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© The Rockefeller University Press, 0021-9525/1999//377 $5.00
The Journal of Cell Biology, Volume 145, Number 2, , 1999 377-389

Regular Articles

Dual Fatty Acylation of p59Fyn Is Required for Association with the T Cell Receptor {zeta} Chain through Phosphotyrosine–Src Homology Domain-2 Interactions



Woutervan't Hof and Marilyn D. Resh

Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York 10021

The first 10 residues within the Src homology domain (SH)–4 domain of the Src family kinase Fyn are required for binding to the immune receptor tyrosine-based activation motif (ITAM) of T cell receptor (TCR) subunits. Recently, mutation of glycine 2, cysteine 3, and lysines 7 and 9 was shown to block binding of Fyn to TCR {zeta} chain ITAMs, prompting the designation of these residues as an ITAM recognition motif (Gauen, L.K.T., M.E. Linder, and A.S. Shaw. 1996. J. Cell Biol. 133:1007–1015). Here we show that these residues do not mediate direct interactions with TCR ITAMs, but rather are required for efficient myristoylation and palmitoylation of Fyn. Specifically, coexpression of a K7,9A-Fyn mutant with N-myristoyltransferase restored myristoylation, membrane binding, and association with the cytoplasmic tail of TCR {zeta} fused to CD8. Conversely, treatment of cells with 2-hydroxymyristate, a myristoylation inhibitor, blocked association of wild-type Fyn with {zeta}. The Fyn NH2 terminus was necessary but not sufficient for interaction with {zeta} and both Fyn kinase and SH2 domains were required, directing phosphorylation of {zeta} ITAM tyrosines and binding to {zeta} ITAM phosphotyrosines. Fyn/{zeta} interaction was sensitive to octylglucoside and filipin, agents that disrupt membrane rafts. Moreover, a plasma membrane bound, farnesylated Fyn construct, G2A,C3S-FynKRas, was not enriched in the detergent insoluble fraction and did not associate with {zeta}. We conclude that the Fyn SH4 domain provides the signals for fatty acylation and specific plasma membrane localization, stabilizing the interactions between the Fyn SH2 domain and phosphotyrosines in TCR {zeta} chain ITAMs.

Key Words: Src homology domains • acylation • cell membrane • protein-tyrosine kinase • receptor/ antigen

Abbreviations used in this paper: GFP, green fluorescent protein; ITAM, immune-receptor tyrosine-based activation motif; NMT, N-myristoyl transferase; SH, Src homology domain; TCR, T cell receptor.

Dr. van't Hof's present address is Pulmonary Research Laboratories, Division of Pulmonary and Critical Care Medicine, Weill Medical College of Cornell University, 1300 York Ave., F-401, New York, NY 10021.


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