Shc and Fak Differentially Regulate Cell Motility and Directionality Modulated by Pten

doi:10.1083/jcb.146.2.389

This Article

	Full Text
	Full Text (PDF, 392K)
	PPT slides of all figures
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Gu, J.
	Articles by Yamada, K. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Gu, J.
	Articles by Yamada, K. M.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/1999//389 $5.00
The Journal of Cell Biology, Volume 146, Number 2, , 1999 389-404

Original Article

Shc and Fak Differentially Regulate Cell Motility and Directionality Modulated by Pten

Jianguo Gu^a, Masahito Tamura^a, Roumen Pankov^a, Erik H.J. Danen^a, Takahisa Takino^a, Kazue Matsumoto^a, and Kenneth M. Yamada^a

^a Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892-4370
Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Building 30, Room 421, 30 Convent Drive MSC 4370, Bethesda, MD 20892-4370.(301) 402-0897(301) 496-9124

ky4w{at}nih.gov

Cell migration is modulated by regulatory molecules such asgrowth factors, oncogenes, and the tumor suppressor PTEN. Wepreviously described inhibition of cell migration by PTEN andrestoration of motility by focal adhesion kinase (FAK) and p130Crk-associated substrate (p130^Cas). We now report a novel pathwayregulating random cell motility involving Shc and mitogen-activatedprotein (MAP) kinase, which is downmodulated by PTEN and additiveto a FAK pathway regulating directional migration. Overexpressionof Shc or constitutively activated MEK1 in PTEN- reconstitutedU87-MG cells stimulated integrin- mediated MAP kinase activationand cell migration. Conversely, overexpression of dominant negativeShc inhibited cell migration; Akt appeared uninvolved. PTENdirectly dephosphorylated Shc. The migration induced by FAKor p130^Cas was directionally persistent and involved extensiveorganization of actin microfilaments and focal adhesions. Incontrast, Shc or MEK1 induced a random type of motility associatedwith less actin cytoskeletal and focal adhesion organization.These results identify two distinct, additive pathways regulatingcell migration that are downregulated by tumor suppressor PTEN:one involves Shc, a MAP kinase pathway, and random migration,whereas the other involves FAK, p130^Cas, more extensive actincytoskeletal organization, focal contacts, and directionallypersistent cell motility. Integration of these pathways providesan intracellular mechanism for regulating the speed and thedirectionality of cell migration.

Key Words: Shc • focal adhesion kinase • integrin • cell migration • PTEN

1.used in this paper: Csk, COOH-terminal Src kinase; ERK, extracellularsignal-related kinase; FAK, focal adhesion kinase; FRNK, dominantnegative FAK truncation; GFP, green fluorescent protein; HA,hemagglutinin; MAP, mitogen-activated protein; MEK, MAP or ERKkinase; p130Cas, p130 Crk-associated substrate; PIP3, phosphatidylinositol3,4,5-trisphosphate

The present address of J. Gu is Division of Protein Chemistry,Institute for Protein Research, Osaka University, 3-2 Yamadaoka,Suita, Osaka 565, Japan. The present address of M. Tamura isSecond Department of Internal Medicine, University of Occupationaland Environmental Health, 1-1 Iseigaoka, Yahata-nishi, Kitakyushu807-8555, Japan. The present address of E.H.J. Danen is Divisionof Cell Biology, Netherlands Cancer Institute, Plesmanlaan 121,1066 CX Amsterdam, The Netherlands.

CiteULike

Complore

Connotea

Del.icio.us Add to Digg

Digg

Facebook

Technorati

Twitter What's this?

This article has been cited by other articles:

