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© The Rockefeller University Press,
0021-9525/1999//1211 $5.00
The Journal of Cell Biology, Volume 146, Number 6,
, 1999 1211-1226
Original Article |
Nuclear Organization of Mammalian Genomes
: Polar Chromosome Territories Build up Functionally Distinct Higher Order Compartments
b Stazione Zoologica Anton Dohrn, 80121 Napoli, Italy
c Institut für Anthropologie und Humangenetik, LMU München, 80333 München, Germany
d Chromatin and Gene Expression Group, Department of Anatomy, University of Birmingham Medical School, Birmingham B15 2TT, United Kingdom
Institut für Anthropologie und Humangenetik, LMU München, Goethestrasse 31, 80336 München, Germany.49-89-5996-61849-89-5996-617
dani.zink{at}lrz.uni-muenchen.de
We investigated the nuclear higher order compartmentalization of chromatin according to its replication timing (Ferreira et al. 1997) and the relations of this compartmentalization to chromosome structure and the spatial organization of transcription. Our aim was to provide a comprehensive and integrated view on the relations between chromosome structure and functional nuclear architecture. Using different mammalian cell types, we show that distinct higher order compartments whose DNA displays a specific replication timing are stably maintained during all interphase stages. The organizational principle is clonally inherited. We directly demonstrate the presence of polar chromosome territories that align to build up higher order compartments, as previously suggested (Ferreira et al. 1997). Polar chromosome territories display a specific orientation of early and late replicating subregions that correspond to R- or G/C-bands of mitotic chromosomes. Higher order compartments containing G/C-bands replicating during the second half of the S phase display no transcriptional activity detectable by BrUTP pulse labeling and show no evidence of transcriptional competence. Transcriptionally competent and active chromatin is confined to a coherent compartment within the nuclear interior that comprises early replicating R-band sequences. As a whole, the data provide an integrated view on chromosome structure, nuclear higher order compartmentalization, and their relation to the spatial organization of functional nuclear processes.
Key Words: nuclear architecture • genome organization • chromosome structure • nuclear compartments • polar chromosome territories
© 1999 The Rockefeller University Press
1.used in this paper: BrdU, bromodeoxyuridine; CldU, chlorodeoxyuridine; DAPI, 4',6-diamidino-2-phenylindole; HDF, human diploid fibroblast; IdU, iododeoxyuridine; SF, subchromosomal foci
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