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J. Cell Biol.,
Volume 143, Number 7, December 28, 1998 1775-1787
* Institut de Biochimie et Génétique Cellulaires, Centre National de la Recherche Scientifique, Unité Propre de Recherche 9026, 33077 Bordeaux, Cedex, France; and The spindle checkpoint ensures proper chromosome segregation by delaying anaphase until all
chromosomes are correctly attached to the mitotic spindle. We investigated the role of the fission yeast bub1
gene in spindle checkpoint function and in unperturbed mitoses. We find that bub1+ is essential for the fission
yeast spindle checkpoint response to spindle damage
and to defects in centromere function. Activation of the
checkpoint results in the recruitment of Bub1 to centromeres and a delay in the completion of mitosis. We
show that Bub1 also has a crucial role in normal, unperturbed mitoses. Loss of bub1 function causes chromosomes to lag on the anaphase spindle and an increased
frequency of chromosome loss. Such genomic instability is even more dramatic in Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, EH9 3JR
United Kingdom
bub1 diploids, leading to
massive chromosome missegregation events and loss of
the diploid state, demonstrating that bub1+ function is
essential to maintain correct ploidy through mitosis. As
in larger eukaryotes, Bub1 is recruited to kinetochores
during the early stages of mitosis. However, unlike its
vertebrate counterpart, a pool of Bub1 remains centromere-associated at metaphase and even until telophase. We discuss the possibility of a role for the Bub1
kinase after the metaphase-anaphase transition.
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