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© The Rockefeller University Press, 0021-9525/1999/12/1237/ $5.00
The Journal of Cell Biology, Volume 147, Number 6, December 13, 1999 1237-1248

Original Article

Intracellular Trafficking of Variant Chicken Kidney AE1 Anion Exchangers: Role of Alternative NH2 Termini in Polarized Sorting and Golgi Recycling

Tracy L. Adair-Kirka, Kathleen H. Coxa, and John V. Coxa
a Department of Microbiology and Immunology, University of Tennessee Health Science Center, 858 Madison Avenue, Memphis, Tennessee 38163

Correspondence to: John V. Cox, Department of Microbiology and Immunology, University of Tennessee, 858 Madison Avenue, Memphis, TN 38163. Tel:(901) 448-7080 Fax:(901) 448-8462 E-mail:jcox{at}utmem1.utmem.edu.

The variant chicken kidney AE1 anion exchangers differ only at the NH2 terminus of their cytoplasmic domains. Transfection studies have indicated that the variant chicken AE1-4 anion exchanger accumulates in the basolateral membrane of polarized MDCK kidney epithelial cells, while the AE1-3 variant, which lacks the NH2-terminal 63 amino acids of AE1-4, primarily accumulates in the apical membrane. Mutagenesis studies have shown that the basolateral accumulation of AE1-4 is dependent upon two tyrosine residues at amino acids 44 and 47 of the polypeptide. Interestingly, either of these tyrosines is sufficient to direct efficient basolateral sorting of AE1-4. However, in the absence of both tyrosine residues, AE1-4 accumulates in the apical membrane of MDCK cells. Pulse–chase studies have shown that after delivery to the cell surface, newly synthesized AE1-4 is recycled to the Golgi where it acquires additional N-linked sugar modifications. This Golgi recycling activity is dependent upon the same cytoplasmic tyrosine residues that are required for the basolateral sorting of this variant transporter. Furthermore, mutants of AE1-4 that are defective in Golgi recycling are unable to associate with the detergent insoluble actin cytoskeleton and are rapidly turned over. These studies, which represent the first description of tyrosine-dependent cytoplasmic sorting signal for a type III membrane protein, have suggested a critical role for the actin cytoskeleton in regulating AE1 anion exchanger localization and stability in this epithelial cell type.

Key Words: epithelial, band 3 protein, Golgi, polarity, actin


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