Miyamoto, T., Tamura, M., Kabashima, N., Serino, R., Shibata, T., Furuno, Y., Miyazaki, M., Baba, R., Sato, N., Doi, Y., Okazaki, M., Otsuji, Y. (2009). An integrin-activating peptide, PHSRN, ameliorates inhibitory effects of conventional peritoneal dialysis fluids on peritoneal wound healing. Nephrol Dial Transplant 0: gfp601v1-gfp601 [Abstract] [Full Text]
Hill, J. W., Xu, Y., Preitner, F., Fukuda, M., Cho, Y.-R., Luo, J., Balthasar, N., Coppari, R., Cantley, L. C., Kahn, B. B., Zhao, J. J., Elmquist, J. K. (2009). Phosphatidyl Inositol 3-Kinase Signaling in Hypothalamic Proopiomelanocortin Neurons Contributes to the Regulation of Glucose Homeostasis. Endocrinology 150: 4874-4882 [Abstract] [Full Text]
Pramanik, Md. K., Iijima, M., Iwadate, Y., Yumura, S. (2009). PTEN is a mechanosensing signal transducer for myosin II localization in Dictyostelium cells. GENES CELLS 14: 821-834 [Abstract] [Full Text]
Tomar, A., Lim, S.-T., Lim, Y., Schlaepfer, D. D. (2009). A FAK-p120RasGAP-p190RhoGAP complex regulates polarity in migrating cells. J. Cell Sci. 122: 1852-1862 [Abstract] [Full Text]
Ma, F., Zhang, D., Yang, H., Sun, H., Wu, W., Gan, Y., Balducci, J., Wei, Y.-q., Zhao, X., Huang, Y. (2009). Endothelial cell-specific molecule 2 (ECSM2) modulates actin remodeling and epidermal growth factor receptor signaling.. GENES CELLS 14: 281-293 [Abstract] [Full Text]
Li, Y., Guessous, F., DiPierro, C., Zhang, Y., Mudrick, T., Fuller, L., Johnson, E., Marcinkiewicz, L., Engelhardt, M., Kefas, B., Schiff, D., Kim, J., Abounader, R. (2009). Interactions between PTEN and the c-Met pathway in glioblastoma and implications for therapy. Molecular Cancer Therapeutics 8: 376-385 [Abstract] [Full Text]
Xi, G., Shen, X., Clemmons, D. R. (2008). p66shc Negatively Regulates Insulin-Like Growth Factor I Signal Transduction via Inhibition of p52shc Binding to Src Homology 2 Domain-Containing Protein Tyrosine Phosphatase Substrate-1 Leading to Impaired Growth Factor Receptor-Bound Protein-2 Membrane Recruitment. Mol. Endocrinol. 22: 2162-2175 [Abstract] [Full Text]
Inoue-Narita, T., Hamada, K., Sasaki, T., Hatakeyama, S., Fujita, S., Kawahara, K., Sasaki, M., Kishimoto, H., Eguchi, S., Kojima, I., Beermann, F., Kimura, T., Osawa, M., Itami, S., Mak, T. W., Nakano, T., Manabe, M., Suzuki, A. (2008). Pten Deficiency in Melanocytes Results in Resistance to Hair Graying and Susceptibility to Carcinogen-Induced Melanomagenesis. Cancer Res. 68: 5760-5768 [Abstract] [Full Text]
Oudit, G. Y., Kassiri, Z., Zhou, J., Liu, Q. C., Liu, P. P., Backx, P. H., Dawood, F., Crackower, M. A., Scholey, J. W., Penninger, J. M. (2008). Loss of PTEN attenuates the development of pathological hypertrophy and heart failure in response to biomechanical stress. Cardiovasc Res 78: 505-514 [Abstract] [Full Text]
Sprague, C. R., Fraley, T. S., Jang, H. S., Lal, S., Greenwood, J. A. (2008). Phosphoinositide Binding to the Substrate Regulates Susceptibility to Proteolysis by Calpain. J. Biol. Chem. 283: 9217-9223 [Abstract] [Full Text]
Rottmann, S., Speckgens, S., Luscher-Firzlaff, J., Luscher, B. (2008). Inhibition of apoptosis by MAD1 is mediated by repression of the PTEN tumor suppressor gene. FASEB J. 22: 1124-1134 [Abstract] [Full Text]
Dey, N., Crosswell, H. E., De, P., Parsons, R., Peng, Q., Su, J. D., Durden, D. L. (2008). The Protein Phosphatase Activity of PTEN Regulates Src Family Kinases and Controls Glioma Migration. Cancer Res. 68: 1862-1871 [Abstract] [Full Text]
