Budding Yeast Bub2 Is Localized at Spindle Pole Bodies and Activates the Mitotic Checkpoint via a Different Pathway from Mad2

doi:10.1083/jcb.145.5.979

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© The Rockefeller University Press, 0021-9525/1999//979 $5.00
The Journal of Cell Biology, Volume 145, Number 5, , 1999 979-991

Regular Articles

Budding Yeast Bub2 Is Localized at Spindle Pole Bodies and Activates the Mitotic Checkpoint via a Different Pathway from Mad2

Roberta Fraschini, Elisa Formenti, Giovanna Lucchini, and Simonetta Piatti

Dipartimento di Genetica e Biologia dei Microrganismi, 20133 Milano, Italy

The mitotic checkpoint blocks cell cycle progression beforeanaphase in case of mistakes in the alignment of chromosomeson the mitotic spindle. In budding yeast, the Mad1, 2, 3, andBub1, 2, 3 proteins mediate this arrest. Vertebrate homologuesof Mad1, 2, 3, and Bub1, 3 bind to unattached kinetochoresand prevent progression through mitosis by inhibiting Cdc20/APC-mediatedproteolysis of anaphase inhibitors, like Pds1 and B-type cyclins.We investigated the role of Bub2 in budding yeast mitotic checkpoint.The following observations indicate that Bub2 and Mad1, 2 probably activate the checkpoint via different pathways: (a)unlike the other Mad and Bub proteins, Bub2 localizes at thespindle pole body (SPB) throughout the cell cycle; (b) theeffect of concomitant lack of Mad1 or Mad2 and Bub2 is additive,since nocodazole-treated mad1 bub2 and mad2 bub2 double mutantsrereplicate DNA more rapidly and efficiently than either single mutant; (c) cell cycle progression of bub2 cells in the presenceof nocodazole requires the Cdc26 APC subunit, which, conversely,is not required for mad2 cells in the same conditions. Altogether,our data suggest that activation of the mitotic checkpointblocks progression through mitosis by independent and partiallyredundant mechanisms.

Key Words: budding yeast • Bub2 • mitotic checkpoint • anaphase • anaphase-promoting complex

Abbreviations used in this paper: APC, anaphase-promoting complex; BUB, budding uninhibited by benzimidazole; cyc, cycling; exp, exponential phase; HU, hydroxyurea; MAD, mitotic arrest-deficient; SPB, spindle pole body.

The first two authors contributed equally to this work.

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