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© The Rockefeller University Press, 0021-9525/1999//1443 $5.00
The Journal of Cell Biology, Volume 145, Number 7, , 1999 1443-1459

Molecular Characterization of Caveolin Association with the Golgi Complex: Identification of a Cis-Golgi Targeting Domain in the Caveolin Molecule

Robert Luetterforst^*, Espen Stang^*, Natasha Zorzi^*, Amanda Carozzi^*, Michael Way, and Robert G. Parton^*

^* Centre for Microscopy and Microanalysis, Department of Physiology and Pharmacology, and Centre for Molecular and Cellular Biology, University of Queensland, Brisbane, Queensland 4072, Australia; and European Molecular Biology Laboratory, 69012 Heidelberg, Germany

Caveolins are integral membrane proteins which are a major component of caveolae. In addition, caveolins have been proposed to cycle between intracellular compartments and the cell surface but the exact trafficking route and targeting information in the caveolin molecule have not been defined. We show that antibodies against the caveolin scaffolding domain or against the COOH terminus of caveolin-1 show a striking specificity for the Golgi pool of caveolin and do not recognize surface caveolin by immunofluorescence. To analyze the Golgi targeting of caveolin in more detail, caveolin mutants were expressed in fibroblasts. Specific mutants lacking the NH₂ terminus were targeted to the cis Golgi but were not detectable in surface caveolae. Moreover, a 32–amino acid segment of the putative COOH-terminal cytoplasmic domain of caveolin-3 was targeted specifically and exclusively to the Golgi complex and could target a soluble heterologous protein, green fluorescent protein, to this compartment. Palmitoylation-deficient COOH-terminal mutants showed negligible association with the Golgi complex. This study defines unique Golgi targeting information in the caveolin molecule and identifies the cis Golgi complex as an intermediate compartment on the caveolin cycling pathway.

Key Words: caveolae • caveolin • Golgi complex • targeting • palmitoylation

Abbreviations used in this paper: cav-1, -2, -3, caveolin-1, -2, -3; GFP, green fluorescent protein; SFV, Semliki Forest Virus; WT, wild-type.

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