Dynamic Localization of CLIP-170 to Microtubule Plus Ends Is Coupled to Microtubule Assembly

doi:10.1083/jcb.144.1.99

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© The Rockefeller University Press, 0021-9525/1999//99 $5.00
The Journal of Cell Biology, Volume 144, Number 1, , 1999 99-112

Article

Dynamic Localization of CLIP-170 to Microtubule Plus Ends Is Coupled to Microtubule Assembly

Georgios S. Diamantopoulos^*, Franck Perez^*, Holly V. Goodson^*, Gérard Batelier, Ronald Melki, Thomas E. Kreis^*^,, and Janet E. Rickard^*

^* Department of Cell Biology, Sciences III, University of Geneva, CH-1211 Geneva 4, Switzerland; and Laboratoire d'Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France

CLIP-170 is a cytoplasmic linker protein that localizes toplus ends of microtubules in vivo. In this study, we have characterizedthe microtubule-binding properties of CLIP-170, to understandthe mechanism of its plus end targeting. We show that the NH₂-terminalmicrotubule-interacting domain of CLIP-170 alone localizesto microtubule plus ends when transfected into cells. Associationof CLIP-170 with newly-formed microtubules was observed incells microinjected with biotinylated tubulin, used as a tracerfor growing microtubules. Using in vitro assays, associationof CLIP-170 with recently polymerized tubulin is also seen.Cross-linking and sedimentation velocity experiments suggest association of CLIP-170 with nonpolymerized tubulin. We concludefrom these experiments that the microtubule end targeting ofCLIP-170 is closely linked to tubulin polymerization.

Key Words: CLIP-170 • MAPs • microtubules • polymerization • targeting

Abbreviations used in this paper: bt, biotin; CLIP, cytoplasmic linker protein; GMPCPP, guanylyl-( ${alpha}$ ,β)-methylene-diphosphonate; GTP ${gamma}$ S, guanosine 5'-O-(3-thiotriphosphate) tetralithium salt; MAP, microtubule-associated protein; MBP, microtubule-binding protein; rh, rhodamine.

J.E. Rickard's present address is Department of Mental Health,University Medical School, Foresterhill, Aberdeen AB25 2ZD,Scotland, UK.

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