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© The Rockefeller University Press, 0021-9525/1999//1005 $5.00
The Journal of Cell Biology, Volume 146, Number 5, , 1999 1005-1018

mini spindles

C. Fiona Cullen^a, Peter Deák^b,c, David M. Glover^b,c, and Hiroyuki Ohkura^a,b

^a Institute of Cell and Molecular Biology, The University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
^b Cancer Research Campaign Laboratories, Department of Anatomy and Physiology, The University of Dundee, DD1 4HN, United Kingdom
^c Cancer Research Campaign Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom
Institute of Cell and Molecular Biology, The University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, Scotland, UK.+44-131-650-8650+44-131-650-7094

h.ohkura{at}ed.ac.uk

We describe a new Drosophila gene, mini spindles (msps) identified in a cytological screen for mitotic mutant. Mutation in msps disrupts the structural integrity of the mitotic spindle, resulting in the formation of one or more small additional spindles in diploid cells. Nucleation of microtubules from centrosomes, metaphase alignment of chromosomes, or the focusing of spindle poles appears much less affected. The msps gene encodes a 227-kD protein with high similarity to the vertebrate microtubule-associated proteins (MAPs), human TOGp and Xenopus XMAP215, and with limited similarity to the Dis1 and STU2 proteins from fission yeast and budding yeast. Consistent with their sequence similarity, Msps protein also associates with microtubules in vitro. In the embryonic division cycles, Msps protein localizes to centrosomal regions at all mitotic stages, and spreads over the spindles during metaphase and anaphase. The absence of centrosomal staining in interphase of the cellularized embryos suggests that the interactions between Msps protein and microtubules or centrosomes may be regulated during the cell cycle.

Key Words: spindle • microtubule • mitosis • cytoskeleton • Drosophila

© 1999 The Rockefeller University Press

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