Hayashida, T., Wu, M.-H., Pierce, A., Poncelet, A.-C., Varga, J., Schnaper, H. W. (2007). MAP-kinase activity necessary for TGFbeta1-stimulated mesangial cell type I collagen expression requires adhesion-dependent phosphorylation of FAK tyrosine 397. J. Cell Sci. 120: 4230-4240 [Abstract] [Full Text]
Boyle, E. C., Brown, N. F., Brumell, J. H., Finlay, B. B. (2007). Src homology domain 2 adaptors affect adherence of Salmonella enterica serovar Typhimurium to non-phagocytic cells. Microbiology 153: 3517-3526 [Abstract] [Full Text]
Maa, M.-C., Lee, J.-C., Chen, Y.-J., Chen, Y.-J., Lee, Y.-C., Wang, S.-T., Huang, C.-C., Chow, N.-H., Leu, T.-H. (2007). EPS8 Facilitates Cellular Growth and Motility of Colon Cancer Cells by Increasing the Expression and Activity of Focal Adhesion Kinase. J. Biol. Chem. 282: 19399-19409 [Abstract] [Full Text]
Blanco-Aparicio, C., Renner, O., Leal, J. F.M., Carnero, A. (2007). PTEN, more than the AKT pathway. Carcinogenesis 28: 1379-1386 [Abstract] [Full Text]
Wu, Z, McRoberts, K., Theodorescu, D (2007). The role of PTEN in prostate cancer cell tropism to the bone micro-environment. Carcinogenesis 28: 1393-1400 [Abstract] [Full Text]
Katiyar, S., Jiao, X., Wagner, E., Lisanti, M. P., Pestell, R. G. (2007). Somatic Excision Demonstrates that c-Jun Induces Cellular Migration and Invasion through Induction of Stem Cell Factor. Mol. Cell. Biol. 27: 1356-1369 [Abstract] [Full Text]
Tsuruta, H., Kishimoto, H., Sasaki, T., Horie, Y., Natsui, M., Shibata, Y., Hamada, K., Yajima, N., Kawahara, K., Sasaki, M., Tsuchiya, N., Enomoto, K., Mak, T. W., Nakano, T., Habuchi, T., Suzuki, A. (2006). Hyperplasia and Carcinomas in Pten-Deficient Mice and Reduced PTEN Protein in Human Bladder Cancer Patients.. Cancer Res. 66: 8389-8396 [Abstract] [Full Text]
Packer, L., Pavey, S., Parker, A., Stark, M., Johansson, P., Clarke, B., Pollock, P., Ringner, M., Hayward, N. (2006). Osteopontin is a downstream effector of the PI3-kinase pathway in melanomas that is inversely correlated with functional PTEN. Carcinogenesis 27: 1778-1786 [Abstract] [Full Text]
Desban, N., Lissitzky, J.-C., Rousselle, P., Duband, J.-L. (2006). {alpha}1{beta}1-integrin engagement to distinct laminin-1 domains orchestrates spreading, migration and survival of neural crest cells through independent signaling pathways. J. Cell Sci. 119: 3206-3218 [Abstract] [Full Text]
Moissoglu, K., Slepchenko, B. M., Meller, N., Horwitz, A. F., Schwartz, M. A. (2006). In Vivo Dynamics of Rac-Membrane Interactions. Mol. Biol. Cell 17: 2770-2779 [Abstract] [Full Text]
Park, C. C., Zhang, H., Pallavicini, M., Gray, J. W., Baehner, F., Park, C. J., Bissell, M. J. (2006). {beta}1 Integrin Inhibitory Antibody Induces Apoptosis of Breast Cancer Cells, Inhibits Growth, and Distinguishes Malignant from Normal Phenotype in Three Dimensional Cultures and In vivo. Cancer Res. 66: 1526-1535 [Abstract] [Full Text]
Mitola, S., Brenchio, B., Piccinini, M., Tertoolen, L., Zammataro, L., Breier, G., Rinaudo, M. T., den Hertog, J., Arese, M., Bussolino, F. (2006). Type I Collagen Limits VEGFR-2 Signaling by a SHP2 Protein-Tyrosine Phosphatase-Dependent Mechanism 1. Circ. Res. 98: 45-54 [Abstract] [Full Text]
White, E. S., Atrasz, R. G., Hu, B., Phan, S. H., Stambolic, V., Mak, T. W., Hogaboam, C. M., Flaherty, K. R., Martinez, F. J., Kontos, C. D., Toews, G. B. (2006). Negative Regulation of Myofibroblast Differentiation by PTEN (Phosphatase and Tensin Homolog Deleted on Chromosome 10). Am. J. Respir. Crit. Care Med. 173: 112-121 [Abstract] [Full Text]
