Zhang, S. (2006). Na+ Permeation and Block of hERG Potassium Channels. JGP 128: 55-71 [Abstract] [Full Text]  
• Zhang, S. (2006). Isolation and characterization of IKr in cardiac myocytes by Cs+ permeation. Am. J. Physiol. Heart Circ. Physiol. 290: H1038-H1049 [Abstract] [Full Text]  
• Pardo, L. A. (2004). Voltage-Gated Potassium Channels in Cell Proliferation. Physiology 19: 285-292 [Abstract] [Full Text]  
• Farias, L. M. B., Ocana, D. B., Diaz, L., Larrea, F., Avila-Chavez, E., Cadena, A., Hinojosa, L. M., Lara, G., Villanueva, L. A., Vargas, C., Hernandez-Gallegos, E., Camacho-Arroyo, I., Duenas-Gonzalez, A., Perez-Cardenas, E., Pardo, L. A., Morales, A., Taja-Chayeb, L., Escamilla, J., Sanchez-Pena, C., Camacho, J. (2004). Ether a go-go Potassium Channels as Human Cervical Cancer Markers. Cancer Res. 64: 6996-7001 [Abstract] [Full Text]  
• Garcia-Ferreiro, R. E., Kerschensteiner, D., Major, F., Monje, F., Stuhmer, W., Pardo, L. A. (2004). Mechanism of Block of hEag1 K+ Channels by Imipramine and Astemizole. JGP 124: 301-317 [Abstract] [Full Text]  
• Konig, S., Hinard, V., Arnaudeau, S., Holzer, N., Potter, G., Bader, C. R., Bernheim, L. (2004). Membrane Hyperpolarization Triggers Myogenin and Myocyte Enhancer Factor-2 Expression during Human Myoblast Differentiation. J. Biol. Chem. 279: 28187-28196 [Abstract] [Full Text]  
• Winston, N.J., Johnson, M.H., McConnell, J.M., Cook, D.I., Day, M.L. (2004). Expression and Role of the Ether-a-Go-Go-Related (MERG1A) Potassium-Channel Protein During Preimplantation Mouse Development. Biol. Reprod. 70: 1070-1079 [Abstract] [Full Text]  
• Crociani, O., Guasti, L., Balzi, M., Becchetti, A., Wanke, E., Olivotto, M., Wymore, R. S., Arcangeli, A. (2003). Cell Cycle-dependent Expression of HERG1 and HERG1B Isoforms in Tumor Cells. J. Biol. Chem. 278: 2947-2955 [Abstract] [Full Text]  
• Murata, H., Tajima, N., Nagashima, Y., Yao, M., Baba, M., Goto, M., Kawamoto, S., Yamamoto, I., Okuda, K., Kanno, H. (2002). Von Hippel-Lindau Tumor Suppressor Protein Transforms Human Neuroblastoma Cells into Functional Neuron-like Cells. Cancer Res. 62: 7004-7011 [Abstract] [Full Text]  
• Mullins, F. M., Stepanovic, S. Z., Desai, R. R., George, A. L. Jr., Balser, J. R. (2002). Extracellular Sodium Interacts with the HERG Channel at an Outer Pore Site. JGP 120: 517-537 [Abstract] [Full Text]  
• Smith, G. A. M., Tsui, H.-W., Newell, E. W., Jiang, X., Zhu, X.-P., Tsui, F. W. L., Schlichter, L. C. (2002). Functional Up-regulation of HERG K+ Channels in Neoplastic Hematopoietic Cells. J. Biol. Chem. 277: 18528-18534 [Abstract] [Full Text]  
• Ghiani, C. A., Yuan, X., Eisen, A. M., Knutson, P. L., DePinho, R. A., McBain, C. J., Gallo, V. (1999). Voltage-Activated K+ Channels and Membrane Depolarization Regulate Accumulation of the Cyclin-Dependent Kinase Inhibitors p27Kip1 and p21CIP1 in Glial Progenitor Cells. J. Neurosci. 19: 5380-5392 [Abstract] [Full Text]  
• Machaca, K., Haun, S. (2000). Store-operated Calcium Entry Inactivates at the Germinal Vesicle Breakdown Stage of Xenopus Meiosis. J. Biol. Chem. 275: 38710-38715 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents