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© The Rockefeller University Press, 0021-9525/1999//659 $5.00
The Journal of Cell Biology, Volume 147, Number 3, , 1999 659-670

Original Article

Cloning and Characterization of a Potassium-Dependent Sodium/Calcium Exchanger in Drosophila



K. Haug-Colleta, B. Pearsona, R. Webela, R.T. Szerencseib, R.J. Winkfeinb, P.P.M. Schnetkampb, and N.J. Colleya

a Department of Ophthalmology and Visual Sciences, Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706
b Department of Physiology and Biophysics, Medical Research Council Group on Ion Channels and Transporters, University of Calgary, Faculty of Medicine, Calgary, Alberta T2N 4N1, Canada
Department of Ophthalmology and Visual Sciences, Department of Genetics, University of Wisconsin, Madison, WI 53706.(608) 265-6021(608) 265-5398

njcolley{at}facstaff.wisc.edu

Sodium/calcium(-potassium) exchangers (NCX and NCKX) are critical for the rapid extrusion of calcium, which follows the stimulation of a variety of excitable cells. To further understand the mechanisms of calcium regulation in signaling, we have cloned a Drosophila sodium/calcium-potassium exchanger, Nckx30C. The overall deduced protein topology for NCKX30C is similar to that of mammalian NCKX, having five membrane-spanning domains in the NH2 terminus separated from six at the COOH-terminal end by a large intracellular loop. We show that NCKX30C functions as a potassium-dependent sodium/calcium exchanger, and is not only expressed in adult neurons as was expected, but is also expressed during ventral nerve cord development in the embryo and in larval imaginal discs. Nckx30C is expressed in a dorsal–ventral pattern in the eye-antennal disc in a pattern that is similar to, but broader than that of wingless, suggesting that large fluxes of calcium may be occurring during imaginal disc development. Nckx30C may not only function in the removal of calcium and maintenance of calcium homeostasis during signaling in the adult, but may also play a critical role in signaling during development.

Key Words: calcium • development • Drosophila • photoreceptor • signal transduction

© 1999 The Rockefeller University Press

1.used in this paper: NCKX, potassium-dependent sodium/calcium exchanger(s); NCX, sodium/calcium exchanger(s); SSC, saline sodium citrate; TM, transmembrane


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