Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 792K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ma, H. Articles by Berezney, R. Search for Related Content PubMed PubMed Citation Articles by Ma, H. Articles by Berezney, R. Related Collections Related Article Social Bookmarking                            What's this?

© The Rockefeller University Press, 0021-9525/1999//531 $5.00
The Journal of Cell Biology, Volume 146, Number 3, , 1999 531-542

Association of Chromosome Territories with the Nuclear Matrix

Hong Ma^a, Alan J. Siegel^a, and Ronald Berezney^a

^a Department of Biological Sciences, State University of New York at Buffalo, Buffalo, New York 14260
Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260.(716) 645-2975(716) 645-2874

berezney{at}acsu.buffalo.edu

To study the possible role of the nuclear matrix in chromosome territory organization, normal human fibroblast cells are treated in situ via classic isolation procedures for nuclear matrix in the absence of nuclease (e.g., DNase I) digestion, followed by chromosome painting. We report for the first time that chromosome territories are maintained intact on the nuclear matrix. In contrast, complete extraction of the internal nuclear matrix components with RNase treatment followed by 2 M NaCl results in the disruption of higher order chromosome territory architecture. Correlative with territorial disruption is the formation of a faint DNA halo surrounding the nuclear lamina and a dispersive effect on the characteristically discrete DNA replication sites in the nuclear interior. Identical results were obtained using eight different human chromosome paints. Based on these findings, we developed a fractionation strategy to release the bulk of nuclear matrix proteins under conditions where the chromosome territories are maintained intact. A second treatment results in disruption of the chromosome territories in conjunction with the release of a small subset of acidic proteins. These proteins are distinct from the major nuclear matrix proteins and may be involved in mediating chromosome territory organization.

Key Words: chromosome territory • chromosome painting • fluorescence in situ hybridization • DNA-rich nuclear matrix • nuclear matrix proteins

© 1999 The Rockefeller University Press

This article is dedicated to Professor Donald S. Coffey on the occasion of his 40th year at The Johns Hopkins University School of Medicine.

Dr. Ma's current address is Vollum Institute, L-474, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97201-3098.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Facebook

Reddit

Technorati

Twitter

This article has been cited by other articles:

• Takata, H., Nishijima, H., Ogura, S.-i., Sakaguchi, T., Bubulya, P. A., Mochizuki, T., Shibahara, K.-i. (2009). Proteome analysis of human nuclear insoluble fractions.. GENES CELLS 14: 975-990 [Abstract] [Full Text]  
• Linnemann, A. K., Platts, A. E., Krawetz, S. A. (2009). Differential nuclear scaffold/matrix attachment marks expressed genes. Hum Mol Genet 18: 645-654 [Abstract] [Full Text]  
• O'Gorman, W., Akoulitchev, A. (2006). What Is So Special About Oskar Wild?. Sci Signal 2006: pe51-pe51 [Abstract] [Full Text]  
• Mladenov, E., Anachkova, B., Tsaneva, I. (2006). Sub-nuclear localization of Rad51 in response to DNA damage.. GENES CELLS 11: 513-524 [Abstract] [Full Text]  
• Boutanaev, A. M., Mikhaylova, L. M., Nurminsky, D. I. (2005). The Pattern of Chromosome Folding in Interphase Is Outlined by the Linear Gene Density Profile. Mol. Cell. Biol. 25: 8379-8386 [Abstract] [Full Text]  
• Stein, G. S. (2005). Mechanogenomic Control of DNA Exposure and Sequestration. Am. J. Pathol. 166: 959-962 [Full Text]  
• Enukashvily, N., Donev, R., Sheer, D., Podgornaya, O. (2005). Satellite DNA binding and cellular localisation of RNA helicase P68. J. Cell Sci. 118: 611-622 [Abstract] [Full Text]  
• Martins, R. P., Ostermeier, G. C., Krawetz, S. A. (2004). Nuclear Matrix Interactions at the Human Protamine Domain: A WORKING MODEL OF POTENTIATION. J. Biol. Chem. 279: 51862-51868 [Abstract] [Full Text]  
• Zaidi, S. K., Young, D. W., Choi, J.-Y., Pratap, J., Javed, A., Montecino, M., Stein, J. L., Lian, J. B., van Wijnen, A. J., Stein, G. S. (2004). Intranuclear Trafficking: Organization and Assembly of Regulatory Machinery for Combinatorial Biological Control. J. Biol. Chem. 279: 43363-43366 [Abstract] [Full Text]  
• Iborra, F. J., Escargueil, A. E., Kwek, K. Y., Akoulitchev, A., Cook, P. R. (2004). Molecular cross-talk between the transcription, translation, and nonsense-mediated decay machineries. J. Cell Sci. 117: 899-906 [Abstract] [Full Text]  
• Sagara, J., Higuchi, T., Hattori, Y., Moriya, M., Sarvotham, H., Shima, H., Shirato, H., Kikuchi, K., Taniguchi, S. (2003). Scapinin, a Putative Protein Phosphatase-1 Regulatory Subunit Associated with the Nuclear Nonchromatin Structure. J. Biol. Chem. 278: 45611-45619 [Abstract] [Full Text]  
• Byrd, K., Corces, V. G. (2003). Visualization of chromatin domains created by the gypsy insulator of Drosophila. JCB 162: 565-574 [Abstract] [Full Text]  
• Mesner, L. D., Hamlin, J. L., Dijkwel, P. A. (2003). The matrix attachment region in the Chinese hamster dihydrofolate reductase origin of replication may be required for local chromatid separation. Proc. Natl. Acad. Sci. USA 100: 3281-3286 [Abstract] [Full Text]  
• Walter, J., Schermelleh, L., Cremer, M., Tashiro, S., Cremer, T. (2003). Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages. JCB 160: 685-697 [Abstract] [Full Text]  
• Donev, R., Horton, R., Beck, S., Doneva, T., Vatcheva, R., Bowen, W. R., Sheer, D. (2003). Recruitment of Heterogeneous Nuclear Ribonucleoprotein A1 in Vivo to the LMP/TAP Region of the Major Histocompatibility Complex. J. Biol. Chem. 278: 5214-5226 [Abstract] [Full Text]  
• Elias, M. C. Q. B., Faria, M., Mortara, R. A., Motta, M. C. M., de Souza, W., Thiry, M., Schenkman, S. (2002). Chromosome Localization Changes in the Trypanosoma cruzi Nucleus. Eukaryot Cell 1: 944-953 [Abstract] [Full Text]  
• Mauldin, S. K., Getts, R. C., Liu, W., Stamato, T. D. (2002). DNA-PK-dependent binding of DNA ends to plasmids containing nuclear matrix attachment region DNA sequences: evidence for assembly of a repair complex. Nucleic Acids Res 30: 4075-4087 [Abstract] [Full Text]  
• Kimura, Y., Sakai, F., Nakano, O., Kisaki, O., Sugimoto, H., Sawamura, T., Sadano, H., Osumi, T. (2002). The Newly Identified Human Nuclear Protein NXP-2 Possesses Three Distinct Domains, the Nuclear Matrix-binding, RNA-binding, and Coiled-coil Domains. J. Biol. Chem. 277: 20611-20617 [Abstract] [Full Text]  
• Spencer, V. A., Samuel, S. K., Davie, J. R. (2001). Altered Profiles in Nuclear Matrix Proteins Associated with DNA in Situ during Progression of Breast Cancer Cells. Cancer Res. 61: 1362-1366 [Abstract] [Full Text]  
• Nickerson, J (2001). Experimental observations of a nuclear matrix. J. Cell Sci. 114: 463-474 [Abstract]  
• Albagli, O., Lindon, C., Lantoine, D., Quief, S., Puvion, E., Pinset, C., Puvion-Dutilleul, F. (2000). DNA Replication Progresses on the Periphery of Nuclear Aggregates Formed by the BCL6 Transcription Factor. Mol. Cell. Biol. 20: 8560-8570 [Abstract] [Full Text]  
• Szentirmay, M. N., Sawadogo, M. (2000). SURVEY AND SUMMARY: Spatial organization of RNA polymerase II transcription in the nucleus. Nucleic Acids Res 28: 2019-2025 [Abstract] [Full Text]  
• Leonhardt, H., Rahn, H.-P., Weinzierl, P., Sporbert, A., Cremer, T., Zink, D., Cardoso, M. C. (2000). Dynamics of DNA Replication Factories in Living Cells. JCB 149: 271-280 [Abstract] [Full Text]  
• Berube, N. G., Smeenk, C. A., Picketts, D. J. (2000). Cell cycle-dependent phosphorylation of the ATRX protein correlates with changes in nuclear matrix and chromatin association. Hum Mol Genet 9: 539-547 [Abstract] [Full Text]  
• Stein, G., van Wijnen, A., Stein, J., Lian, J., Montecino, M, Choi, J, Zaidi, K, Javed, A (2000). Intranuclear trafficking of transcription factors: implications for biological control. J. Cell Sci. 113: 2527-2533 [Abstract]  
• Gerner, C., Frohwein, U., Gotzmann, J., Bayer, E., Gelbmann, D., Bursch, W., Schulte-Hermann, R. (2000). The Fas-induced Apoptosis Analyzed by High Throughput Proteome Analysis. J. Biol. Chem. 275: 39018-39026 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents