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© The Rockefeller University Press, 0021-9525/1999//1223 $5.00
The Journal of Cell Biology, Volume 147, Number 6, , 1999 1223-1236

Original Article

Role for Drs2p, a P-Type Atpase and Potential Aminophospholipid Translocase, in Yeast Late Golgi Function



Chih-Ying Chena, Michael F. Ingrama, Peter H. Rosala, and Todd R. Grahama

a Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235
Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235.(615) 343-6707(615) 343-1835

tr.graham{at}vanderbilt.edu

ADP-ribosylation factor appears to regulate the budding of both COPI and clathrin-coated transport vesicles from Golgi membranes. An arf1{Delta} synthetic lethal screen identified SWA3/DRS2, which encodes an integral membrane P-type ATPase and potential aminophospholipid translocase (or flippase). The drs2 null allele is also synthetically lethal with clathrin heavy chain (chc1) temperature-sensitive alleles, but not with mutations in COPI subunits or other SEC genes tested. Consistent with these genetic analyses, we found that the drs2{Delta} mutant exhibits late Golgi defects that may result from a loss of clathrin function at this compartment. These include a defect in the Kex2-dependent processing of pro–{alpha}-factor and the accumulation of abnormal Golgi cisternae. Moreover, we observed a marked reduction in clathrin-coated vesicles that can be isolated from the drs2{Delta} cells. Subcellular fractionation and immunofluorescence analysis indicate that Drs2p localizes to late Golgi membranes containing Kex2p. These observations indicate a novel role for a P-type ATPase in late Golgi function and suggest a possible link between membrane asymmetry and clathrin function at the Golgi complex.

Key Words: adenosine diphosphate–ribosylation factor • aminophospholipid translocase • clathrin • DRS2 • trans-Golgi network

© 1999 The Rockefeller University Press

Drs. Chen and Ingram contributed equally to this work and should be considered co–first authors.

Abbreviations used in this paper: AP, adaptor protein; ARF, ADP-ribosylation factor; CCV, clathrin-coated vesicle; CPY, carboxypeptidase Y; EM, electron microscopy; HA, hemagglutinin; ORF, open reading frame; PE, phosphatidylethanolamine; PS, phosphatidylserine; ts allele, temperature-sensitive allele.


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J. Cell Biol. 1999 147: 1-2. [Full Text] [PDF]



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