JCB logo
amgmicro.com
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 2240K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, M.-g.
Right arrow Articles by Hays, T. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, M.-g.
Right arrow Articles by Hays, T. S.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*Protein
*UniGene
*Substance via MeSH
*Genetics Home Reference
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

© The Rockefeller University Press, 0021-9525/1999//1061 $5.00
The Journal of Cell Biology, Volume 146, Number 5, , 1999 1061-1074

Original Article

Filamin Is Required for Ring Canal Assembly and Actin Organization during Drosophila Oogenesis



Min-gang Lia, Madeline Serra, Kevin Edwardsb, Susan Ludmanna, Daisuke Yamamotoc, Lewis G. Tilneyd, Christine M. Fielde, and Thomas S. Haysa

a Department of Genetics, Cell and Developmental Biology, University of Minnesota, St. Paul, Minnesota 55108
b ERATO Yamamoto Behavior Genes Project, University of Hawaii, Honolulu, Hawaii 96822
c ERATO Yamamoto Behavior Genes Project, Waseda University School of Human Sciences, Tokorozawa, Saitama 359-1192, Japan
d Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104
e Department of Biology, Harvard Medical School, Boston, Massachusetts 02115
Department of Genetics, Cell and Developmental Biology, University of Minnesota, St. Paul, MN 55108.(612) 625-5754(612) 625-2226

tom-h{at}biosci.cbs.umn.edu

The remodeling of the actin cytoskeleton is essential for cell migration, cell division, and cell morphogenesis. Actin-binding proteins play a pivotal role in reorganizing the actin cytoskeleton in response to signals exchanged between cells. In consequence, actin-binding proteins are increasingly a focus of investigations into effectors of cell signaling and the coordination of cellular behaviors within developmental processes. One of the first actin-binding proteins identified was filamin, or actin-binding protein 280 (ABP280). Filamin is required for cell migration (Cunningham et al. 1992), and mutations in human {alpha}-filamin (FLN1; Fox et al. 1998) are responsible for impaired migration of cerebral neurons and give rise to periventricular heterotopia, a disorder that leads to epilepsy and vascular disorders, as well as embryonic lethality. We report the identification and characterization of a mutation in Drosophila filamin, the homologue of human {alpha}-filamin. During oogenesis, filamin is concentrated in the ring canal structures that fortify arrested cleavage furrows and establish cytoplasmic bridges between cells of the germline. The major structural features common to other filamins are conserved in Drosophila filamin. Mutations in Drosophila filamin disrupt actin filament organization and compromise membrane integrity during oocyte development, resulting in female sterility. The genetic and molecular characterization of Drosophila filamin provides the first genetic model system for the analysis of filamin function and regulation during development.

Key Words: filamin • ABP280 • Drosophila • actin • ring canals

© 1999 The Rockefeller University Press

1.used in this paper: ABP, actin-binding protein; EST, expressed sequence tag


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents