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© The Rockefeller University Press, 0021-9525/1999//165 $5.00
The Journal of Cell Biology, Volume 146, Number 1, , 1999 165-180

Original Article

Drosophila roadblock and Chlamydomonas Lc7

: A Conserved Family of Dynein-Associated Proteins Involved in Axonal Transport, Flagellar Motility, and Mitosis



Aaron B. Bowmana, Ramila S. Patel-Kingc, Sharon E. Benashskic, J. Michael McCafferyb, Lawrence S.B. Goldsteina, and Stephen M. Kingc

a Howard Hughes Medical Institute, Division of Cellular and Molecular Medicine, Department of Pharmacology, University of California San Diego, La Jolla, California 92093-0683
b Integrated Imaging Center, Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218
c Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032-3305
Howard Hughes Medical Institute, Division of Cellular and Molecular Medicine, Department of Pharmacology, 334 m/c 0683, University of California San Diego, La Jolla, CA 92093-0683.(619) 534-9701(619) 534-9702

lgoldstein{at}ucsd.edu

Eukaryotic organisms utilize microtubule-dependent motors of the kinesin and dynein superfamilies to generate intracellular movement. To identify new genes involved in the regulation of axonal transport in Drosophila melanogaster, we undertook a screen based upon the sluggish larval phenotype of known motor mutants. One of the mutants identified in this screen, roadblock (robl), exhibits diverse defects in intracellular transport including axonal transport and mitosis. These defects include intra-axonal accumulations of cargoes, severe axonal degeneration, and aberrant chromosome segregation. The gene identified by robl encodes a 97–amino acid polypeptide that is 57% identical (70% similar) to the 105–amino acid Chlamydomonas outer arm dynein–associated protein LC7, also reported here. Both robl and LC7 have homology to several other genes from fruit fly, nematode, and mammals, but not Saccharomyces cerevisiae. Furthermore, we demonstrate that members of this family of proteins are associated with both flagellar outer arm dynein and Drosophila and rat brain cytoplasmic dynein. We propose that roadblock/LC7 family members may modulate specific dynein functions.

Key Words: axonal transport • mitosis • dynein • ATPase • nerve degeneration • flagella

© 1999 The Rockefeller University Press

1.used in this paper: BDGP, Berkeley Drosophila Genome Project; bxd, late RNA encoded bithoraxoid protein; ChAT, choline acetyltransferase; DLC, dynein light chain; EMS, ethyl methanesulfonate; EST, expressed sequence tag; IC74, 74-kD cytoplasmic dynein intermediate chain; khc, kinesin heavy chain; NCBI, National Center for Biotechnology Information; robl, roadblock; SYT, synaptotagmin; VG, ventral ganglion

Reprint requests may be sent to either L.S.B. Goldstein or S.M. King.


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