Vogelmann, R., Nguyen-tat, M.-D., Giehl, K., Adler, G., Wedlich, D., Menke, A. (2005). TGF{beta}-induced downregulation of E-cadherin-based cell-cell adhesion depends on PI3-kinase and PTEN. J. Cell Sci. 118: 4901-4912 [Abstract] [Full Text]
Wang, D., You, M. (2005). Five Loci, SLT1 to SLT5, Controlling the Susceptibility to Spontaneously Occurring Lung Cancer in Mice. Cancer Res. 65: 8158-8165 [Abstract] [Full Text]
Koblinski, J. E., Kaplan-Singer, B. R., VanOsdol, S. J., Wu, M., Engbring, J. A., Wang, S., Goldsmith, C. M., Piper, J. T., Vostal, J. G., Harms, J. F., Welch, D. R., Kleinman, H. K. (2005). Endogenous Osteonectin/SPARC/BM-40 Expression Inhibits MDA-MB-231 Breast Cancer Cell Metastasis. Cancer Res. 65: 7370-7377 [Abstract] [Full Text]
White, E. S., Atrasz, R. G., Dickie, E. G., Aronoff, D. M., Stambolic, V., Mak, T. W., Moore, B. B., Peters-Golden, M. (2005). Prostaglandin E2 Inhibits Fibroblast Migration by E-Prostanoid 2 Receptor-Mediated Increase in PTEN Activity. Am. J. Respir. Cell Mol. Bio. 32: 135-141 [Abstract] [Full Text]
Khyrul, W. A. K. M., LaLonde, D. P., Brown, M. C., Levinson, H., Turner, C. E. (2004). The Integrin-linked Kinase Regulates Cell Morphology and Motility in a Rho-associated Kinase-dependent Manner. J. Biol. Chem. 279: 54131-54139 [Abstract] [Full Text]
Lacalle, R. A., Gomez-Mouton, C., Barber, D. F., Jimenez-Baranda, S., Mira, E., Martinez-A., C., Carrera, A. C., Manes, S. (2004). PTEN regulates motility but not directionality during leukocyte chemotaxis. J. Cell Sci. 117: 6207-6215 [Abstract] [Full Text]
Natalicchio, A., Laviola, L., De Tullio, C., Renna, L. A., Montrone, C., Perrini, S., Valenti, G., Procino, G., Svelto, M., Giorgino, F. (2004). Role of the p66Shc Isoform in Insulin-like Growth Factor I Receptor Signaling through MEK/Erk and Regulation of Actin Cytoskeleton in Rat Myoblasts. J. Biol. Chem. 279: 43900-43909 [Abstract] [Full Text]
Lin, H.-K., Hu, Y.-C., Lee, D. K., Chang, C. (2004). Regulation of Androgen Receptor Signaling by PTEN (Phosphatase and Tensin Homolog Deleted on Chromosome 10) Tumor Suppressor through Distinct Mechanisms in Prostate Cancer Cells. Mol. Endocrinol. 18: 2409-2423 [Abstract] [Full Text]
Westhoff, M. A., Serrels, B., Fincham, V. J., Frame, M. C., Carragher, N. O. (2004). Src-Mediated Phosphorylation of Focal Adhesion Kinase Couples Actin and Adhesion Dynamics to Survival Signaling. Mol. Cell. Biol. 24: 8113-8133 [Abstract] [Full Text]
Zhang, Z., Izaguirre, G., Lin, S.-Y., Lee, H. Y., Schaefer, E., Haimovich, B. (2004). The Phosphorylation of Vinculin on Tyrosine Residues 100 and 1065, Mediated by Src Kinases, Affects Cell Spreading. Mol. Biol. Cell 15: 4234-4247 [Abstract] [Full Text]
Iijima, M., Huang, Y. E., Luo, H. R., Vazquez, F., Devreotes, P. N. (2004). Novel Mechanism of PTEN Regulation by Its Phosphatidylinositol 4,5-Bisphosphate Binding Motif Is Critical for Chemotaxis. J. Biol. Chem. 279: 16606-16613 [Abstract] [Full Text]
Mahimainathan, L., Choudhury, G. G. (2004). Inactivation of Platelet-derived Growth Factor Receptor by the Tumor Suppressor PTEN Provides a Novel Mechanism of Action of the Phosphatase. J. Biol. Chem. 279: 15258-15268 [Abstract] [Full Text]
