Dynamics of Myoblast Transplantation Reveal a Discrete Minority of Precursors with Stem Cell-like Properties as the Myogenic Source

doi:10.1083/jcb.144.6.1113

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J. Cell Biol., Volume 144, Number 6, March 22, 1999 1113-1122

Dynamics of Myoblast Transplantation Reveal a Discrete Minority of Precursors with Stem Cell-like Properties as the Myogenic Source

Jonathan R. Beauchamp, Jennifer E. Morgan, Charles N. Pagel, and Terence A. Partridge

Muscle Cell Biology Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London W12 0NN, United Kingdom

Myoblasts, the precursors of skeletal muscle fibers, can be induced to withdraw from the cell cycle and differentiate in vitro.Recent studies have also identified undifferentiated subpopulationsthat can self-renew and generate myogenic cells (Baroffio, A.,M. Hamann, L. Bernheim, M.-L. Bochaton-Pillat, G. Gabbiani, andC.R. Bader. 1996. Differentiation. 60:47-57; Yoshida, N., S. Yoshida,K. Koishi, K. Masuda, and Y. Nabeshima. 1998. J. Cell Sci. 111:769-779).Cultured myoblasts can also differentiate and contribute to repairand new muscle formation in vivo, a capacity exploited in attemptsto develop myoblast transplantation (MT) for genetic modificationof adult muscle. Our studies of the dynamics of MT demonstratethat cultures of myoblasts contain distinct subpopulations definedby their behavior in vitro and divergent responses to grafting.By comparing a genomic and a semiconserved marker, we have followedthe fate of myoblasts transplanted into muscles of dystrophicmice, finding that the majority of the grafted cells quickly dieand only a minority are responsible for new muscle formation.This minority is behaviorally distinct, slowly dividing in tissueculture, but rapidly proliferative after grafting, suggestinga subpopulation with stem cell-likecharacteristics.

Key words: skeletal muscle; myoblast transplantation; cell heterogeneity; stem cells; mdx mouse

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