Home | Help | Feedback | Subscriptions | Archive | Search | Search Results

This Article Full Text Full Text (PDF, 581K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kircher, S. Articles by Harter, K. Search for Related Content PubMed PubMed Citation Articles by Kircher, S. Articles by Harter, K. Social Bookmarking                            What's this?

© The Rockefeller University Press, 0021-9525/1999//201 $5.00
The Journal of Cell Biology, Volume 144, Number 2, , 1999 201-211

Nuclear Import of the Parsley bZIP Transcription Factor CPRF2 Is Regulated by Phytochrome Photoreceptors

Institut für Biologie II/Botanik, Universität Freiburg, 79104 Freiburg, Germany

In plants, light perception by photoreceptors leads to differential expression of an enormous number of genes. An important step for differential gene expression is the regulation of transcription factor activities. To understand these processes in light signal transduction we analyzed the three well-known members of the common plant regulatory factor (CPRF) family from parsley (Petroselinum crispum). Here, we demonstrate that these CPRFs, which belong to the basic- region leucine-zipper (bZIP) domain-containing transcription factors, are differentially distributed within parsley cells, indicating different regulatory functions within the regulatory networks of the plant cell. In particular, we show by cell fractionation and immunolocalization approaches that CPRF2 is transported from the cytosol into the nucleus upon irradiation due to action of phytochrome photoreceptors. Two NH₂-terminal domains responsible for cytoplasmic localization of CPRF2 in the dark were characterized by deletion analysis using a set of CPRF2-green fluorescent protein (GFP) gene fusion constructs transiently expressed in parsley protoplasts. We suggest that light-induced nuclear import of CPRF2 is an essential step in phytochrome signal transduction.

Key Words: light regulation • phytochrome • bZIP transcription factors • nucleocytoplasmic partitioning • retention domain

Abbreviations used in this paper: aa, amino acid; bZIP, basic-region leucine-zipper domain; CHS, chalcon synthase; CKII, casein kinase II; CPRF, common plant regulatory factor; EMSSA, electrophoretic mobility supershift assay; GBF, G-box–binding factor; GFP, green fluorescent protein; NLS, nuclear localization sequence; P_fr, far-red light absorbing form of phytochrome; phy, phytochrome; P_r, red light-absorbing form of phytochrome; r, recombinant; RG9, long wavelength far-red light.

S. Kircher and F. Wellmer contributed equally to this work.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Facebook

Reddit

Technorati

Twitter

This article has been cited by other articles:

