A Conserved LIM Protein That Affects Muscular Adherens Junction Integrity and Mechanosensory Function in Caenorhabditis elegans

doi:10.1083/jcb.144.1.45

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© The Rockefeller University Press, 0021-9525/1999//45 $5.00
The Journal of Cell Biology, Volume 144, Number 1, , 1999 45-57

Article

A Conserved LIM Protein That Affects Muscular Adherens Junction Integrity and Mechanosensory Function in Caenorhabditis elegans

Oliver Hobert^*, Donald G. Moerman, Kathleen A. Clark, Mary C. Beckerle, and Gary Ruvkun^*

^* Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114; Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z4; and Department of Biology, University of Utah, Salt Lake City, Utah 84112

We describe here the molecular and functional characterizationof the Caenorhabditis elegans unc-97 gene, whose gene productconstitutes a novel component of muscular adherens junctions.UNC-97 and homologues from several other species define the PINCH family, a family of LIM proteins whose modular compositionof five LIM domains implicates them as potential adapter molecules.unc-97 expression is restricted to tissue types that attachto the hypodermis, specifically body wall muscles, vulval muscles,and mechanosensory neurons. In body wall muscles, the UNC-97protein colocalizes with the β-integrin PAT-3 to the focaladhesion-like attachment sites of muscles. Partial and completeloss-of-function studies demonstrate that UNC-97 affects thestructural integrity of the integrin containing muscle adherensjunctions and contributes to the mechanosensory functions oftouch neurons. The expression of a Drosophila homologue of unc-97 in two integrin containing cell types, muscles, andmuscle-attached epidermal cells, suggests that unc-97 functionin adherens junction assembly and stability has been conservedacross phylogeny. In addition to its localization to adherensjunctions UNC-97 can also be detected in the nucleus, suggestingmultiple functions for this LIM domain protein.

Key Words: LIM domains • UNC-97 • muscle development • adherens junction • touch neuron

Abbreviations used in this paper: EST, expressed sequence tag; GFP, green fluorescent protein; LIM, LIN-11, ISL-1, MEC-3; MEC, mechanosensory; PAT, paralyzed and arrested at twofold stage; RNAi, RNA interference; UNC, uncoordinated.

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