Paxillin LD4 Motif Binds PAK and PIX through a Novel 95-kD Ankyrin Repeat, ARF-GAP Protein: A Role in Cytoskeletal Remodeling

doi:10.1083/jcb.145.4.851

Quantitative Colocalization Analysis Software

A correction to this article has been published: J. Cell Biol. 145 (7) 1523

This Article

	Full Text
	Full Text (PDF, 1183K)
	PPT slides of all figures
	Correction (v145,p1523)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Turner, C. E.
	Articles by Leventhal, P. S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Turner, C. E.
	Articles by Leventhal, P. S.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/1999//851 $5.00
The Journal of Cell Biology, Volume 145, Number 4, , 1999 851-863

Regular Articles

Paxillin LD4 Motif Binds PAK and PIX through a Novel 95-kD Ankyrin Repeat, ARF–GAP Protein: A Role in Cytoskeletal Remodeling

Christopher E. Turner^*, Michael C. Brown^*, Joseph A. Perrotta^*, M.C. Riedy^*, Sotiris N. Nikolopoulos^*, A. Rosa McDonald^*, Shubha Bagrodia, Sheila Thomas, and Phillip S. Leventhal^*

^* Department of Anatomy and Cell Biology, State University of New York, Health Science Center, Syracuse, New York 13210; Department of Molecular Medicine, Cornell University, Ithaca, New York 14853; and Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215

Paxillin is a focal adhesion adaptor protein involved in theintegration of growth factor- and adhesion-mediated signal transductionpathways. Repeats of a leucine-rich sequence named paxillinLD motifs (Brown M.C., M.S. Curtis, and C.E. Turner. 1998.Nature Struct. Biol. 5:677–678) have been implicated in paxillin binding to focal adhesion kinase (FAK) and vinculin.Here we demonstrate that the individual paxillin LD motifs functionas discrete and selective protein binding interfaces. A novelscaffolding function is described for paxillin LD4 in the bindingof a complex of proteins containing active p21 GTPase–activatedkinase (PAK), Nck, and the guanine nucleotide exchange factor,PIX. The association of this complex with paxillin is mediatedby a new 95-kD protein, p95PKL (paxillin-kinase linker), whichbinds directly to paxillin LD4 and PIX. This protein complexalso binds to Hic-5, suggesting a conservation of LD functionacross the paxillin superfamily. Cloning of p95PKL revealeda multidomain protein containing an NH₂-terminal ARF–GAP domain, three ankyrin-like repeats, a potential calcium-bindingEF hand, calmodulin-binding IQ motifs, a myosin homology domain,and two paxillin-binding subdomains (PBS). Green fluorescentprotein- (GFP-) tagged p95PKL localized to focal adhesions/complexesin CHO.K1 cells. Overexpression in neuroblastoma cells ofa paxillin LD4 deletion mutant inhibited lamellipodia formationin response to insulin-like growth fac- tor-1. Microinjectionof GST–LD4 into NIH3T3 cells significantly decreasedcell migration into a wound. These data implicate paxillinas a mediator of p21 GTPase–regulated actin cytoskeletalreorganization through the recruitment to nascent focal adhesion structures of an active PAK/PIX complex potentially via interactionswith p95PKL.

Key Words: migration • signal transduction • integrins • trafficking • vesicular transport

Abbreviations used in this paper: aa, amino acid; FAK, focal adhesion kinase; FRNK, FAK-related nonkinase; GFP, green fluorescent protein; GIT, G protein-coupled receptor kinase-interacting protein; MLCK, myosin light chain kinase; PAK, p21 GTPase–activated kinase; PBS, paxillin binding subdomain; PIX, PAK-interacting exchange factor; PKL, paxillin-kinase linker.

CiteULike

Complore

Connotea

Del.icio.us Add to Digg

Digg

Facebook

Technorati

Twitter What's this?

