Dynamics of Myoblast Transplantation Reveal a Discrete Minority of Precursors with Stem Cell-like Properties as the Myogenic Source

doi:10.1083/jcb.144.6.1113

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© The Rockefeller University Press, 0021-9525/1999//1113 $5.00
The Journal of Cell Biology, Volume 144, Number 6, , 1999 1113-1122

Regular Articles

Dynamics of Myoblast Transplantation Reveal a Discrete Minority of Precursors with Stem Cell–like Properties as the Myogenic Source

Jonathan R. Beauchamp, Jennifer E. Morgan, Charles N. Pagel, and Terence A. Partridge

Muscle Cell Biology Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London W12 0NN, United Kingdom

Myoblasts, the precursors of skeletal muscle fibers, can beinduced to withdraw from the cell cycle and differentiate invitro. Recent studies have also identified undifferentiatedsubpopulations that can self-renew and generate myogenic cells(Baroffio, A., M. Hamann, L. Bernheim, M.-L. Bochaton-Pillat,G. Gabbiani, and C.R. Bader. 1996. Differentiation. 60:47–57; Yoshida, N., S. Yoshida, K. Koishi, K. Masuda, and Y. Nabeshima.1998. J. Cell Sci. 111:769–779). Cultured myoblasts canalso differentiate and contribute to repair and new muscle formationin vivo, a capacity exploited in attempts to develop myoblasttransplantation (MT) for genetic modification of adult muscle.Our studies of the dynamics of MT demonstrate that culturesof myoblasts contain distinct subpopulations defined by theirbehavior in vitro and divergent responses to grafting. By comparinga genomic and a semiconserved marker, we have followed the fateof myoblasts transplanted into muscles of dystrophic mice,finding that the majority of the grafted cells quickly dieand only a minority are responsible for new muscle formation.This minority is behaviorally distinct, slowly dividing in tissueculture, but rapidly proliferative after grafting, suggestinga subpopulation with stem cell–like characteristics.

Key Words: skeletal muscle • myoblast transplantation • cell heterogeneity • stem cells • mdx mouse

Abbreviations used in this paper: BrdU, 5-Bromo-2'-deoxyuridine; MT, myoblast transplantation; MPCs, muscle precursor cells; TA, tibialis anterior; TAg, SV-40 large T antigen.

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