Audero, E., Cascone, I., Maniero, F., Napione, L., Arese, M., Lanfrancone, L., Bussolino, F. (2004). Adaptor ShcA Protein Binds Tyrosine Kinase Tie2 Receptor and Regulates Migration and Sprouting but Not Survival of Endothelial Cells. J. Biol. Chem. 279: 13224-13233 [Abstract] [Full Text]
Gu, J., Zhao, Y., Isaji, T., Shibukawa, Y., Ihara, H., Takahashi, M., Ikeda, Y., Miyoshi, E., Honke, K., Taniguchi, N. (2004). {beta}1,4-N-Acetylglucosaminyltransferase III down-regulates neurite outgrowth induced by costimulation of epidermal growth factor and integrins through the Ras/ERK signaling pathway in PC12 cells. Glycobiology 14: 177-186 [Abstract] [Full Text]
Strasly, M., Doronzo, G., Capello, P., Valdembri, D., Arese, M., Mitola, S., Moore, P., Alessandri, G., Giovarelli, M., Bussolino, F. (2004). CCL16 activates an angiogenic program in vascular endothelial cells. Blood 103: 40-49 [Abstract] [Full Text]
Moissoglu, K., Gelman, I. H. (2003). v-Src Rescues Actin-based Cytoskeletal Architecture and Cell Motility and Induces Enhanced Anchorage Independence during Oncogenic Transformation of Focal Adhesion Kinase-null Fibroblasts. J. Biol. Chem. 278: 47946-47959 [Abstract] [Full Text]
Zetser, A., Bashenko, Y., Miao, H.-Q., Vlodavsky, I., Ilan, N. (2003). Heparanase Affects Adhesive and Tumorigenic Potential of Human Glioma Cells. Cancer Res. 63: 7733-7741 [Abstract] [Full Text]
Nakayamada, S., Okada, Y., Saito, K., Tamura, M., Tanaka, Y. (2003). {beta}1 Integrin/Focal Adhesion Kinase-mediated Signaling Induces Intercellular Adhesion Molecule 1 and Receptor Activator of Nuclear Factor {kappa}B Ligand on Osteoblasts and Osteoclast Maturation. J. Biol. Chem. 278: 45368-45374 [Abstract] [Full Text]
Davol, P. A., Bagdasaryan, R., Elfenbein, G. J., Maizel, A. L., Frackelton, A. R. Jr. (2003). Shc Proteins Are Strong, Independent Prognostic Markers for Both Node-Negative and Node-Positive Primary Breast Cancer. Cancer Res. 63: 6772-6783 [Abstract] [Full Text]
Goberdhan, D. C.I., Wilson, C. (2003). PTEN: tumour suppressor, multifunctional growth regulator and more. Hum Mol Genet 12: R239-248 [Abstract] [Full Text]
Katz, B.-Z., Romer, L., Miyamoto, S., Volberg, T., Matsumoto, K., Cukierman, E., Geiger, B., Yamada, K. M. (2003). Targeting Membrane-localized Focal Adhesion Kinase to Focal Adhesions: ROLES OF TYROSINE PHOSPHORYLATION AND SRC FAMILY KINASES. J. Biol. Chem. 278: 29115-29120 [Abstract] [Full Text]
Wu, W., Wang, X., Zhang, W., Reed, W., Samet, J. M., Whang, Y. E., Ghio, A. J. (2003). Zinc-induced PTEN Protein Degradation through the Proteasome Pathway in Human Airway Epithelial Cells. J. Biol. Chem. 278: 28258-28263 [Abstract] [Full Text]
Zhang, X. A., He, B., Zhou, B., Liu, L. (2003). Requirement of the p130CAS-Crk Coupling for Metastasis Suppressor KAI1/CD82-mediated Inhibition of Cell Migration. J. Biol. Chem. 278: 27319-27328 [Abstract] [Full Text]
Hunger-Glaser, I., Salazar, E. P., Sinnett-Smith, J., Rozengurt, E. (2003). Bombesin, Lysophosphatidic Acid, and Epidermal Growth Factor Rapidly Stimulate Focal Adhesion Kinase Phosphorylation at Ser-910: REQUIREMENT FOR ERK ACTIVATION. J. Biol. Chem. 278: 22631-22643 [Abstract] [Full Text]
Engelholm, L. H., List, K., Netzel-Arnett, S., Cukierman, E., Mitola, D. J., Aaronson, H., Kjoller, L., Larsen, J. K., Yamada, K. M., Strickland, D. K., Holmbeck, K., Dano, K., Birkedal-Hansen, H., Behrendt, N., Bugge, T. H. (2003). uPARAP/Endo180 is essential for cellular uptake of collagen and promotes fibroblast collagen adhesion. JCB 160: 1009-1015 [Abstract] [Full Text]
