Control of Mitotic Spindle Position by the Saccharomyces cerevisiae Formin Bni1p

doi:10.1083/jcb.144.5.947

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© The Rockefeller University Press, 0021-9525/1999//947 $5.00
The Journal of Cell Biology, Volume 144, Number 5, , 1999 947-961

Regular Articles

Control of Mitotic Spindle Position by the Saccharomyces cerevisiae Formin Bni1p

Laifong Lee^*^,, Saskia K. Klee^*^,, Marie Evangelista^¶, Charles Boone^¶, and David Pellman^*

^* Department of Pediatric Oncology, The Dana-Farber Cancer Institute and Department of Pediatric Hematology, The Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544; Institut für Biochemie, Universität Stuttgart, 70569 Stuttgart, Germany; and ^¶ Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6

Alignment of the mitotic spindle with the axis of cell divisionis an essential process in Saccharomyces cerevisiae that ismediated by interactions between cytoplasmic microtubules andthe cell cortex. We found that a cortical protein, the yeastformin Bni1p, was required for spindle orientation. Two strikingabnormalities were observed in bni1 ${Delta}$ cells. First, the initialmovement of the spindle pole body (SPB) toward the emergingbud was defective. This phenotype is similar to that previouslyobserved in cells lacking the kinesin Kip3p and, in fact, BNI1and KIP3 were found to be in the same genetic pathway. Second,abnormal pulling interactions between microtubules and thecortex appeared to cause preanaphase spindles in bni1 ${Delta}$ cellsto transit back and forth between the mother and the bud. We therefore propose that Bni1p may localize or alter the functionof cortical microtubule-binding sites in the bud. Additionally,we present evidence that other bipolar bud site determinantstogether with cortical actin are also required for spindleorientation.

Key Words: mitotic spindle apparatus • cell division • microtubule • actin • Saccharomyces cerevisiae

Abbreviations used in this paper: centromere-based; DAPI, 4',6'-diamidino-2-phenylindole; DIC, differential interference contrast; GFP, green fluorescent protein; Lat-A, Latrunculin-A; SPB, spindle pole body.

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