Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS) Analysis of the Lipid Molecular Species Composition of Yeast Subcellular Membranes Reveals Acyl Chain-based Sorting/Remodeling of Distinct Molecular Species En Route to the Plasma Membrane

doi:10.1083/jcb.146.4.741

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Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS) Analysis of the Lipid Molecular Species Composition of Yeast Subcellular Membranes Reveals Acyl Chain-based Sorting/Remodeling of Distinct Molecular Species En Route to the Plasma Membrane

Roger Schneiter^a, Britta Brügger^b, Roger Sandhoff^b, Günther Zellnig^c, Andrea Leber^a, Manfred Lampl^a, Karin Athenstaedt^a, Claudia Hrastnik^a, Sandra Eder^a, Günther Daum^a, Fritz Paltauf^a, Felix T. Wieland^b, and Sepp D. Kohlwein^a
^a Spezialforschungsbereich Biomembrane Research Center, Institut für Biochemie und Lebensmittelchemie, Technische Universität Graz, A-8010 Graz, Austria
^b Biochemie-Zentrum Heidelberg, Universität Heidelberg, D-69120 Heidelberg, Germany
^c Institut für Pflanzenphysiologie, Karl-Franzens Universität, A-8010 Graz, Austria

Correspondence to: Roger Schneiter, Institute of Biochemistry, Technical University Graz, Petersgasse 12, A-8010 Graz, Austria. Tel:43-316-873-6955 Fax:43-316-873-6952 E-mail:f548roge{at}mbox.tu-graz.ac.at.

Nano-electrospray ionization tandem mass spectrometry (nano-ESI-MS/MS)was employed to determine qualitative differences in the lipidmolecular species composition of a comprehensive set of organellarmembranes, isolated from a single culture of Saccharomyces cerevisiaecells. Remarkable differences in the acyl chain compositionof biosynthetically related phospholipid classes were observed.Acyl chain saturation was lowest in phosphatidylcholine (15.4%)and phosphatidylethanolamine (PE; 16.2%), followed by phosphatidylserine(PS; 29.4%), and highest in phosphatidylinositol (53.1%). Thelipid molecular species profiles of the various membranes weregenerally similar, with a deviation from a calculated averageprofile of ~± 20%. Nevertheless, clear distinctions betweenthe molecular species profiles of different membranes were observed,suggesting that lipid sorting mechanisms are operating at thelevel of individual molecular species to maintain the specificlipid composition of a given membrane. Most notably, the plasmamembrane is enriched in saturated species of PS and PE. Thenature of the sorting mechanism that determines the lipid compositionof the plasma membrane was investigated further. The accumulationof monounsaturated species of PS at the expense of diunsaturatedspecies in the plasma membrane of wild-type cells was reversedin elo3 ${Delta}$ mutant cells, which synthesize C24 fatty acid-substitutedsphingolipids instead of the normal C26 fatty acid-substitutedspecies. This observation suggests that acyl chain-based sortingand/or remodeling mechanisms are operating to maintain the specificlipid molecular species composition of the yeast plasma membrane.

Key Words: Saccharomyces cerevisiae, subcellular fractions, lipids, cellmembrane, mass fragmentography

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