Lipinski, C. A., Tran, N. L., Bay, C., Kloss, J., McDonough, W. S., Beaudry, C., Berens, M. E., Loftus, J. C. (2003). Differential Role of Proline-Rich Tyrosine Kinase 2 and Focal Adhesion Kinase in Determining Glioblastoma Migration and Proliferation. Mol Cancer Res 1: 323-332 [Abstract] [Full Text]
Gupta, R., Ting, J. T. L., Sokolov, L. N., Johnson, S. A., Luan, S. (2002). A Tumor Suppressor Homolog, AtPTEN1, Is Essential for Pollen Development in Arabidopsis. Plant Cell 14: 2495-2507 [Abstract] [Full Text]
Abe, J.-i., Berk, B. C. (2002). Hypoxia and HIF-1{alpha} Stability: Another Stress-Sensing Mechanism for Shc. Circ. Res. 91: 4-6 [Full Text]
Giannone, G., Ronde, P., Gaire, M., Haiech, J., Takeda, K. (2002). Calcium Oscillations Trigger Focal Adhesion Disassembly in Human U87 Astrocytoma Cells. J. Biol. Chem. 277: 26364-26371 [Abstract] [Full Text]
Jung, F., Haendeler, J., Hoffmann, J., Reissner, A., Dernbach, E., Zeiher, A. M., Dimmeler, S. (2002). Hypoxic Induction of the Hypoxia-Inducible Factor Is Mediated via the Adaptor Protein Shc in Endothelial Cells. Circ. Res. 91: 38-45 [Abstract] [Full Text]
Fox, J. A., Ung, K., Tanlimco, S. G., Jirik, F. R. (2002). Disruption of a Single Pten Allele Augments the Chemotactic Response of B Lymphocytes to Stromal Cell-Derived Factor-1. J. Immunol. 169: 49-54 [Abstract] [Full Text]
Knobbe, C. B., Merlo, A., Reifenberger, G. (2002). Pten signaling in gliomas. Neuro Oncology 4: 196-211 [Abstract]
Sun, S., Steinberg, B. M. (2002). PTEN is a negative regulator of STAT3 activation in human papillomavirus-infected cells. J. Gen. Virol. 83: 1651-1658 [Abstract] [Full Text]
Davies, M. A., Kim, S. J., Parikh, N. U., Dong, Z., Bucana, C. D., Gallick, G. E. (2002). Adenoviral-mediated Expression of MMAC/PTEN Inhibits Proliferation and Metastasis of Human Prostate Cancer Cells. Clin. Cancer Res. 8: 1904-1914 [Abstract] [Full Text]
Gu, J., Fujibayashi, A., Yamada, K. M., Sekiguchi, K. (2002). Laminin-10/11 and Fibronectin Differentially Prevent Apoptosis Induced by Serum Removal via Phosphatidylinositol 3-Kinase/Akt- and MEK1/ERK-dependent Pathways. J. Biol. Chem. 277: 19922-19928 [Abstract] [Full Text]
Li, S., Butler, P., Wang, Y., Hu, Y., Han, D. C., Usami, S., Guan, J.-L., Chien, S. (2002). The role of the dynamics of focal adhesion kinase in the mechanotaxis of endothelial cells. Proc. Natl. Acad. Sci. USA 99: 3546-3551 [Abstract] [Full Text]
Stocker, H., Andjelkovic, M., Oldham, S., Laffargue, M., Wymann, M. P., Hemmings, B. A., Hafen, E. (2002). Living with Lethal PIP3 Levels: Viability of Flies Lacking PTEN Restored by a PH Domain Mutation in Akt/PKB. Science 295: 2088-2091 [Abstract] [Full Text]
Prasad, N., Topping, R. S., Decker, S. J. (2002). Src family tyrosine kinases regulate adhesion-dependent tyrosine phosphorylation of 5'-inositol phosphatase SHIP2 during cell attachment and spreading on collagen I. J. Cell Sci. 115: 3807-3815 [Abstract] [Full Text]
Song, R. X.-D., McPherson, R. A., Adam, L., Bao, Y., Shupnik, M., Kumar, R., Santen, R. J. (2002). Linkage of Rapid Estrogen Action to MAPK Activation by ER{alpha}-Shc Association and Shc Pathway Activation. Mol. Endocrinol. 16: 116-127 [Abstract] [Full Text]
Urbich, C., Dernbach, E., Reissner, A., Vasa, M., Zeiher, A. M., Dimmeler, S. (2002). Shear Stress-Induced Endothelial Cell Migration Involves Integrin Signaling Via the Fibronectin Receptor Subunits {alpha}5 and {beta}1. Arterioscler. Thromb. Vasc. Bio. 22: 69-75 [Abstract] [Full Text]
