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© The Rockefeller University Press, 0021-9525/1999//83 $5.00
The Journal of Cell Biology, Volume 145, Number 1, , 1999 83-98

GMAP-210, A Cis-Golgi Network-associated Protein, Is a Minus End Microtubule-binding Protein

Carlos Infante^*, Francisco Ramos-Morales^*, Concepción Fedriani^*, Michel Bornens, and Rosa M. Rios^*

^* Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla Apdo. 1095, 41080-Sevilla, Spain; and Institut Curie, Section Recherche, UMR144 du CNRS, 75248 Paris, Cedex 05, France

We report that a peripheral Golgi protein with a molecular mass of 210 kD localized at the cis-Golgi network (Rios, R.M., A.M. Tassin, C. Celati, C. Antony, M.C. Boissier, J.C. Homberg, and M. Bornens. 1994. J. Cell Biol. 125:997–1013) is a microtubule-binding protein that associates in situ with a subpopulation of stable microtubules. Interaction of this protein, now called GMAP-210, for Golgi microtubule-associated protein 210, with microtubules in vitro is direct, tight and nucleotide-independent. Biochemical analysis further suggests that GMAP-210 specifically binds to microtubule ends. The full-length cDNA encoding GMAP-210 predicts a protein of 1,979 amino acids with a very long central coiled-coil domain. Deletion analyses in vitro show that the COOH terminus of GMAP-210 binds to microtubules whereas the NH₂ terminus binds to Golgi membranes. Overexpression of GMAP-210–encoding cDNA induced a dramatic enlargement of the Golgi apparatus and perturbations in the microtubule network. These effects did not occur when a mutant lacking the COOH-terminal domain was expressed. When transfected in fusion with the green fluorescent protein, the NH₂-terminal domain associated with the cis-Golgi network whereas the COOH-terminal microtubule-binding domain localized at the centrosome. Altogether these data support the view that GMAP-210 serves to link the cis-Golgi network to the minus ends of centrosome-nucleated microtubules. In addition, this interaction appears essential for ensuring the proper morphology and size of the Golgi apparatus.

Key Words: autoantigens • Golgi apparatus • microtubule-associated proteins • microtubules • centrosome

Abbreviations used in this paper: AS, autoimmune serum; CGN, cis-Golgi network; CLIP, cytoplasmic linker protein; GFP, green fluorescent protein; GMAP, Golgi microtubule-associated protein; GST, glutathione-S-transferase; HSS, high speed supernatant; IB, immunoblotting; IF, immunofluorescence; NZ, nocodazole.

This paper is dedicated to the memory of Thomas Kreis.

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