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J. Cell Biol.,
Volume 145, Number 7, June 28, 1999 1369-1380
* Service de Biochimie et de Génétique Moléculaire, Commissariat á L'Energie Atomique (CEA)/Saclay, F-91191 Gif-sur-Yvette
Cedex, France; and A collection of yeast strains surviving with
mutant 5S RNA has been constructed. The mutant
strains presented alterations of the nucleolar structure,
with less granular component, and a delocalization of
the 25S rRNA throughout the nucleoplasm. The 5S
RNA mutations affected helix I and resulted in decreased amounts of stable 5S RNA and of the ribosomal 60S subunits. The shortage of 60S subunits was due
to a specific defect in the processing of the 27SB precursor RNA that gives rise to the mature 25S and 5.8S
rRNA. The processing rate of the 27SB pre-rRNA was
specifically delayed, whereas the 27SA and 20S pre-rRNA were processed at a normal rate. The defect was
partially corrected by increasing the amount of mutant
5S RNA. We propose that the 5S RNA is recruited by
the pre-60S particle and that its recruitment is necessary for the efficient processing of the 27SB RNA precursor. Such a mechanism could ensure that all newly
formed mature 60S subunits contain stoichiometric
amounts of the three rRNA components.
Laboratoire de Biologie Moléculaire des Eucaryotes du Centre National de la Recherche Scientifique,
F-31062 Toulouse Cedex, France
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