Slk19p Is a Centromere Protein That Functions to Stabilize Mitotic Spindles

doi:10.1083/jcb.146.2.415

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J. Cell Biol., Volume 146, Number 2, July 26, 1999 415-426
Copyright © 1999 by The Rockefeller University Press.

Slk19p Is a Centromere Protein That Functions to Stabilize Mitotic Spindles

Xuemei Zeng^a, Jason A. Kahana^b, Pamela A. Silver^b, Mary K. Morphew^c, J. Richard McIntosh^c, Ian T. Fitch^d, John Carbon^d, and William S. Saunders^a
^a Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260
^b Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and The Dana Farber Cancer Institute, Boston, Massachusetts 02115
^c Department of Molecular, Cell, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347
^d Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, California 93106

Correspondence to: William S. Saunders, Department of Biological Sciences, 258 Crawford Hall, University of Pittsburgh, Pittsburgh, PA 15260., (412) 624-4320 (phone), (412) 624-4759 (fax)

We have identified a novel centromere-associated gene productfrom Saccharomyces cerevisiae that plays a role in spindle assemblyand stability. Strains with a deletion of SLK19 (synthetic lethalKar3p gene) exhibit abnormally short mitotic spindles, increasednumbers of astral microtubules, and require the presence ofthe kinesin motor Kar3p for viability. When cells are deprivedof both Slk19p and Kar3p, rapid spindle breakdown and mitoticarrest is observed. A functional fusion of Slk19p to green fluorescentprotein (GFP) localizes to kinetochores and, during anaphase,to the spindle midzone, whereas Kar3p-GFP was found at the nuclearside of the spindle pole body. Thus, these proteins seem toplay overlapping roles in stabilizing spindle structure whileacting from opposite ends of the microtubules.

Key Words: spindle, Saccharomyces cerevisiae, kinetochore, motor, yeast

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