Mariotti, A., Kedeshian, P. A., Dans, M., Curatola, A. M., Gagnoux-Palacios, L., Giancotti, F. G. (2001). EGF-R signaling through Fyn kinase disrupts the function of integrin {alpha}6{beta}4 at hemidesmosomes: role in epithelial cell migration and carcinoma invasion. JCB 155: 447-458 [Abstract] [Full Text]
Hauck, C. R., Sieg, D. J., Hsia, D. A., Loftus, J. C., Gaarde, W. A., Monia, B. P., Schlaepfer, D. D. (2001). Inhibition of Focal Adhesion Kinase Expression or Activity Disrupts Epidermal Growth Factor-stimulated Signaling Promoting the Migration of Invasive Human Carcinoma Cells. Cancer Res. 61: 7079-7090 [Abstract] [Full Text]
Wang, H.-B., Dembo, M., Hanks, S. K., Wang, Y.-l. (2001). Focal adhesion kinase is involved in mechanosensing during fibroblast migration. Proc. Natl. Acad. Sci. USA 98: 11295-11300 [Abstract] [Full Text]
Zhu, X., Kwon, C.-H., Schlosshauer, P. W., Ellenson, L. H., Baker, S. J. (2001). PTEN Induces G1 Cell Cycle Arrest and Decreases Cyclin D3 Levels in Endometrial Carcinoma Cells. Cancer Res. 61: 4569-4575 [Abstract] [Full Text]
Kurose, K., Zhou, X.-P., Araki, T., Cannistra, S. A., Maher, E. R., Eng, C. (2001). Frequent Loss of PTEN Expression Is Linked to Elevated Phosphorylated Akt Levels, but Not Associated with p27 and Cyclin D1 Expression, in Primary Epithelial Ovarian Carcinomas. Am. J. Pathol. 158: 2097-2106 [Abstract] [Full Text]
Pixley, F. J., Lee, P. S. W., Condeelis, J. S., Stanley, E. R. (2001). Protein Tyrosine Phosphatase {phi} Regulates Paxillin Tyrosine Phosphorylation and Mediates Colony-Stimulating Factor 1-Induced Morphological Changes in Macrophages. Mol. Cell. Biol. 21: 1795-1809 [Abstract] [Full Text]
Yasuda, M., Tanaka, Y., Tamura, M., Fujii, K., Sugaya, M., So, T., Takenoyama, M., Yasumoto, K. (2001). Stimulation of {beta}1 Integrin Down-Regulates ICAM-1 Expression and ICAM-1-dependent Adhesion of Lung Cancer Cells through Focal Adhesion Kinase. Cancer Res. 61: 2022-2030 [Abstract] [Full Text]
Klingbeil, C. K., Hauck, C. R., Hsia, D. A., Jones, K.C., Reider, S. R., Schlaepfer, D. D. (2001). Targeting Pyk2 to {beta}1-Integrin-Containing Focal Contacts Rescues Fibronectin-Stimulated Signaling and Haptotactic Motility Defects of Focal Adhesion Kinase-Null Cells. JCB 152: 97-110 [Abstract] [Full Text]
Yamada, K. M., Araki, M. (2001). Tumor suppressor PTEN: modulator of cell signaling, growth, migration and apoptosis. J. Cell Sci. 114: 2375-2382 [Abstract] [Full Text]
Alahari, S. K., Lee, J. W., Juliano, R. L. (2000). Nischarin, a Novel Protein That Interacts with the Integrin {alpha}5 Subunit and Inhibits Cell Migration. JCB 151: 1141-1154 [Abstract] [Full Text]
Zhou, X.-P., Gimm, O., Hampel, H., Niemann, T., Walker, M. J., Eng, C. (2000). Epigenetic PTEN Silencing in Malignant Melanomas without PTEN Mutation. Am. J. Pathol. 157: 1123-1128 [Abstract] [Full Text]
Yano, H., Uchida, H., Iwasaki, T., Mukai, M., Akedo, H., Nakamura, K., Hashimoto, S., Sabe, H. (2000). Paxillin alpha and Crk-associated substrate exert opposing effects on cell migration and contact inhibition of growth through tyrosine phosphorylation. Proc. Natl. Acad. Sci. USA 97: 9076-9081 [Abstract] [Full Text]
Holway, A. H., Rieger-Christ, K. M., Miner, W. R., Cain, J. W., Dugan, J. M., Pezza, J. A., Silverman, M. L., Shapter, A., McLellan, R., Summerhayes, I. C. (2000). Somatic Mutation of PTEN in Vulvar Cancer. Clin. Cancer Res. 6: 3228-3235 [Abstract] [Full Text]
