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J. Cell Biol.,
Volume 144, Number 1, January 11, 1999 99-112



* Department of Cell Biology, Sciences III, University of Geneva, CH-1211 Geneva 4, Switzerland; and CLIP-170 is a cytoplasmic linker protein that
localizes to plus ends of microtubules in vivo. In this
study, we have characterized the microtubule-binding
properties of CLIP-170, to understand the mechanism
of its plus end targeting. We show that the NH2-terminal microtubule-interacting domain of CLIP-170 alone
localizes to microtubule plus ends when transfected
into cells. Association of CLIP-170 with newly-formed
microtubules was observed in cells microinjected with
biotinylated tubulin, used as a tracer for growing microtubules. Using in vitro assays, association of CLIP-170 with recently polymerized tubulin is also seen. Cross-linking and sedimentation velocity experiments suggest
association of CLIP-170 with nonpolymerized tubulin.
We conclude from these experiments that the microtubule end targeting of CLIP-170 is closely linked to tubulin polymerization.
Laboratoire
d'Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France
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