Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 921K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rogers, S. L. Articles by Gelfand, V. I. Search for Related Content PubMed PubMed Citation Articles by Rogers, S. L. Articles by Gelfand, V. I. Related Collections Related Article Social Bookmarking                            What's this?

© The Rockefeller University Press, 0021-9525/1999//1265 $5.00
The Journal of Cell Biology, Volume 146, Number 6, , 1999 1265-1276

Regulation of Melanosome Movement in the Cell Cycle by Reversible Association with Myosin V

^a Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801
^b Institute of Protein Research, Russian Academy of Sciences, Poushchino, Russia 142292
^c Institut für Zellphysiologie und Biosystemtechnik, Mikroskopiezentrum, Universität Rostock, D-18055 Rostock, Germany
Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, B107 Chemical and Life Sciences Laboratory, 601 South Goodwin Avenue, Urbana, IL 61801.(217) 333-5982(217) 333-5972

vgelfand{at}uiuc.edu

Previously, we have shown that melanosomes of Xenopus laevis melanophores are transported along both microtubules and actin filaments in a coordinated manner, and that myosin V is bound to purified melanosomes (Rogers, S., and V.I. Gelfand. 1998. Curr. Biol. 8:161–164). In the present study, we have demonstrated that myosin V is the actin-based motor responsible for melanosome transport. To examine whether myosin V was regulated in a cell cycle-dependent manner, purified melanosomes were treated with interphase- or metaphase-arrested Xenopus egg extracts and assayed for in vitro motility along Nitella actin filaments. Motility of organelles treated with mitotic extract was found to decrease dramatically, as compared with untreated or interphase extract-treated melanosomes. This mitotic inhibition of motility correlated with the dissociation of myosin V from melanosomes, but the activity of soluble motor remained unaffected. Furthermore, we find that myosin V heavy chain is highly phosphorylated in metaphase extracts versus interphase extracts. We conclude that organelle transport by myosin V is controlled by a cell cycle-regulated association of this motor to organelles, and that this binding is likely regulated by phosphorylation of myosin V during mitosis.

Key Words: myosin • molecular motors • melanophores • melanosomes • regulation

© 1999 The Rockefeller University Press

CiteULike

Complore

Connotea

Del.icio.us

Digg

Facebook

Reddit

Technorati

Twitter

This article has been cited by other articles:

• Lecuona, E., Minin, A., Trejo, H. E., Chen, J., Comellas, A. P., Sun, H., Grillo, D., Nekrasova, O. E., Welch, L. C., Szleifer, I., Gelfand, V. I., Sznajder, J. I. (2009). Myosin-Va restrains the trafficking of Na+/K+-ATPase-containing vesicles in alveolar epithelial cells. J. Cell Sci. 122: 3915-3922 [Abstract] [Full Text]  
• Boyle, R. T., McNamara, J. C. (2008). A Spring-Matrix Model for Pigment Translocation in the Red Ovarian Chromatophores of the Freshwater Shrimp Macrobrachium olfersi (Crustacea, Decapoda). Biol. Bull. 214: 111-121 [Abstract] [Full Text]  
• Yu, H.-Y. E., Bement, W. M. (2007). Multiple Myosins Are Required to Coordinate Actin Assembly with Coat Compression during Compensatory Endocytosis. Mol. Biol. Cell 18: 4096-4105 [Abstract] [Full Text]  
• Martel, J. A., Michael, D., Fejes-Toth, G., Naray-Fejes-Toth, A. (2007). Melanophilin, a novel aldosterone-induced gene in mouse cortical collecting duct cells. Am. J. Physiol. Renal Physiol. 293: F904-F913 [Abstract] [Full Text]  
• Li, B. X., Satoh, A. K., Ready, D. F. (2007). Myosin V, Rab11, and dRip11 direct apical secretion and cellular morphogenesis in developing Drosophila photoreceptors. JCB 177: 659-669 [Abstract] [Full Text]  
• Sato, O., Li, X.-d., Ikebe, M. (2007). Myosin Va Becomes a Low Duty Ratio Motor in the Inhibited Form. J. Biol. Chem. 282: 13228-13239 [Abstract] [Full Text]  
• Matson, S., Markoulaki, S., Ducibella, T. (2006). Antagonists of Myosin Light Chain Kinase and of Myosin II Inhibit Specific Events of Egg Activation in Fertilized Mouse Eggs. Biol. Reprod. 74: 169-176 [Abstract] [Full Text]  
• Homma, K., Ikebe, M. (2005). Myosin X Is a High Duty Ratio Motor. J. Biol. Chem. 280: 29381-29391 [Abstract] [Full Text]  
• Pashkova, N., Catlett, N. L., Novak, J. L., Weisman, L. S. (2005). A Point Mutation in the Cargo-Binding Domain of Myosin V Affects Its Interaction with Multiple Cargoes. Eukaryot Cell 4: 787-798 [Abstract] [Full Text]  
• Mitin, N. Y., Ramocki, M. B., Zullo, A. J., Der, C. J., Konieczny, S. F., Taparowsky, E. J. (2004). Identification and Characterization of Rain, a Novel Ras-interacting Protein with a Unique Subcellular Localization. J. Biol. Chem. 279: 22353-22361 [Abstract] [Full Text]  
• Krementsov, D. N., Krementsova, E. B., Trybus, K. M. (2004). Myosin V: regulation by calcium, calmodulin, and the tail domain. JCB 164: 877-886 [Abstract] [Full Text]  
• Cao, T. T., Chang, W., Masters, S. E., Mooseker, M. S. (2004). Myosin-Va Binds to and Mechanochemically Couples Microtubules to Actin Filaments. Mol. Biol. Cell 15: 151-161 [Abstract] [Full Text]  
• Wollert, T., Weiss, D. G., Gerdes, H.-H., Kuznetsov, S. A. (2002). Activation of myosin V-based motility and F-actin-dependent network formation of endoplasmic reticulum during mitosis. JCB 159: 571-577 [Abstract] [Full Text]  
• Wu, X., Wang, F., Rao, K., Sellers, J. R., Hammer, J. A. III (2002). Rab27a Is an Essential Component of Melanosome Receptor for Myosin Va. Mol. Biol. Cell 13: 1735-1749 [Abstract] [Full Text]  
• Gross, S. P., Tuma, M. C., Deacon, S. W., Serpinskaya, A. S., Reilein, A. R., Gelfand, V. I. (2002). Interactions and regulation of molecular motors in Xenopus melanophores. JCB 156: 855-865 [Abstract] [Full Text]  
• Rodriguez, O. C., Cheney, R. E. (2002). Human myosin-Vc is a novel class V myosin expressed in epithelial cells. J. Cell Sci. 115: 991-1004 [Abstract] [Full Text]  
• Karcher, R. L., Roland, J. T., Zappacosta, F., Huddleston, M. J., Annan, R. S., Carr, S. A., Gelfand, V. I. (2001). Cell Cycle Regulation of Myosin-V by Calcium/Calmodulin-Dependent Protein Kinase II. Science 293: 1317-1320 [Abstract] [Full Text]  
• Reese, E. L., Haimo, L. T. (2000). Dynein, Dynactin, and Kinesin II's Interaction with Microtubules Is Regulated during Bidirectional Organelle Transport. JCB 151: 155-166 [Abstract] [Full Text]  
• Catlett, N. L., Duex, J. E., Tang, F., Weisman, L. S. (2000). Two Distinct Regions in a Yeast Myosin-V Tail Domain Are Required for the Movement of Different Cargoes. JCB 150: 513-526 [Abstract] [Full Text]  
• Wade, N., Bryant, N. J., Connolly, L. M., Simpson, R. J., Luzio, J. P., Piper, R. C., James, D. E. (2001). Syntaxin 7 Complexes with Mouse Vps10p Tail Interactor 1b, Syntaxin 6, Vesicle-associated Membrane Protein (VAMP)8, and VAMP7 in B16 Melanoma Cells. J. Biol. Chem. 276: 19820-19827 [Abstract] [Full Text]  
• Addinall, S. G., Mayr, P. S., Doyle, S., Sheehan, J. K., Woodman, P. G., Allan, V. J. (2001). Phosphorylation by cdc2-CyclinB1 Kinase Releases Cytoplasmic Dynein from Membranes. J. Biol. Chem. 276: 15939-15944 [Abstract] [Full Text]  
• Stachelek, S. J., Tuft, R. A., Lifschitz, L. M., Leonard, D. M., Farwell, A. P., Leonard, J. L. (2001). Real-time Visualization of Processive Myosin 5a-mediated Vesicle Movement in Living Astrocytes. J. Biol. Chem. 276: 35652-35659 [Abstract] [Full Text]  
• Gross, S. P., Tuma, M. C., Deacon, S. W., Serpinskaya, A. S., Reilein, A. R., Gelfand, V. I. (2002). Interactions and regulation of molecular motors in Xenopus melanophores. JCB 156: 855-865 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents