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© The Rockefeller University Press, 0021-9525/1999//801 $5.00
The Journal of Cell Biology, Volume 146, Number 4, , 1999 801-818

Original Article

Domains in the 1{alpha} Dynein Heavy Chain Required for Inner Arm Assembly and Flagellar Motility in Chlamydomonas



Steven H. Mystera, Julie A. Knotta, Katrina M. Wysockia, Eileen O'Tooleb, and Mary E. Portera

a Department of Genetics, Cell Biology, and Development, University of Minnesota Medical School, Minneapolis, Minnesota 55455
b Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, Boulder, Colorado 80309-0347
Department of Genetics, Cell Biology, and Development, University of Minnesota Medical School, Box 206, 420 Delaware St. SE, 4-102 Owre Hall, Minneapolis, MN 55455.(612) 624-8118(612) 626-1901

mary-p{at}biosci.cbs.umn.edu

Flagellar motility is generated by the activity of multiple dynein motors, but the specific role of each dynein heavy chain (Dhc) is largely unknown, and the mechanism by which the different Dhcs are targeted to their unique locations is also poorly understood. We report here the complete nucleotide sequence of the Chlamydomonas Dhc1 gene and the corresponding deduced amino acid sequence of the 1{alpha} Dhc of the I1 inner dynein arm. The 1{alpha} Dhc is similar to other axonemal Dhcs, but two additional phosphate binding motifs (P-loops) have been identified in the NH2- and COOH-terminal regions. Because mutations in Dhc1 result in motility defects and loss of the I1 inner arm, a series of Dhc1 transgenes were used to rescue the mutant phenotypes. Motile cotransformants that express either full-length or truncated 1{alpha} Dhcs were recovered. The truncated 1{alpha} Dhc fragments lacked the dynein motor domain, but still assembled with the 1β Dhc and other I1 subunits into partially functional complexes at the correct axoneme location. Analysis of the transformants has identified the site of the 1{alpha} motor domain in the I1 structure and further revealed the role of the 1{alpha} Dhc in flagellar motility and phototactic behavior.

Key Words: motors • dynein • flagella • phototaxis • inner arm

© 1999 The Rockefeller University Press

used in this report: BAC, bacterial artificial chromosome; Dhc, dynein heavy chain; IC, intermediate chain; LC, light chain; OA, outer arm; P-loop, phosphate binding motif; P1-P4, Pn, Pc, P-loops 1-4, NH2- and COOH-terminal P-loop; RT-PCR, reverse transcriptase PCR


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In Brief
J. Cell Biol. 1999 146: 1-2. [Full Text] [PDF]



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