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© The Rockefeller University Press, 0021-9525/1999//1137 $5.00
The Journal of Cell Biology, Volume 147, Number 6, , 1999 1137-1144

Brief Report

Mutations in the {alpha}-Tubulin 67C Gene Specifically Impair Achiasmate Segregation in Drosophila melanogaster



Heinrich J.G. Matthiesa, Lisa G. Messinaa, Ruria Nambaa, Kimberly J. Greera, M.Y. Walkera, and R. Scott Hawleya

a Department of Genetics, Section of Molecular and Cellular Biology, University of California at Davis, Davis, California 95616
Department of Genetics, Section of Molecular and Cellular Biology, University of California at Davis, Davis, California 95616.(530) 752-1185(530) 752-5146

shawley{at}netcom.com

Drosophila melanogaster oocytes heterozygous for mutations in the {alpha}-tubulin 67C gene ({alpha}tub67C) display defects in centromere positioning during prometaphase of meiosis I. The centromeres do not migrate to the poleward edges of the chromatin mass, and the chromatin fails to stretch during spindle lengthening. These results suggest that the poleward forces acting at the kinetochore are compromised in the {alpha}tub67C mutants. Genetic studies demonstrate that these mutations also strongly and specifically decrease the fidelity of achiasmate chromosome segregation. Proper centromere orientation, chromatin elongation, and faithful segregation can all be restored by a decrease in the amount of the Nod chromokinesin. These results suggest that the accurate segregation of achiasmate chromosomes requires the proper balancing of forces acting on the chromosomes during prometaphase.

Key Words: chromosome • kinesin • meiosis • centromeres • meiotic spindle

© 1999 The Rockefeller University Press

Heinrich J.G. Matthies and Lisa G. Messina contributed equally to this manuscript.

Abbreviation used in this paper: {alpha}tub67C, {alpha}-tubulin 67C gene.


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J. Cell Biol. 1999 147: 1-2. [Full Text] [PDF]





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