This article has been cited by other articles:

Yu, J. A., Deakin, N. O., Turner, C. E. (2009). Paxillin-Kinase-Linker Tyrosine Phosphorylation Regulates Directional Cell Migration. Mol. Biol. Cell 20: 4706-4719 [Abstract] [Full Text]
Park, I.-W., He, J. J. (2009). HIV-1 Nef-mediated inhibition of T cell migration and its molecular determinants. J. Leukoc. Biol. 86: 1171-1178 [Abstract] [Full Text]
Hajdo-Milasinovic, A., van der Kammen, R. A., Moneva, Z., Collard, J. G. (2009). Rac3 inhibits adhesion and differentiation of neuronal cells by modifying GIT1 downstream signaling. J. Cell Sci. 122: 2127-2136 [Abstract] [Full Text]
Legate, K. R., Wickstrom, S. A., Fassler, R. (2009). Genetic and cell biological analysis of integrin outside-in signaling. Genes Dev. 23: 397-418 [Abstract] [Full Text]
Zouq, N. K., Keeble, J. A., Lindsay, J., Valentijn, A. J., Zhang, L., Mills, D., Turner, C. E., Streuli, C. H., Gilmore, A. P. (2009). FAK engages multiple pathways to maintain survival of fibroblasts and epithelia - differential roles for paxillin and p130Cas. J. Cell Sci. 122: 357-367 [Abstract] [Full Text]
Cavet, M. E., Pang, J., Yin, G., Berk, B. C. (2008). An epidermal growth factor (EGF) -dependent interaction between GIT1 and sorting nexin 6 promotes degradation of the EGF receptor. FASEB J. 22: 3607-3616 [Abstract] [Full Text]
Deakin, N. O., Turner, C. E. (2008). Paxillin comes of age. J. Cell Sci. 121: 2435-2444 [Abstract] [Full Text]
Zhang, Z. M., Simmerman, J. A., Guibao, C. D., Zheng, J. J. (2008). GIT1 Paxillin-binding Domain Is a Four-helix Bundle, and It Binds to Both Paxillin LD2 and LD4 Motifs. J. Biol. Chem. 283: 18685-18693 [Abstract] [Full Text]
Wade, R., Brimer, N., Vande Pol, S. (2008). Transformation by Bovine Papillomavirus Type 1 E6 Requires Paxillin. J. Virol. 82: 5962-5966 [Abstract] [Full Text]
Chahdi, A., Sorokin, A. (2008). Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt. Mol. Biol. Cell 19: 2609-2619 [Abstract] [Full Text]
Ha, V. L., Bharti, S., Inoue, H., Vass, W. C., Campa, F., Nie, Z., de Gramont, A., Ward, Y., Randazzo, P. A. (2008). ASAP3 Is a Focal Adhesion-associated Arf GAP That Functions in Cell Migration and Invasion. J. Biol. Chem. 283: 14915-14926 [Abstract] [Full Text]
Pang, J., Yan, C., Natarajan, K., Cavet, M. E., Massett, M. P., Yin, G., Berk, B. C. (2008). GIT1 Mediates HDAC5 Activation by Angiotensin II in Vascular Smooth Muscle Cells. Arterioscler. Thromb. Vasc. Bio. 28: 892-898 [Abstract] [Full Text]
Tang, D. D. (2008). Intermediate filaments in smooth muscle. Am. J. Physiol. Cell Physiol. 294: C869-C878 [Abstract] [Full Text]
Chahdi, A., Sorokin, A. (2008). Protein Kinase A-Dependent Phosphorylation Modulates {beta}1Pix Guanine Nucleotide Exchange Factor Activity through 14-3-3{beta} Binding. Mol. Cell. Biol. 28: 1679-1687 [Abstract] [Full Text]
Xie, Y., Tan, E-J., Wee, S., Manser, E., Lim, L., Koh, C.-G. (2008). Functional interactions between phosphatase POPX2 and mDia modulate RhoA pathways. J. Cell Sci. 121: 514-521 [Abstract] [Full Text]
McCARTHY, M. M. (2008). Estradiol and the Developing Brain. Physiol. Rev. 88: 91-134 [Abstract] [Full Text]
