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© The Rockefeller University Press, 0021-9525/1999//1019 $5.00
The Journal of Cell Biology, Volume 144, Number 5, , 1999 1019-1031

Protein Tyrosine Phosphatase-PEST Regulates Focal Adhesion Disassembly, Migration, and Cytokinesis in Fibroblasts

Alexandre Angers-Loustau^*, Jean-François Côté^*, Alain Charest, Donald Dowbenko, Susan Spencer, Laurence A. Lasky, and Michel L. Tremblay^*

^* Department of Biochemistry, McGill University, Montréal, Québec, Canada H3G 1Y6; Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; and Department of Molecular Oncology, Genentech, Inc., South San Francisco, California 94080

In this article, we show that, in transfected COS-1 cells, protein tyrosine phosphatase (PTP)-PEST translocates to the membrane periphery following stimulation by the extracellular matrix protein fibronectin. When plated on fibronectin, PTP-PEST (–/–) fibroblasts display a strong defect in motility. 3 h after plating on fibronectin, the number and size of vinculin containing focal adhesions were greatly increased in the homozygous PTP-PEST mutant cells as compared with heterozygous cells. This phenomenon appears to be due in part to a constitutive increase in tyrosine phosphorylation of p130^CAS, a known PTP-PEST substrate, paxillin, which associates with PTP-PEST in vitro, and focal adhesion kinase (FAK). Another effect of this constitutive hyperphosphorylation, consistent with the focal adhesion regulation defect, is that (–/–) cells spread faster than the control cell line when plated on fibronectin. In the PTP-PEST (–/–) cells, an increase in affinity for the SH2 domains of Src and Crk towards p130^CAS was also observed. In (–/–) cells, we found a significant increase in the level of tyrosine phosphorylation of PSTPIP, a cleavage furrow–associated protein that interacts physically with all PEST family members. An effect of PSTPIP hyperphosphorylation appears to be that some cells remain attached at the site of the cleavage furrow for an extended period of time. In conclusion, our data suggest PTP-PEST plays a dual role in cell cytoskeleton organization, by promoting the turnover of focal adhesions required for cell migration, and by directly or indirectly regulating the proline, serine, threonine phosphatase interacting protein (PSTPIP) tyrosine phosphorylation level which may be involved in regulating cleavage furrow formation or disassembly during normal cell division.

Key Words: migration • cytokinesis • PTP-PEST • focal adhesion • PSTPIP

Abbreviations used in this paper: FAK, focal adhesion kinase; GST, glutathione S-transferase; HA, hemagglutinin antigen; PSTPIP, proline, serine, threonine phosphatase interacting protein; PTP, protein tyrosine phosphatase; SH, Src homology; WASP, Wiskott-Aldrich syndrome protein.

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