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© The Rockefeller University Press, 0021-9525/1999/11/775/ $5.00
The Journal of Cell Biology, Volume 147, Number 4, November 15, 1999 775-790

Original Article

Cathepsin S Controls the Trafficking and Maturation of MHC Class II Molecules in Dendritic Cells

Christoph Driessena, Rebecca A.R. Bryanta, Ana-Maria Lennon-Duménila, José A. Villadangosa, Paula Wolf Bryanta, Guo-Ping Shib, Harold A. Chapmanb, and Hidde L. Ploegha
a Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115
b Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115

Correspondence to: Hidde L. Ploegh, Department of Pathology, Harvard Medical School, Building D2, 200 Longwood Ave., Boston, MA 02115. Tel:(617) 432-4776 Fax:(617) 432-4775 E-mail:ploegh{at}mit.edu.

Before a class II molecule can be loaded with antigenic material and reach the surface to engage CD4+ T cells, its chaperone, the class II-associated invariant chain (Ii), is degraded in a stepwise fashion by proteases in endocytic compartments. We have dissected the role of cathepsin S (CatS) in the trafficking and maturation of class II molecules by combining the use of dendritic cells (DC) from CatS-/- mice with a new active site–directed probe for direct visualization of active CatS. Our data demonstrate that CatS is active along the entire endocytic route, and that cleavage of the lysosomal sorting signal of Ii by CatS can occur there in mature DC. Genetic disruption of CatS dramatically reduces the flow of class II molecules to the cell surface. In CatS-/- DC, the bulk of major histocompatibility complex (MHC) class II molecules is retained in late endocytic compartments, although paradoxically, surface expression of class II is largely unaffected. The greatly diminished but continuous flow of class II molecules to the cell surface, in conjunction with their long half-life, can account for the latter observation. We conclude that in DC, CatS is a major determinant in the regulation of intracellular trafficking of MHC class II molecules.

Key Words: major histocompatibility complex class II, cathepsins, dendritic cells, antigen presentation, biological transport


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