Totaro, A., Paris, S., Asperti, C., de Curtis, I. (2007). Identification of an Intramolecular Interaction Important for the Regulation of GIT1 Functions. Mol. Biol. Cell 18: 5124-5138 [Abstract] [Full Text]
Speert, D. B., Konkle, A. T. M., Zup, S. L., Schwarz, J. M., Shiroor, C., Taylor, M. E., McCarthy, M. M. (2007). Focal Adhesion Kinase and Paxillin: Novel Regulators of Brain Sexual Differentiation?. Endocrinology 148: 3391-3401 [Abstract] [Full Text]
Gerthoffer, W. T. (2007). Mechanisms of Vascular Smooth Muscle Cell Migration. Circ. Res. 100: 607-621 [Abstract] [Full Text]
Schober, M., Raghavan, S., Nikolova, M., Polak, L., Pasolli, H. A., Beggs, H. E., Reichardt, L. F., Fuchs, E. (2007). Focal adhesion kinase modulates tension signaling to control actin and focal adhesion dynamics. JCB 176: 667-680 [Abstract] [Full Text]
Hajdo-Milasinovic, A., Ellenbroek, S. I. J., van Es, S., van der Vaart, B., Collard, J. G. (2007). Rac1 and Rac3 have opposing functions in cell adhesion and differentiation of neuronal cells. J. Cell Sci. 120: 555-566 [Abstract] [Full Text]
Chang, F., Lemmon, C. A., Park, D., Romer, L. H. (2007). FAK Potentiates Rac1 Activation and Localization to Matrix Adhesion Sites: A Role for betaPIX. Mol. Biol. Cell 18: 253-264 [Abstract] [Full Text]
Li, Q.-F., Spinelli, A. M., Wang, R., Anfinogenova, Y., Singer, H. A., Tang, D. D. (2006). Critical Role of Vimentin Phosphorylation at Ser-56 by p21-activated Kinase in Vimentin Cytoskeleton Signaling. J. Biol. Chem. 281: 34716-34724 [Abstract] [Full Text]
Za, L., Albertinazzi, C., Paris, S., Gagliani, M., Tacchetti, C., de Curtis, I. (2006). {beta}PIX controls cell motility and neurite extension by regulating the distribution of GIT1. J. Cell Sci. 119: 2654-2666 [Abstract] [Full Text]
Boulter, E., Grall, D., Cagnol, S., Van Obberghen-Schilling, E. (2006). Regulation of cell-matrix adhesion dynamics and Rac-1 by integrin linked kinase. FASEB J. 20: 1489-1491 [Abstract] [Full Text]
Chahdi, A., Sorokin, A. (2006). Endothelin 1 Stimulates {beta}1Pix-Dependent Activation of Cdc42 Through the Gs{alpha} Pathway.. Exp. Biol. Med. 231: 761-765 [Abstract] [Full Text]
Hoefen, R. J., Berk, B. C. (2006). The multifunctional GIT family of proteins.. J. Cell Sci. 119: 1469-1475 [Abstract] [Full Text]
Vincent, P., Priceputu, E., Kay, D., Saksela, K., Jolicoeur, P., Hanna, Z. (2006). Activation of p21-activated Kinase 2 and Its Association with Nef Are Conserved in Murine Cells but Are Not Sufficient to Induce an AIDS-like Disease in CD4C/HIV Transgenic Mice. J. Biol. Chem. 281: 6940-6954 [Abstract] [Full Text]
Romer, L. H., Birukov, K. G., Garcia, J. G.N. (2006). Focal Adhesions: Paradigm for a Signaling Nexus. Circ. Res. 98: 606-616 [Abstract] [Full Text]
ten Klooster, J. P., Jaffer, Z. M., Chernoff, J., Hordijk, P. L. (2006). Targeting and activation of Rac1 are mediated by the exchange factor {beta}-Pix. JCB 172: 759-769 [Abstract] [Full Text]
Mehta, D., Malik, A. B. (2006). Signaling Mechanisms Regulating Endothelial Permeability. Physiol. Rev. 86: 279-367 [Abstract] [Full Text]
