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© The Rockefeller University Press, 0021-9525/1999//1023 $5.00
The Journal of Cell Biology, Volume 147, Number 5, , 1999 1023-1038

Original Article

Phosphorylation of Myosin-Binding Subunit (Mbs) of Myosin Phosphatase by Rho-Kinase in Vivo



Yoji Kawanoa, Yuko Fukataa, Noriko Oshiroa, Mutsuki Amanoa, Toshikazu Nakamurab, Masaaki Itoc, Fumio Matsumurad, Masaki Inagakie, and Kozo Kaibuchia

a Division of Signal Transduction, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan
b Division of Biochemistry, Osaka University Medical School, Suita, Osaka 565-0871, Japan
c The First Department of Internal Medicine, Mie University School of Medicine, Edobashi, Tsu, Mie 514-8507, Japan
d Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08855
e Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya 464-0021, Japan
Division of Signal Transduction, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan.81-743-72-544981-743-72-5440

kaibuchi{at}bs.aist-nara.ac.jp

Rho-associated kinase (Rho-kinase), which is activated by the small GTPase Rho, phosphorylates myosin-binding subunit (MBS) of myosin phosphatase and thereby inactivates the phosphatase activity in vitro. Rho-kinase is thought to regulate the phosphorylation state of the substrates including myosin light chain (MLC), ERM (ezrin/radixin/moesin) family proteins and adducin by their direct phosphorylation and by the inactivation of myosin phosphatase. Here we identified the sites of phosphorylation of MBS by Rho-kinase as Thr-697, Ser-854 and several residues, and prepared antibody that specifically recognized MBS phosphorylated at Ser-854. We found by use of this antibody that the stimulation of MDCK epithelial cells with tetradecanoylphorbol-13-acetate (TPA) or hepatocyte growth factor (HGF) induced the phosphorylation of MBS at Ser-854 under the conditions in which membrane ruffling and cell migration were induced. Pretreatment of the cells with Botulinum C3 ADP-ribosyltransferase (C3), which is thought to interfere with Rho functions, or Rho-kinase inhibitors inhibited the TPA- or HGF-induced MBS phosphorylation. The TPA stimulation enhanced the immunoreactivity of phosphorylated MBS in the cytoplasm and membrane ruffling area of MDCK cells. In migrating MDCK cells, phosphorylated MBS as well as phosphorylated MLC at Ser-19 were localized in the leading edge and posterior region. Phosphorylated MBS was localized on actin stress fibers in REF52 fibroblasts. The microinjection of C3 or dominant negative Rho-kinase disrupted stress fibers and weakened the accumulation of phosphorylated MBS in REF52 cells. During cytokinesis, phosphorylated MBS, MLC and ERM family proteins accumulated at the cleavage furrow, and the phosphorylation level of MBS at Ser-854 was increased. Taken together, these results indicate that MBS is phosphorylated by Rho-kinase downstream of Rho in vivo, and suggest that myosin phosphatase and Rho-kinase spatiotemporally regulate the phosphorylation state of Rho-kinase substrates including MLC and ERM family proteins in vivo in a cooperative manner.

Key Words: myosin-binding subunit of myosin phosphatase • Rho-associated kinase • Rho • phosphorylation • cytokinesis

© 1999 The Rockefeller University Press

Abbreviations used in this paper: aa, amino acids; C3, Botulinum C3 ADP-ribosyltransferase; CAT, the catalytic domain of Rho-kinase; dibutyryl cAMP, N6,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate; ERM family proteins, ezrin/radixin/moesin; GST, glutathione-S-transferase; GTP{gamma}S, guanosine 5'-(3-O-thio)triphosphate; HGF, hepatocyte growth factor; MBP, maltose-binding protein; MBS, myosin-binding subunit; MLC, myosin light chain; PKC, protein kinase C; Rho-kinase, Rho-associated kinase; TPA, tetradecanoylphorbol-13-acetate; TRITC, tetramethylrhodamine B isothiocyanate.


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