Molecular Characterization of Caveolin Association with the Golgi Complex: Identification of a Cis-Golgi Targeting Domain in the Caveolin Molecule

doi:10.1083/jcb.145.7.1443

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J. Cell Biol., Volume 145, Number 7, June 28, 1999 1443-1459

Molecular Characterization of Caveolin Association with the Golgi Complex: Identification of a Cis-Golgi Targeting Domain in the Caveolin Molecule

Robert Luetterforst,^* Espen Stang,^* Natasha Zorzi,^* Amanda Carozzi,^* Michael Way, and Robert G. Parton^*

^* Centre for Microscopy and Microanalysis, Department of Physiology and Pharmacology, and Centre for Molecular and Cellular Biology, University of Queensland, Brisbane, Queensland 4072, Australia; and European Molecular Biology Laboratory, 69012 Heidelberg, Germany

Caveolins are integral membrane proteins which are a major component of caveolae. In addition, caveolins have been proposedto cycle between intracellular compartments and the cell surfacebut the exact trafficking route and targeting information in thecaveolin molecule have not been defined. We show that antibodiesagainst the caveolin scaffolding domain or against the COOH terminusof caveolin-1 show a striking specificity for the Golgi pool ofcaveolin and do not recognize surface caveolin by immunofluorescence.To analyze the Golgi targeting of caveolin in more detail, caveolinmutants were expressed in fibroblasts. Specific mutants lackingthe NH₂ terminus were targeted to the cis Golgi but were not detectablein surface caveolae. Moreover, a 32-amino acid segment of theputative COOH-terminal cytoplasmic domain of caveolin-3 was targetedspecifically and exclusively to the Golgi complex and could targeta soluble heterologous protein, green fluorescent protein, tothis compartment. Palmitoylation-deficient COOH-terminal mutantsshowed negligible association with the Golgi complex. This studydefines unique Golgi targeting information in the caveolin moleculeand identifies the cis Golgi complex as an intermediate compartmenton the caveolin cyclingpathway.

Key words: caveolae; caveolin; Golgi complex; targeting; palmitoylation

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