Jamieson, J. S., Tumbarello, D. A., Halle, M., Brown, M. C., Tremblay, M. L., Turner, C. E. (2005). Paxillin is essential for PTP-PEST-dependent regulation of cell spreading and motility: a role for paxillin kinase linker. J. Cell Sci. 118: 5835-5847 [Abstract] [Full Text]
Wu, R. F., Xu, Y. C., Ma, Z., Nwariaku, F. E., Sarosi, G. A. Jr., Terada, L. S. (2005). Subcellular targeting of oxidants during endothelial cell migration. JCB 171: 893-904 [Abstract] [Full Text]
Webb, D. J., Schroeder, M. J., Brame, C. J., Whitmore, L., Shabanowitz, J., Hunt, D. F., Horwitz, A. R. (2005). Paxillin phosphorylation sites mapped by mass spectrometry. J. Cell Sci. 118: 4925-4929 [Full Text]
Tumbarello, D. A., Brown, M. C., Hetey, S. E., Turner, C. E. (2005). Regulation of paxillin family members during epithelial-mesenchymal transformation: a putative role for paxillin {delta}. J. Cell Sci. 118: 4849-4863 [Abstract] [Full Text]
Webb, B. A., Eves, R., Crawley, S. W., Zhou, S., Cote, G. P., Mak, A. S. (2005). PAK1 induces podosome formation in A7r5 vascular smooth muscle cells in a PAK-interacting exchange factor-dependent manner. Am. J. Physiol. Cell Physiol. 289: C898-C907 [Abstract] [Full Text]
Liu, Y.-W., Huang, C.-F., Huang, K.-B., Lee, F.-J. S. (2005). Role for Gcs1p in Regulation of Arl1p at Trans-Golgi Compartments. Mol. Biol. Cell 16: 4024-4033 [Abstract] [Full Text]
Brown, M. C., Cary, L. A., Jamieson, J. S., Cooper, J. A., Turner, C. E. (2005). Src and FAK Kinases Cooperate to Phosphorylate Paxillin Kinase Linker, Stimulate Its Focal Adhesion Localization, and Regulate Cell Spreading and Protrusiveness. Mol. Biol. Cell 16: 4316-4328 [Abstract] [Full Text]
Yin, G., Zheng, Q., Yan, C., Berk, B. C. (2005). GIT1 Is a Scaffold for ERK1/2 Activation in Focal Adhesions. J. Biol. Chem. 280: 27705-27712 [Abstract] [Full Text]
Cau, J., Hall, A. (2005). Cdc42 controls the polarity of the actin and microtubule cytoskeletons through two distinct signal transduction pathways. J. Cell Sci. 118: 2579-2587 [Abstract] [Full Text]
Venkateswarlu, K., Hanada, T., Chishti, A. H. (2005). Centaurin-{alpha}1 interacts directly with kinesin motor protein KIF13B. J. Cell Sci. 118: 2471-2484 [Abstract] [Full Text]
Tanabe, K., Torii, T., Natsume, W., Braesch-Andersen, S., Watanabe, T., Satake, M. (2005). A Novel GTPase-activating Protein for ARF6 Directly Interacts with Clathrin and Regulates Clathrin-dependent Endocytosis. Mol. Biol. Cell 16: 1617-1628 [Abstract] [Full Text]
Liu, Y., Yerushalmi, G. M., Grigera, P. R., Parsons, J. T. (2005). Mislocalization or Reduced Expression of Arf GTPase-activating Protein ASAP1 Inhibits Cell Spreading and Migration by Influencing Arf1 GTPase Cycling. J. Biol. Chem. 280: 8884-8892 [Abstract] [Full Text]
Parsons, M., Monypenny, J., Ameer-Beg, S. M., Millard, T. H., Machesky, L. M., Peter, M., Keppler, M. D., Schiavo, G., Watson, R., Chernoff, J., Zicha, D., Vojnovic, B., Ng, T. (2005). Spatially Distinct Binding of Cdc42 to PAK1 and N-WASP in Breast Carcinoma Cells. Mol. Cell. Biol. 25: 1680-1695 [Abstract] [Full Text]
Rosenberger, G., Gal, A., Kutsche, K. (2005). {alpha}PIX Associates with Calpain 4, the Small Subunit of Calpain, and Has a Dual Role in Integrin-mediated Cell Spreading. J. Biol. Chem. 280: 6879-6889 [Abstract] [Full Text]
