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J. Cell Biol.,
Volume 145, Number 1, April 5, 1999 1-14
Department of Surgery, Department of Physiology, and Department of Molecular Medicine, and the MCO Microscopy Imaging
Center, Medical College of Ohio, Toledo, Ohio 43614
We used fluorescence in situ hybridization
(FISH) to study the positions of human chromosomes
on the mitotic rings of cultured human lymphocytes,
MRC-5 fibroblasts, and CCD-34Lu fibroblasts. The homologous chromosomes of all three cell types had relatively random positions with respect to each other
on the mitotic rings of prometaphase rosettes and
anaphase cells. Also, the positions of the X and Y chromosomes, colocalized with the somatic homologues in
male cells, were highly variable from one mitotic ring to
another. Although random chromosomal positions
were found in different pairs of CCD-34Lu and MRC-5
late-anaphases, the separations between the same homologous chromosomes in paired late-anaphase and
telophase chromosomal masses were highly correlated.
Thus, although some loose spatial associations of chromosomes secondary to interphase positioning may exist
on the mitotic rings of some cells, a fixed order of human chromosomes and/or a rigorous separation of homologous chromosomes on the mitotic ring are not necessary for normal mitosis. Furthermore, the relative
chromosomal positions on each individual metaphase
plate are most likely carried through anaphase into telophase.
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