A Novel Muscle-Specific {beta}1 Integrin Binding Protein (Mibp) That Modulates Myogenic Differentiation

doi:10.1083/jcb.147.7.1391

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© The Rockefeller University Press, 0021-9525/1999//1391 $5.00
The Journal of Cell Biology, Volume 147, Number 7, , 1999 1391-1398

Brief Report

A Novel Muscle-Specific β1 Integrin Binding Protein (Mibp) That Modulates Myogenic Differentiation

Ji Li^a, Richard Mayne^a, and Chuanyue Wu^a

^a Department of Cell Biology and The Cell Adhesion and Matrix Research Center, University of Alabama at Birmingham, Birmingham, Alabama 35294-0019
VH 217, Department of Cell Biology, University of Alabama at Birmingham, 1670 University Blvd., Birmingham, AL 35294-0019.(205) 934-7029(205) 975-2253

cwu{at}cellbio.bhs.uab.edu

Myogenesis is regulated by cell adhesion receptors, includingintegrins of the β1 family. We report the identificationof a novel muscle-specific β1 integrin binding protein(MIBP). MIBP binds to the membrane-proximal cytoplasmic regionshared by β1A and β1D integrins, and the binding occursin vivo as well as in vitro. Furthermore, we show that MIBPis abundantly expressed by C2C12 myogenic cells before fusion,and the expression of MIBP is dramatically downregulated duringsubsequent differentiation. Finally, we show that overexpressionof MIBP in C2C12 cells resulted in a suppression of fusion andterminal differentiation, suggesting that MIBP may play a keyrole in controlling the progression of muscle differentiation.

Key Words: integrin binding protein • muscle • myogenic differentiation • signal transduction • myogenin

Abbreviations used in this paper: GST, glutathione S-transferase;MBP, maltose binding protein; MIBP, muscle integrin bindingprotein.

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