Chen, G.-C., Turano, B., Ruest, P. J., Hagel, M., Settleman, J., Thomas, S. M. (2005). Regulation of Rho and Rac Signaling to the Actin Cytoskeleton by Paxillin during Drosophila Development. Mol. Cell. Biol. 25: 979-987 [Abstract] [Full Text]
Chahdi, A., Miller, B., Sorokin, A. (2005). Endothelin 1 Induces {beta}1Pix Translocation and Cdc42 Activation via Protein Kinase A-dependent Pathway. J. Biol. Chem. 280: 578-584 [Abstract] [Full Text]
Yang, Z., Vadlamudi, R. K., Kumar, R. (2005). Dynein Light Chain 1 Phosphorylation Controls Macropinocytosis. J. Biol. Chem. 280: 654-659 [Abstract] [Full Text]
Chen, H.-Y., Shen, C.-H., Tsai, Y.-T., Lin, F.-C., Huang, Y.-P., Chen, R.-H. (2004). Brk Activates Rac1 and Promotes Cell Migration and Invasion by Phosphorylating Paxillin. Mol. Cell. Biol. 24: 10558-10572 [Abstract] [Full Text]
Hinsby, A. M., Olsen, J. V., Mann, M. (2004). Tyrosine Phosphoproteomics of Fibroblast Growth Factor Signaling: A ROLE FOR INSULIN RECEPTOR SUBSTRATE-4. J. Biol. Chem. 279: 46438-46447 [Abstract] [Full Text]
Valles, A. M., Beuvin, M., Boyer, B. (2004). Activation of Rac1 by Paxillin-Crk-DOCK180 Signaling Complex Is Antagonized by Rap1 in Migrating NBT-II Cells. J. Biol. Chem. 279: 44490-44496 [Abstract] [Full Text]
Mruk, D. D., Cheng, C. Y. (2004). Sertoli-Sertoli and Sertoli-Germ Cell Interactions and Their Significance in Germ Cell Movement in the Seminiferous Epithelium during Spermatogenesis. Endocr. Rev. 25: 747-806 [Abstract] [Full Text]
Brown, M. C., Turner, C. E. (2004). Paxillin: Adapting to Change. Physiol. Rev. 84: 1315-1339 [Abstract] [Full Text]
Zhang, Y., Chen, K., Tu, Y., Wu, C. (2004). Distinct Roles of Two Structurally Closely Related Focal Adhesion Proteins, {alpha}-Parvins and {beta}-Parvins, in Regulation of Cell Morphology and Survival. J. Biol. Chem. 279: 41695-41705 [Abstract] [Full Text]
Clarke, D. M., Brown, M. C., LaLonde, D. P., Turner, C. E. (2004). Phosphorylation of actopaxin regulates cell spreading and migration. JCB 166: 901-912 [Abstract] [Full Text]
Vindis, C., Teli, T., Cerretti, D. P., Turner, C. E., Huynh-Do, U. (2004). EphB1-mediated Cell Migration Requires the Phosphorylation of Paxillin at Tyr-31/Tyr-118. J. Biol. Chem. 279: 27965-27970 [Abstract] [Full Text]
Stofega, M. R., Sanders, L. C., Gardiner, E. M., Bokoch, G. M. (2004). Constitutive p21-activated Kinase (PAK) Activation in Breast Cancer Cells as a Result of Mislocalization of PAK to Focal Adhesions. Mol. Biol. Cell 15: 2965-2977 [Abstract] [Full Text]
Subauste, M. C., Pertz, O., Adamson, E. D., Turner, C. E., Junger, S., Hahn, K. M. (2004). Vinculin modulation of paxillin-FAK interactions regulates ERK to control survival and motility. JCB 165: 371-381 [Abstract] [Full Text]
Loo, T.-H., Ng, Y.-W., Lim, L., Manser, E. (2004). GIT1 Activates p21-Activated Kinase through a Mechanism Independent of p21 Binding. Mol. Cell. Biol. 24: 3849-3859 [Abstract] [Full Text]
van Nieuw Amerongen, G. P., Natarajan, K., Yin, G., Hoefen, R. J., Osawa, M., Haendeler, J., Ridley, A.J., Fujiwara, K., van Hinsbergh, V. W.M., Berk, B. C. (2004). GIT1 Mediates Thrombin Signaling in Endothelial Cells: Role in Turnover of RhoA-Type Focal Adhesions. Circ. Res. 94: 1041-1049 [Abstract] [Full Text]
Mishima, W., Suzuki, A., Yamaji, S., Yoshimi, R., Ueda, A., Kaneko, T., Tanaka, J., Miwa, Y., Ohno, S., Ishigatsubo, Y. (2004). The first CH domain of affixin activates Cdc42 and Rac1 through {alpha}PIX, a Cdc42/Rac1-specific guanine nucleotide exchanging factor. GENES CELLS 9: 193-204 [Abstract] [Full Text]
Ke, Y., Wang, L., Pyle, W. G., de Tombe, P. P., Solaro, R. J. (2004). Intracellular Localization and Functional Effects of P21-Activated Kinase-1 (Pak1) in Cardiac Myocytes. Circ. Res. 94: 194-200 [Abstract] [Full Text]
Shin, E.-Y., Woo, K.-N., Lee, C.-S., Koo, S.-H., Kim, Y. G., Kim, W.-J., Bae, C.-D., Chang, S.-I., Kim, E.-G. (2004). Basic Fibroblast Growth Factor Stimulates Activation of Rac1 through a p85 {beta}PIX Phosphorylation-dependent Pathway. J. Biol. Chem. 279: 1994-2004 [Abstract] [Full Text]
Yin, G., Haendeler, J., Yan, C., Berk, B. C. (2004). GIT1 Functions as a Scaffold for MEK1-Extracellular Signal-Regulated Kinase 1 and 2 Activation by Angiotensin II and Epidermal Growth Factor. Mol. Cell. Biol. 24: 875-885 [Abstract] [Full Text]
Haendeler, J., Yin, G., Hojo, Y., Saito, Y., Melaragno, M., Yan, C., Sharma, V. K., Heller, M., Aebersold, R., Berk, B. C. (2003). GIT1 Mediates Src-dependent Activation of Phospholipase C{gamma} by Angiotensin II and Epidermal Growth Factor. J. Biol. Chem. 278: 49936-49944 [Abstract] [Full Text]
Tang, D. D, Turner, C. E, Gunst, S. J (2003). Expression of non-phosphorylatable paxillin mutants in canine tracheal smooth muscle inhibits tension development. J. Physiol. 553: 21-35 [Abstract] [Full Text]
Goldfinger, L. E., Han, J., Kiosses, W. B., Howe, A. K., Ginsberg, M. H. (2003). Spatial restriction of {alpha}4 integrin phosphorylation regulates lamellipodial stability and {alpha}4{beta}1-dependent cell migration. JCB 162: 731-741 [Abstract] [Full Text]
Lamorte, L., Rodrigues, S., Sangwan, V., Turner, C. E., Park, M. (2003). Crk Associates with a Multimolecular Paxillin/GIT2/{beta}-PIX Complex and Promotes Rac-dependent Relocalization of Paxillin to Focal Contacts. Mol. Biol. Cell 14: 2818-2831 [Abstract] [Full Text]
DeMali, K. A., Burridge, K. (2003). Coupling membrane protrusion and cell adhesion. J. Cell Sci. 116: 2389-2397 [Abstract] [Full Text]
Fenster, S. D., Kessels, M. M., Qualmann, B., Chung, W. J., Nash, J., Gundelfinger, E. D., Garner, C. C. (2003). Interactions between Piccolo and the Actin/Dynamin-binding Protein Abp1 Link Vesicle Endocytosis to Presynaptic Active Zones. J. Biol. Chem. 278: 20268-20277 [Abstract] [Full Text]
Parsons, J. T. (2003). Focal adhesion kinase: the first ten years. J. Cell Sci. 116: 1409-1416 [Abstract] [Full Text]
Albertinazzi, C., Za, L., Paris, S., de Curtis, I. (2003). ADP-Ribosylation Factor 6 and a Functional PIX/p95-APP1 Complex Are Required for Rac1B-mediated Neurite Outgrowth. Mol. Biol. Cell 14: 1295-1307 [Abstract] [Full Text]
