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© The Rockefeller University Press,
0021-9525/1999//531 $5.00
The Journal of Cell Biology, Volume 146, Number 3,
, 1999 531-542
Original Article |
Association of Chromosome Territories with the Nuclear Matrix
: Disruption of Human Chromosome Territories Correlates with the Release of a Subset of Nuclear Matrix Proteins
Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260.(716) 645-2975(716) 645-2874
berezney{at}acsu.buffalo.edu
To study the possible role of the nuclear matrix in chromosome territory organization, normal human fibroblast cells are treated in situ via classic isolation procedures for nuclear matrix in the absence of nuclease (e.g., DNase I) digestion, followed by chromosome painting. We report for the first time that chromosome territories are maintained intact on the nuclear matrix. In contrast, complete extraction of the internal nuclear matrix components with RNase treatment followed by 2 M NaCl results in the disruption of higher order chromosome territory architecture. Correlative with territorial disruption is the formation of a faint DNA halo surrounding the nuclear lamina and a dispersive effect on the characteristically discrete DNA replication sites in the nuclear interior. Identical results were obtained using eight different human chromosome paints. Based on these findings, we developed a fractionation strategy to release the bulk of nuclear matrix proteins under conditions where the chromosome territories are maintained intact. A second treatment results in disruption of the chromosome territories in conjunction with the release of a small subset of acidic proteins. These proteins are distinct from the major nuclear matrix proteins and may be involved in mediating chromosome territory organization.
Key Words: chromosome territory • chromosome painting • fluorescence in situ hybridization • DNA-rich nuclear matrix • nuclear matrix proteins
© 1999 The Rockefeller University Press
1.used in this paper: BrdU, 5-bromo-2-deoxy-uridine; CTAPs, chromosome territory anchor proteins; FISH, fluorescence in situ hybridization; MARs, matrix attachment regionsThis article is dedicated to Professor Donald S. Coffey on the occasion of his 40th year at The Johns Hopkins University School of Medicine.
Dr. Ma's current address is Vollum Institute, L-474, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97201-3098.
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