© The Rockefeller University Press,
0021-9525/1999//589 $5.00
The Journal of Cell Biology, Volume 144, Number 4,
, 1999 589-601
Telomere Length Dynamics and Chromosomal Instability in Cells Derived from Telomerase Null Mice
M. Prakash Hande
,
Enrique Samper*,
Peter Lansdorp
,
, and
María A. Blasco*
* Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Campus Cantoblanco, Madrid E-28049, Spain;
Terry Fox Laboratory, British Columbia Cancer Research Center, Vancouver, British Columbia V5Z 1L3, Canada; and
Department of Medicine, University of British Columbia, Vancouver, British Columbia V6T 2B5, Canada
To study the effect of continued telomere shortening on chromosome stability, we have analyzed the telomere length of two individual chromosomes (chromosomes 2 and 11) in fibroblasts derived from wild-type mice and from mice lacking the mouse telomerase RNA (mTER) gene using quantitative fluorescence in situ hybridization. Telomere length at both chromosomes decreased with increasing generations of mTER–/– mice. At the 6th mouse generation, this telomere shortening resulted in significantly shorter chromosome 2 telomeres than the average telomere length of all chromosomes. Interestingly, the most frequent fusions found in mTER–/– cells were homologous fusions involving chromosome 2. Immortal cultures derived from the primary mTER–/– cells showed a dramatic accumulation of fusions and translocations, revealing that continued growth in the absence of telomerase is a potent inducer of chromosomal instability. Chromosomes 2 and 11 were frequently involved in these abnormalities suggesting that, in the absence of telomerase, chromosomal instability is determined in part by chromosome-specific telomere length. At various points during the growth of the immortal mTER–/– cells, telomere length was stabilized in a chromosome-specific man-ner. This telomere-maintenance in the absence of telomerase could provide the basis for the ability of mTER–/– cells to grow indefinitely and form tumors.
Key Words: telomerase-deficient mice telomeres chromosome fusions Q-FISH cancer
Abbreviations used in this paper: MEF, mouse embryonic fibroblast; mTER, mouse telomerase RNA gene; PD, population doubling; Q-FISH, quantitative fluorescence in situ hybridization; TFU, telomere fluorescence units; wt, wild-type.
Address correspondence to María A. Blasco, Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Campus Cantoblanco, Madrid E-28049, Spain. Tel.: 34 91 585 4846. Fax: 34 91 372 0493. E-mail: mblasco{at}cnb.uam.es

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