The TOM Core Complex: The General Protein Import Pore of the Outer Membrane of Mitochondria

doi:10.1083/jcb.147.5.959

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© The Rockefeller University Press, 0021-9525/1999/11/959/ $5.00
The Journal of Cell Biology, Volume 147, Number 5, November 29, 1999 959-968

Original Article

The TOM Core Complex: The General Protein Import Pore of the Outer Membrane of Mitochondria

Uwe Ahting^a, Clemens Thun^a, Reiner Hegerl^b, Dieter Typke^b, Frank E. Nargang^c, Walter Neupert^a, and Stephan Nussberger^a
^a Institut für Physiologische Chemie der Universität München, D-80336 München, Germany
^b Abteilung für Molekulare Strukturbiologie, Max-Planck Institut für Biochemie, D-82152 Martinsried, Germany
^c Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada

Correspondence to: Walter Neupert, Institut für Physiologische Chemie der Universität München, Goethestraße 33, D-80336 München, Germany. Tel:49 (89) 5996 312

Translocation of nuclear-encoded preproteins across the outermembrane of mitochondria is mediated by the multicomponent transmembraneTOM complex. We have isolated the TOM core complex of Neurosporacrassa by removing the receptors Tom70 and Tom20 from the isolatedTOM holo complex by treatment with the detergent dodecyl maltoside.It consists of Tom40, Tom22, and the small Tom components, Tom6and Tom7. This core complex was also purified directly frommitochondria after solubilization with dodecyl maltoside. TheTOM core complex has the characteristics of the general insertionpore; it contains high-conductance channels and binds preproteinin a targeting sequence-dependent manner. It forms a doublering structure that, in contrast to the holo complex, lacksthe third density seen in the latter particles. Three-dimensionalreconstruction by electron tomography exhibits two open porestraversing the complex with a diameter of $~$ 2.1 nm and a heightof $~$ 7 nm. Tom40 is the key structural element of the TOM corecomplex.

Key Words: TOM complex, mitochondria, protein translocation channel, electrontomography, protein targeting

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