Shin, H., Wyszynski, M., Huh, K.-H., Valtschanoff, J. G., Lee, J.-R., Ko, J., Streuli, M., Weinberg, R. J., Sheng, M., Kim, E. (2003). Association of the Kinesin Motor KIF1A with the Multimodular Protein Liprin-alpha. J. Biol. Chem. 278: 11393-11401 [Abstract] [Full Text]
Gismondi, A., Jacobelli, J., Strippoli, R., Mainiero, F., Soriani, A., Cifaldi, L., Piccoli, M., Frati, L., Santoni, A. (2003). Proline-Rich Tyrosine Kinase 2 and Rac Activation by Chemokine and Integrin Receptors Controls NK Cell Transendothelial Migration. J. Immunol. 170: 3065-3073 [Abstract] [Full Text]
Ko, J., Kim, S., Valtschanoff, J. G., Shin, H., Lee, J.-R., Sheng, M., Premont, R. T., Weinberg, R. J., Kim, E. (2003). Interaction between Liprin-alpha and GIT1 Is Required for AMPA Receptor Targeting. J. Neurosci. 23: 1667-1677 [Abstract] [Full Text]
Kim, S., Ko, J., Shin, H., Lee, J.-R., Lim, C., Han, J.-H., Altrock, W. D., Garner, C. C., Gundelfinger, E. D., Premont, R. T., Kaang, B.-K., Kim, E. (2003). The GIT Family of Proteins Forms Multimers and Associates with the Presynaptic Cytomatrix Protein Piccolo. J. Biol. Chem. 278: 6291-6300 [Abstract] [Full Text]
Rosenberger, G., Jantke, I., Gal, A., Kutsche, K. (2003). Interaction of {alpha}PIX (ARHGEF6) with {beta}-parvin (PARVB) suggests an involvement of {alpha}PIX in integrin-mediated signaling. Hum Mol Genet 12: 155-167 [Abstract] [Full Text]
Vidal, C., Geny, B., Melle, J., Jandrot-Perrus, M., Fontenay-Roupie, M. (2002). Cdc42/Rac1-dependent activation of the p21-activated kinase (PAK) regulates human platelet lamellipodia spreading: implication of the cortical-actin binding protein cortactin. Blood 100: 4462-4469 [Abstract] [Full Text]
Nie, Z., Stanley, K. T., Stauffer, S., Jacques, K. M., Hirsch, D. S., Takei, J., Randazzo, P. A. (2002). AGAP1, an Endosome-associated, Phosphoinositide-dependent ADP-ribosylation Factor GTPase-activating Protein That Affects Actin Cytoskeleton. J. Biol. Chem. 277: 48965-48975 [Abstract] [Full Text]
Sastry, S. K., Lyons, P. D., Schaller, M. D., Burridge, K. (2002). PTP-PEST controls motility through regulation of Rac1. J. Cell Sci. 115: 4305-4316 [Abstract] [Full Text]
Garcia Arguinzonis, M. I., Galler, A. B., Walter, U., Reinhard, M., Simm, A. (2002). Increased Spreading, Rac/p21-activated Kinase (PAK) Activity, and Compromised Cell Motility in Cells Deficient in Vasodilator-stimulated Phosphoprotein (VASP). J. Biol. Chem. 277: 45604-45610 [Abstract] [Full Text]
Shin, E.-Y., Shin, K.-S., Lee, C.-S., Woo, K.-N., Quan, S.-H., Soung, N.-K., Kim, Y. G., Cha, C. I., Kim, S.-R., Park, D., Bokoch, G. M., Kim, E.-G. (2002). Phosphorylation of p85 beta PIX, a Rac/Cdc42-specific Guanine Nucleotide Exchange Factor, via the Ras/ERK/PAK2 Pathway Is Required for Basic Fibroblast Growth Factor-induced Neurite Outgrowth. J. Biol. Chem. 277: 44417-44430 [Abstract] [Full Text]
Wells, C. M., Abo, A., Ridley, A. J. (2002). PAK4 is activated via PI3K in HGF-stimulated epithelial cells. J. Cell Sci. 115: 3947-3956 [Abstract] [Full Text]
Pinco, K. A., He, W., Yang, J. T. (2002). alpha 4beta 1 Integrin Regulates Lamellipodia Protrusion via a Focal Complex/Focal Adhesion-independent Mechanism. Mol. Biol. Cell 13: 3203-3217 [Abstract] [Full Text]
