Role of Xklp3, a Subunit of the Xenopus Kinesin II Heterotrimeric Complex, in Membrane Transport between the Endoplasmic Reticulum and the Golgi Apparatus

doi:10.1083/jcb.143.6.1559

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J. Cell Biol., Volume 143, Number 6, December 14, 1998 1559-1573

Role of Xklp3, a Subunit of the Xenopus Kinesin II Heterotrimeric Complex, in Membrane Transport between the Endoplasmic Reticulum and the Golgi Apparatus

Nathalie Le Bot,^* Claude Antony, Jamie White,^* Eric Karsenti,^* and Isabelle Vernos^*

^* Cell Biology and Biophysics Program, European Molecular Biological Laboratory, D-69117 Heidelberg, Germany; and Institut Curie, 75248 Paris Cedex 05, France

The function of the Golgi apparatus is to modify proteins and lipids synthesized in the ER and sort them to their final destination.The steady-state size and function of the Golgi apparatus is maintainedthrough the recycling of some components back to the ER. Severallines of evidence indicate that the spatial segregation betweenthe ER and the Golgi apparatus as well as trafficking betweenthese two compartments require both microtubules and motors. Wehave cloned and characterized a new Xenopus kinesin like protein,Xklp3, a subunit of the heterotrimeric Kinesin II. By immunofluorescenceit is found in the Golgi region. A more detailed analysis by EMshows that it is associated with a subset of membranes that containthe KDEL receptor and are localized between the ER and Golgi apparatus.An association of Xklp3 with the recycling compartment is furthersupported by a biochemical analysis and the behavior of Xklp3in BFA-treated cells. The function of Xklp3 was analyzed by transfectingcells with a dominant-negative form lacking the motor domain.In these cells, the normal delivery of newly synthesized proteinsto the Golgi apparatus is blocked. Taken together, these resultsindicate that Xklp3 is involved in the transport of tubular-vesicularelements between the ER and the Golgi apparatus.

Key words: Kinesin II; microtubules; ER; Golgi; IC

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