• Yu, X., Sayegh, R., Maymon, M., Warpeha, K., Klejnot, J., Yang, H., Huang, J., Lee, J., Kaufman, L., Lin, C. (2009). Formation of Nuclear Bodies of Arabidopsis CRY2 in Response to Blue Light Is Associated with Its Blue Light-Dependent Degradation. Plant Cell 21: 118-130 [Abstract] [Full Text]  
• Lee, Y., Lee, H.-S., Lee, J.-S., Kim, S.-K., Kim, S.-H. (2008). Hormone- and light-regulated nucleocytoplasmic transport in plants: current status. J Exp Bot 59: 3229-3245 [Abstract] [Full Text]  
• Min, M. K., Kim, S. J., Miao, Y., Shin, J., Jiang, L., Hwang, I. (2007). Overexpression of Arabidopsis AGD7 Causes Relocation of Golgi-Localized Proteins to the Endoplasmic Reticulum and Inhibits Protein Trafficking in Plant Cells. Plant Physiol. 143: 1601-1614 [Abstract] [Full Text]  
• Hiltbrunner, A., Tscheuschler, A., Viczian, A., Kunkel, T., Kircher, S., Schafer, E. (2006). FHY1 and FHL Act Together to Mediate Nuclear Accumulation of the Phytochrome A Photoreceptor. Plant Cell Physiol 47: 1023-1034 [Abstract] [Full Text]  
• Huang, E., Blagg, S. L., Keller, T., Katoh, M., Shaulsky, G., Thompson, C. R. L. (2006). bZIP transcription factor interactions regulate DIF responses in Dictyostelium. Development 133: 449-458 [Abstract] [Full Text]  
• Sawant, S. V., Kiran, K., Mehrotra, R., Chaturvedi, C. P., Ansari, S. A., Singh, P., Lodhi, N., Tuli, R. (2005). A variety of synergistic and antagonistic interactions mediated by cis-acting DNA motifs regulate gene expression in plant cells and modulate stability of the transcription complex formed on a basal promoter. J Exp Bot 56: 2345-2353 [Abstract] [Full Text]  
• Bensmihen, S., Giraudat, J., Parcy, F. (2005). Characterization of three homologous basic leucine zipper transcription factors (bZIP) of the ABI5 family during Arabidopsis thaliana embryo maturation. J Exp Bot 56: 597-603 [Abstract] [Full Text]  
• Dai, S., Zhang, Z., Chen, S., Beachy, R. N. (2004). RF2b, a rice bZIP transcription activator, interacts with RF2a and is involved in symptom development of rice tungro disease. Proc. Natl. Acad. Sci. USA 101: 687-692 [Abstract] [Full Text]  
• Hare, P. D., Moller, S. G., Huang, L.-F., Chua, N.-H. (2003). LAF3, a Novel Factor Required for Normal Phytochrome A Signaling. Plant Physiol. 133: 1592-1604 [Abstract] [Full Text]  
• Cakir, B., Agasse, A., Gaillard, C., Saumonneau, A., Delrot, S., Atanassova, R. (2003). A Grape ASR Protein Involved in Sugar and Abscisic Acid Signaling. Plant Cell 15: 2165-2180 [Abstract] [Full Text]  
• Kagaya, Y., Hobo, T., Murata, M., Ban, A., Hattori, T. (2002). Abscisic Acid-Induced Transcription Is Mediated by Phosphorylation of an Abscisic Acid Response Element Binding Factor, TRAB1. Plant Cell 14: 3177-3189 [Abstract] [Full Text]  
• Hamann, T., Benkova, E., Baurle, I., Kientz, M., Jurgens, G. (2002). The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROS-mediated embryo patterning. Genes Dev. 16: 1610-1615 [Abstract] [Full Text]  
• Sweere, U., Eichenberg, K., Lohrmann, J., Mira-Rodado, V., Baurle, I., Kudla, J., Nagy, F., Schafer, E., Harter, K. (2001). Interaction of the Response Regulator ARR4 with Phytochrome B in Modulating Red Light Signaling. Science 294: 1108-1111 [Abstract] [Full Text]  
• Hanson, M. R., Kohler, R. H. (2001). GFP imaging: methodology and application to investigate cellular compartmentation in plants. J Exp Bot 52: 529-539 [Abstract] [Full Text]  
• Kiessling, J., Kruse, S., Rensing, S. A., Harter, K., Decker, E. L., Reski, R. (2000). Visualization of a Cytoskeleton-like Ftsz Network in Chloroplasts. JCB 151: 945-950 [Abstract] [Full Text]  
• Hisada, A., Hanzawa, H., Weller, J. L., Nagatani, A., Reid, J. B., Furuya, M. (2000). Light-Induced Nuclear Translocation of Endogenous Pea Phytochrome A Visualized by Immunocytochemical Procedures. Plant Cell 12: 1063-1078 [Abstract] [Full Text]  
• Wellmer, F., Kircher, S., Rugner, A., Frohnmeyer, H., Schafer, E., Harter, K. (1999). Phosphorylation of the Parsley bZIP Transcription Factor CPRF2 Is Regulated by Light. J. Biol. Chem. 274: 29476-29482 [Abstract] [Full Text]  
• Hudson, M., Ringli, C., Boylan, M. T., Quail, P. H. (1999). The FAR1 locus encodes a novel nuclear protein specific to phytochrome A signaling. Genes Dev. 13: 2017-2027 [Abstract] [Full Text]  
• Kircher, S., Kozma-Bognar, L., Kim, L., Adam, E., Harter, K., Schäfer, E., Nagy, F. (1999). Light Quality–Dependent Nuclear Import of the Plant Photoreceptors Phytochrome A and B. Plant Cell 11: 1445-1456 [Abstract] [Full Text]  
• Wellmer, F., Schafer, E., Harter, K. (2001). The DNA Binding Properties of the Parsley bZIP Transcription Factor CPRF4a Are Regulated by Light. J. Biol. Chem. 276: 6274-6279 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Search Results