Tang, D. D, Wu, M.-F., Opazo Saez, A. M, Gunst, S. J (2002). The focal adhesion protein paxillin regulates contraction in canine tracheal smooth muscle. J. Physiol. 542: 501-513 [Abstract] [Full Text]
Liu, Y., Loijens, J. C., Martin, K. H., Karginov, A. V., Parsons, J. T. (2002). The Association of ASAP1, an ADP Ribosylation Factor-GTPase Activating Protein, with Focal Adhesion Kinase Contributes to the Process of Focal Adhesion Assembly. Mol. Biol. Cell 13: 2147-2156 [Abstract] [Full Text]
Liu, S., Kiosses, W. B., Rose, D. M., Slepak, M., Salgia, R., Griffin, J. D., Turner, C. E., Schwartz, M. A., Ginsberg, M. H. (2002). A Fragment of Paxillin Binds the alpha 4 Integrin Cytoplasmic Domain (Tail) and Selectively Inhibits alpha 4-Mediated Cell Migration. J. Biol. Chem. 277: 20887-20894 [Abstract] [Full Text]
Brown, M. C., West, K. A., Turner, C. E. (2002). Paxillin-dependent Paxillin Kinase Linker and p21-Activated Kinase Localization to Focal Adhesions Involves a Multistep Activation Pathway. Mol. Biol. Cell 13: 1550-1565 [Abstract] [Full Text]
Chay, K.-O., Park, S. S., Mushinski, J. F. (2002). Linkage of Caspase-mediated Degradation of Paxillin to Apoptosis in Ba/F3 Murine Pro-B Lymphocytes. J. Biol. Chem. 277: 14521-14529 [Abstract] [Full Text]
Matafora, V., Paris, S., Dariozzi, S., Curtis, I. d. (2002). Molecular mechanisms regulating the subcellular localization of p95-APP1 between the endosomal recycling compartment and sites of actin organization at the cell surface. J. Cell Sci. 114: 4509-4520 [Abstract] [Full Text]
Woods, A. J., Roberts, M. S., Choudhary, J., Barry, S. T., Mazaki, Y., Sabe, H., Morley, S. J., Critchley, D. R., Norman, J. C. (2002). Paxillin Associates with Poly(A)-binding Protein 1 at the Dense Endoplasmic Reticulum and the Leading Edge of Migrating Cells. J. Biol. Chem. 277: 6428-6437 [Abstract] [Full Text]
Hagel, M., George, E. L., Kim, A., Tamimi, R., Opitz, S. L., Turner, C. E., Imamoto, A., Thomas, S. M. (2002). The Adaptor Protein Paxillin Is Essential for Normal Development in the Mouse and Is a Critical Transducer of Fibronectin Signaling. Mol. Cell. Biol. 22: 901-915 [Abstract] [Full Text]
Adams, J. C. (2002). Regulation of protrusive and contractile cell-matrix contacts. J. Cell Sci. 115: 257-265 [Abstract] [Full Text]
Huang, C.-F., Chen, C.-C., Tung, L., Buu, L.-M., Lee, F.-J. S. (2002). The yeast ADP-ribosylation factor GAP, Gcs1p, is involved in maintenance of mitochondrial morphology. J. Cell Sci. 115: 275-282 [Abstract] [Full Text]
Manabe, R.-i., Kovalenko, M., Webb, D. J., Horwitz, A. R. (2002). GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration. J. Cell Sci. 115: 1497-1510 [Abstract] [Full Text]
Szafer, E., Rotman, M., Cassel, D. (2001). Regulation of GTP Hydrolysis on ADP-ribosylation Factor-1 at the Golgi Membrane. J. Biol. Chem. 276: 47834-47839 [Abstract] [Full Text]
Jia, Y., Ransom, R. F., Shibanuma, M., Liu, C., Welsh, M. J., Smoyer, W. E. (2001). Identification and Characterization of hic-5/ARA55 as an hsp27 Binding Protein. J. Biol. Chem. 276: 39911-39918 [Abstract] [Full Text]