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© The Rockefeller University Press, 0021-9525/1999//519 $5.00
The Journal of Cell Biology, Volume 144, Number 3, , 1999 519-532

Regular Article

{delta}-catenin, an Adhesive Junction–associated Protein Which Promotes Cell Scattering



Qun Lu*, Mercedes Paredes*, Miguel Medina*, Jianhua Zhou*, Robert Cavallo{ddagger}, Mark Peifer{ddagger}, Lisa Orecchio*, and Kenneth S. Kosik*

* Center for Neurologic Diseases, Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and {ddagger} Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3280

The classical adherens junction that holds epithelial cells together consists of a protein complex in which members of the cadherin family linked to various catenins are the principal components. {delta}-catenin is a mammalian brain protein in the Armadillo repeat superfamily with sequence similarity to the adherens junction protein p120ctn. We found that {delta}-catenin can be immunoprecipitated as a complex with other components of the adherens junction, including cadherin and β-catenin, from transfected cells and brain. The interaction with cadherin involves direct contact within the highly conserved juxtamembrane region of the COOH terminus, where p120ctn also binds. In developing mouse brain, staining with {delta}-catenin antibodies is prominent towards the apical boundary of the neuroepithelial cells in the ventricular zone. When transfected into Madin-Darby canine kidney (MDCK) epithelial cells {delta}-catenin colocalized with cadherin, p120ctn, and β-catenin. The Arm domain alone was sufficient for achieving localization and coimmunoprecipitation with cadherin. The ectopic expression of {delta}-catenin in MDCK cells altered their morphology, induced the elaboration of lamellipodia, interfered with monolayer formation, and increased scattering in response to hepatocyte growth factor treatment. We propose that {delta}-catenin can regulate adhesion molecules to implement the organization of large cellular arrays necessary for tissue morphogenesis.

Key Words: {delta}-catenin • armadillo • adhesive junctions • cell motility • neural development

Abbreviations used in this paper: APC, adenomatous polyposis coli; BrdU, 5-bromo-2'-deoxy-uridine; GFP, green fluorescent protein; HGF, hepatocyte growth factor; IP, immunoprecipitation; MF, {delta}-catenin–transfected MDCK.


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