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J. Cell Biol.,
Volume 144, Number 2, January 25, 1999 339-349
Department of Biology, University of Konstanz, D-78457 Konstanz, Germany
The optic disk-directed growth of retinal
ganglion cell axons is markedly disturbed in the presence of polyclonal antineurolin antibodies, which
mildly affect fasciculation (Ott, H., M. Bastmeyer, and
C.A.O. Stuermer, 1998. J. Neurosci. 18:3363-3372).
New monoclonal antibodies (mAbs) against goldfish
neurolin, an immunoglobulin (Ig) superfamily cell adhesion/recognition molecule with five Ig domains, were
generated to assign function (guidance versus fasciculation) to specific Ig domains. By their ability or failure to
recognize Chinese hamster ovary cells expressing recombinant neurolin with deletions of defined Ig domains, mAbs were identified as being directed against
Ig domains 1, 2, or 3, respectively. Repeated intraocular
injections of a mAb against Ig domain 2 disturb the
disk-directed growth: axons grow in aberrant routes
and fail to reach the optic disk, but remain fasciculated. mAbs against Ig domains 1 and 3 disturb the formation
of tight fascicles.
mAb against Ig domain 2 significantly increases the
incidence of growth cone departure from the disk-oriented fascicle track, while mAbs against Ig domains 1 and 3 do not. This was demonstrated by time-lapse
videorecording of labeled growth cones.
Thus, Ig domain 2 of neurolin is apparently essential
for growth cone guidance towards the disk, presumably
by being part of a receptor (or complex) for an axon
guidance component.
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