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© The Rockefeller University Press, 0021-9525/1999//1145 $5.00
The Journal of Cell Biology, Volume 147, Number 6, , 1999 1145-1152

Brief Report

Axo-Glial Interactions Regulate the Localization of Axonal Paranodal Proteins



Jeffrey L. Dupreea, Jean-Antoine Giraultd, and Brian Popkoa,b,c

a Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
b Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
c Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
d Institut National de la Santé et la Recherche Médicale, Unit 114, Collège de France, Paris 75231, France
Neuroscience Center, CB#7250, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7250.(919) 966-9605(919) 966-1449

popko{at}css.unc.edu

Mice incapable of synthesizing the abundant galactolipids of myelin exhibit disrupted paranodal axo-glial interactions in the central and peripheral nervous systems. Using these mutants, we have analyzed the role that axo-glial interactions play in the establishment of axonal protein distribution in the region of the node of Ranvier. Whereas the clustering of the nodal proteins, sodium channels, ankyrinG, and neurofascin was only slightly affected, the distribution of potassium channels and paranodin, proteins that are normally concentrated in the regions juxtaposed to the node, was dramatically altered. The potassium channels, which are normally concentrated in the paranode/juxtaparanode, were not restricted to this region but were detected throughout the internode in the galactolipid-defi- cient mice. Paranodin/contactin-associated protein (Caspr), a paranodal protein that is a potential neuronal mediator of axon-myelin binding, was not concentrated in the paranodal regions but was diffusely distributed along the internodal regions. Collectively, these findings suggest that the myelin galactolipids are essential for the proper formation of axo-glial interactions and demonstrate that a disruption in these interactions results in profound abnormalities in the molecular organization of the paranodal axolemma.

Key Words: paranodin • potassium channels • sodium channels • galactolipids • axo-glial interactions

© 1999 The Rockefeller University Press

Abbreviations used in this paper: Caspr, contactin-associated protein; CGT, ceramide galactosyltransferase; CNS, central nervous system; GalC, galactocerebroside; PB, phosphate buffer; PNS, peripheral nervous system.


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