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© The Rockefeller University Press, 0021-9525/1999//993 $5.00
The Journal of Cell Biology, Volume 146, Number 5, , 1999 993-1004

-Synergin

: An Eh Domain–Containing Protein That Interacts with -Adaptin

Lesley J. Page^a, Penelope J. Sowerby^a, Winnie W.Y. Lui^a, and Margaret S. Robinson^a

^a Department of Clinical Biochemistry and Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 2XY, England
University of Cambridge, CIMR, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2XY, England.44-1223-76232244-1223-330163

msr12{at}mole.bio.cam.ac.uk

The AP-1 adaptor complex is associated with the TGN, where it links selected membrane proteins to the clathrin lattice, enabling these proteins to be concentrated in clathrin-coated vesicles. To identify other proteins that participate in the clathrin-coated vesicle cycle at the TGN, we have carried out a yeast two- hybrid library screen using the -adaptin subunit of the AP-1 complex as bait. Two novel, ubiquitously expressed proteins were found: p34, which interacts with both -adaptin and -adaptin, and -synergin, an alternatively spliced protein with an apparent molecular mass of 110–190 kD, which only interacts with -adaptin. -Synergin is associated with AP-1 both in the cytosol and on TGN membranes, and it is strongly enriched in clathrin-coated vesicles. It binds directly to the ear domain of -adaptin and it contains an Eps15 homology (EH) domain, although the EH domain is not part of the -adaptin binding site. In cells expressing -adaptin with the -adaptin ear, a construct that goes mainly to the plasma membrane, much of the -synergin is also rerouted to the plasma membrane, indicating that it follows AP-1 onto membranes rather than leading it there. The presence of an EH domain suggests that -synergin links the AP-1 complex to another protein or proteins.

Key Words: AP-1 • -adaptin • clathrin • TGN • EH domain

© 1999 The Rockefeller University Press

L.J. Page and P.J. Sowerby contributed equally to this work.

Dr. Page's present address is Imperial Cancer Research Fund, Lincoln's Inn Field, London WC2A 3PX.

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