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© The Rockefeller University Press, 0021-9525/1999//207 $5.00
The Journal of Cell Biology, Volume 147, Number 2, , 1999 207-220

Original Article

Rad51 and Dmc1 Form Mixed Complexes Associated with Mouse Meiotic Chromosome Cores and Synaptonemal Complexes



Madalena Tarsounasa, Takashi Moritab, Ronald E. Pearlmana, and Peter B. Moensa

a Department of Biology, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3, Canada
b Department of Molecular Genetics, Osaka City University Medical School, 1-4-3, Asahimachi, Abeno-ku, Osaka 545-8585, Japan
Department of Biology, York University, 4700 Keele Street, Toronto, ON, M3J 1P3 Canada.(416) 736-5731(416) 736-5358

moens{at}yorku.ca

The eukaryotic RecA homologues RAD51 and DMC1 function in homology recognition and formation of joint-molecule recombination intermediates during yeast meiosis. The precise immunolocalization of these two proteins on the meiotic chromosomes of plants and animals has been complicated by their high degree of identity at the amino acid level. With antibodies that have been immunodepleted of cross-reactive epitopes, we demonstrate that RAD51 and DMC1 have identical distribution patterns in extracts of mouse spermatocytes in successive prophase I stages, suggesting coordinate functionality. Immunofluorescence and immunoelectron microscopy with these antibodies demonstrate colocalization of the two proteins on the meiotic chromosome cores at early prophase I. We also show that mouse RAD51 and DMC1 establish protein–protein interactions with each other and with the chromosome core component COR1(SCP3) in a two-hybrid system and in vitro binding analyses. These results suggest that the formation of a multiprotein recombination complex associated with the meiotic chromosome cores is essential for the development and fulfillment of the meiotic recombination process.

Key Words: meiosis • genetic recombination • RAD51 • DMC1 • synaptonemal complex

© 1999 The Rockefeller University Press

1.used in this paper: DSBs, double strand breaks; SC, synaptonemal complex; ssDNA, single-stranded DNA

Dr. Tarsounas' present address is Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, EN6 3LD, UK.


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