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© The Rockefeller University Press, 0021-9525/1999//1 $5.00
The Journal of Cell Biology, Volume 145, Number 1, , 1999 1-14

Evidence for a Relatively Random Array of Human Chromosomes on the Mitotic Ring

Department of Surgery, Department of Physiology, and Department of Molecular Medicine, and the MCO Microscopy Imaging Center, Medical College of Ohio, Toledo, Ohio 43614

We used fluorescence in situ hybridization (FISH) to study the positions of human chromosomes on the mitotic rings of cultured human lymphocytes, MRC-5 fibroblasts, and CCD-34Lu fibroblasts. The homologous chromosomes of all three cell types had relatively random positions with respect to each other on the mitotic rings of prometaphase rosettes and anaphase cells. Also, the positions of the X and Y chromosomes, colocalized with the somatic homologues in male cells, were highly variable from one mitotic ring to another. Although random chromosomal positions were found in different pairs of CCD-34Lu and MRC-5 late-anaphases, the separations between the same homologous chromosomes in paired late-anaphase and telophase chromosomal masses were highly correlated. Thus, although some loose spatial associations of chromosomes secondary to interphase positioning may exist on the mitotic rings of some cells, a fixed order of human chromosomes and/or a rigorous separation of homologous chromosomes on the mitotic ring are not necessary for normal mitosis. Furthermore, the relative chromosomal positions on each individual metaphase plate are most likely carried through anaphase into telophase.

Key Words: chromosomal positions • mitotic ring • fluorescence in situ hybridization

Abbreviations used in this paper: FISH, fluorescence in situ hybridization; MRD, mitotic ring diameter.

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