Sli15 Associates with the Ipl1 Protein Kinase to Promote Proper Chromosome Segregation in Saccharomyces cerevisiae

doi:10.1083/jcb.145.7.1381

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© The Rockefeller University Press, 0021-9525/1999//1381 $5.00
The Journal of Cell Biology, Volume 145, Number 7, , 1999 1381-1394

Regular Article

Sli15 Associates with the Ipl1 Protein Kinase to Promote Proper Chromosome Segregation in Saccharomyces cerevisiae

Jae-hyun Kim, Jung-seog Kang, and Clarence S.M. Chan

Department of Microbiology and Institute for Cellular and Molecular Biology, The University of Texas, Austin, Texas 78712

The conserved Ipl1 protein kinase is essential for proper chromosomesegregation and thus cell viability in the budding yeast Saccharomycescerevisiae. Its human homologue has been implicated in thetumorigenesis of diverse forms of cancer. We show here thatsister chromatids that have separated from each other are notproperly segregated to opposite poles of ipl1-2 cells. Failuresin chromosome segregation are often associated with abnormaldistribution of the spindle pole–associated Nuf2-GFPprotein, thus suggesting a link between potential spindle poledefects and chromosome missegregation in ipl1 mutant cells.A small fraction of ipl1-2 cells also appears to be defectivein nuclear migration or bipolar spindle formation. Ipl1 associates,probably directly, with the novel and essential Sli15 proteinin vivo, and both proteins are localized to the mitotic spindle.Conditional sli15 mutant cells have cytological phenotypesvery similar to those of ipl1 cells, and the ipl1-2 mutationexhibits synthetic lethal genetic interaction with sli15 mutations.sli15 mutant phenotype, like ipl1 mutant phenotype, is partiallysuppressed by perturbations that reduce protein phosphatase1 function. These genetic and biochemical studies indicate thatSli15 associates with Ipl1 to promote its function in chromosomesegregation.

Key Words: chromosome segregation • Ipl1 • Sli15 • protein kinase • Nuf2

Abbreviations used in this paper: GFP, green fluorescent protein; PP1, protein phosphatase 1; TetR-GFP, Tet repressor-green fluorescent protein; Ts^–, temperature-sensitive.

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