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J. Cell Biol.,
Volume 143, Number 6, December 14, 1998 1559-1573

* Cell Biology and Biophysics Program, European Molecular Biological Laboratory, D-69117 Heidelberg, Germany; and The function of the Golgi apparatus is to
modify proteins and lipids synthesized in the ER and
sort them to their final destination. The steady-state
size and function of the Golgi apparatus is maintained through the recycling of some components back to the
ER. Several lines of evidence indicate that the spatial
segregation between the ER and the Golgi apparatus as
well as trafficking between these two compartments require both microtubules and motors. We have cloned
and characterized a new Xenopus kinesin like protein, Xklp3, a subunit of the heterotrimeric Kinesin II. By
immunofluorescence it is found in the Golgi region. A
more detailed analysis by EM shows that it is associated
with a subset of membranes that contain the KDEL receptor and are localized between the ER and Golgi apparatus. An association of Xklp3 with the recycling
compartment is further supported by a biochemical
analysis and the behavior of Xklp3 in BFA-treated
cells. The function of Xklp3 was analyzed by transfecting cells with a dominant-negative form lacking the motor domain. In these cells, the normal delivery of newly
synthesized proteins to the Golgi apparatus is blocked.
Taken together, these results indicate that Xklp3 is involved in the transport of tubular-vesicular elements
between the ER and the Golgi apparatus.
Institut Curie, 75248 Paris Cedex 05, France
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