Boney, C. M., Gruppuso, P. A., Faris, R. A., Frackelton, A. R. Jr. (2000). The Critical Role of Shc in Insulin-Like Growth Factor-I-Mediated Mitogenesis and Differentiation in 3T3-L1 Preadipocytes. Mol. Endocrinol. 14: 805-813 [Abstract] [Full Text]
R.-BORLADO, L., REDONDO, C., ALVAREZ, B., JIMENEZ, C., CRIADO, L. M., FLORES, J., MARCOS, M. A. R., MARTINEZ-A, C., BALOMENOS, D., CARRERA, A. C. (2000). Increased phosphoinositide 3-kinase activity induces a lymphoproliferative disorder and contributes to tumor generation in vivo. FASEB J. 14: 895-903 [Abstract] [Full Text]
Goberdhan, D. C.I., Paricio, N., Goodman, E. C., Mlodzik, M., Wilson, C. (1999). Drosophila tumor suppressor PTEN controls cell size and number by antagonizing the Chico/PI3-kinase signaling pathway. Genes Dev. 13: 3244-3258 [Abstract] [Full Text]
Tamura, M., Gu, J., Tran, H., Yamada, K. M. (1999). PTEN Gene and Integrin Signaling in Cancer. JNCI J Natl Cancer Inst 91: 1820-1828 [Abstract] [Full Text]
Giancotti, F. G., Ruoslahti, E. (1999). Integrin Signaling. Science 285: 1028-1033 [Abstract] [Full Text]
Huang, H, Potter, C., Tao, W, Li, D., Brogiolo, W, Hafen, E, Sun, H, Xu, T (1999). PTEN affects cell size, cell proliferation and apoptosis during Drosophila eye development. Development 126: 5365-5372 [Abstract]
Barberis, L., Wary, K. K., Fiucci, G., Liu, F., Hirsch, E., Brancaccio, M., Altruda, F., Tarone, G., Giancotti, F. G. (2000). Distinct Roles of the Adaptor Protein Shc and Focal Adhesion Kinase in Integrin Signaling to ERK. J. Biol. Chem. 275: 36532-36540 [Abstract] [Full Text]
Zheng, D.-Q., Woodard, A. S., Tallini, G., Languino, L. R. (2000). Substrate Specificity of alpha vbeta 3 Integrin-mediated Cell Migration and Phosphatidylinositol 3-Kinase/AKT Pathway Activation. J. Biol. Chem. 275: 24565-24574 [Abstract] [Full Text]
Cowan, K. J., Law, D. A., Phillips, D. R. (2000). Identification of Shc as the Primary Protein Binding to the Tyrosine-phosphorylated beta 3 Subunit of alpha IIbbeta 3 during Outside-in Integrin Platelet Signaling. J. Biol. Chem. 275: 36423-36429 [Abstract] [Full Text]
Hermanns, H. M., Radtke, S., Schaper, F., Heinrich, P. C., Behrmann, I. (2000). Non-redundant Signal Transduction of Interleukin-6-type Cytokines. THE ADAPTER PROTEIN Shc IS SPECIFICALLY RECRUITED TO THE ONCOSTATIN M RECEPTOR. J. Biol. Chem. 275: 40742-40748 [Abstract] [Full Text]
Hauck, C. R., Hsia, D. A., Schlaepfer, D. D. (2000). Focal Adhesion Kinase Facilitates Platelet-derived Growth Factor-BB-stimulated ERK2 Activation Required for Chemotaxis Migration of Vascular Smooth Muscle Cells. J. Biol. Chem. 275: 41092-41099 [Abstract] [Full Text]
Dans, M., Gagnoux-Palacios, L., Blaikie, P., Klein, S., Mariotti, A., Giancotti, F. G. (2001). Tyrosine Phosphorylation of the beta 4 Integrin Cytoplasmic Domain Mediates Shc Signaling to Extracellular Signal-regulated Kinase and Antagonizes Formation of Hemidesmosomes. J. Biol. Chem. 276: 1494-1502 [Abstract] [Full Text]
Salazar, E. P., Rozengurt, E. (2001). Src Family Kinases Are Required for Integrin-mediated but Not for G Protein-coupled Receptor Stimulation of Focal Adhesion Kinase Autophosphorylation at Tyr-397. J. Biol. Chem. 276: 17788-17795 [Abstract] [Full Text]
Gu, J., Sumida, Y., Sanzen, N., Sekiguchi, K. (2001). Laminin-10/11 and Fibronectin Differentially Regulate Integrin- dependent Rho and Rac Activation via p130Cas-CrkII-DOCK180 Pathway. J. Biol. Chem. 276: 27090-27097 [Abstract] [Full Text]