Chs7p, a New Protein Involved in the Control of Protein Export from the Endoplasmic Reticulum that Is Specifically Engaged in the Regulation of Chitin Synthesis in Saccharomyces cerevisiae

doi:10.1083/jcb.145.6.1153

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© The Rockefeller University Press, 0021-9525/1999//1153 $5.00
The Journal of Cell Biology, Volume 145, Number 6, , 1999 1153-1163

Article

Chs7p, a New Protein Involved in the Control of Protein Export from the Endoplasmic Reticulum that Is Specifically Engaged in the Regulation of Chitin Synthesis in Saccharomyces cerevisiae

Jose A. Trilla, Angel Durán, and Cesar Roncero

Instituto de Microbiología Bioquímica, Consejo Superior de Investigaciones Científicas/Universidad de Salamanca and Departamento de Microbiología y Genética, Universidad de Salamanca 37007 Salamanca, Spain

The Saccharomyces cerevisiae CHS7 gene encodes an integralmembrane protein located in the ER which is directly involvedin chitin synthesis through the regulation of chitin synthaseIII (CSIII) activity. In the absence of CHS7 product, Chs3p,but not other secreted proteins, is retained in the ER, leadingto a severe defect in CSIII activity and consequently, to areduced rate of chitin synthesis. In addition, chs7 null mutantsshow the yeast phenotypes associated with a lack of chitin:reduced mating efficiency and lack of the chitosan ascosporelayer, clear indications of Chs7p function throughout the S.cerevisiae biological cycle.

CHS3 overexpression does not lead to increased levels of CSIIIbecause the Chs3p excess is retained in the ER. However, jointoverexpression of CHS3 and CHS7 increases the export of Chs3pfrom the ER and this is accompanied by a concomitant increasein CSIII activity, indicating that the amount of Chs7p is alimiting factor for CSIII activity. Accordingly, CHS7 transcription is increased when elevated amounts of chitin synthesis aredetected.

These results show that Chs7p forms part of a new mechanismspecifically involved in Chs3p export from the ER and consequently,in the regulation of CSIII activity.

Key Words: chitin synthesis • chitin synthase III • endoplasmic reticulum export • morphogenesis • cell wall

Abbreviations used in this paper: CS, chitin synthase; cwr, Calcofluor white resistant; GFP, green fluorescent protein; HA, hemagglutinin epitope; ORF, open reading frame; v-ATPase, vacuolar ATPase.

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