Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 348K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mermoud, J. E. Articles by Brockdorff, N. Search for Related Content PubMed PubMed Citation Articles by Mermoud, J. E. Articles by Brockdorff, N. Social Bookmarking                            What's this?

© The Rockefeller University Press, 0021-9525/1999//1399 $5.00
The Journal of Cell Biology, Volume 147, Number 7, , 1999 1399-1408

Histone Macroh2a1.2 Relocates to the Inactive X Chromosome after Initiation and Propagation of X-Inactivation

^a X-Inactivation Group, Medical Research Council Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London W12 0NN, United Kingdom
^b Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104
MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Rd., London W12 0NN, United Kingdom.44-181-383-830344-181-383-8278

jmermoud{at}hgmp.mrc.ac.uk

The histone macroH2A1.2 has been implicated in X chromosome inactivation on the basis of its accumulation on the inactive X chromosome (Xi) of adult female mammals. We have established the timing of macroH2A1.2 association with the Xi relative to the onset of X-inactivation in differentiating murine embryonic stem (ES) cells using immuno-RNA fluorescence in situ hybridization (FISH). Before X-inactivation we observe a single macroH2A1.2-dense region in both undifferentiated XX and XY ES cells that does not colocalize with X inactive specific transcript (Xist) RNA, and thus appears not to associate with the X chromosome(s). This pattern persists through early stages of differentiation, up to day 7. Then the frequency of XY cells containing a macroH2A1.2-rich domain declines. In contrast, in XX cells there is a striking relocalization of macroH2A1.2 to the Xi. Relocalization occurs in a highly synchronized wave over a 2-d period, indicating a precisely regulated association. The timing of macroH2A1.2 accumulation on the Xi suggests it is not necessary for the initiation or propagation of random X-inactivation.

Key Words: histones • X inactive specific transcript (Xist) RNA • X chromosome inactivation • differentiation • nuclear structure

© 1999 The Rockefeller University Press

CiteULike

Complore

Connotea

Del.icio.us

Digg

Facebook

Reddit

Technorati

Twitter

This article has been cited by other articles:

• Mietton, F., Sengupta, A. K., Molla, A., Picchi, G., Barral, S., Heliot, L., Grange, T., Wutz, A., Dimitrov, S. (2009). Weak but Uniform Enrichment of the Histone Variant macroH2A1 along the Inactive X Chromosome. Mol. Cell. Biol. 29: 150-156 [Abstract] [Full Text]  
• Schneider, R., Grosschedl, R. (2007). Dynamics and interplay of nuclear architecture, genome organization, and gene expression. Genes Dev. 21: 3027-3043 [Abstract] [Full Text]  
• Chaumeil, J., Le Baccon, P., Wutz, A., Heard, E. (2006). A novel role for Xist RNA in the formation of a repressive nuclear compartment into which genes are recruited when silenced.. Genes Dev. 20: 2223-2237 [Abstract] [Full Text]  
• Valley, C. M., Pertz, L. M., Balakumaran, B. S., Willard, H. F. (2006). Chromosome-wide, allele-specific analysis of the histone code on the human X chromosome. Hum Mol Genet 15: 2335-2347 [Abstract] [Full Text]  
• Luciani, J. J., Depetris, D., Usson, Y., Metzler-Guillemain, C., Mignon-Ravix, C., Mitchell, M. J., Megarbane, A., Sarda, P., Sirma, H., Moncla, A., Feunteun, J., Mattei, M.-G. (2006). PML nuclear bodies are highly organised DNA-protein structures with a function in heterochromatin remodelling at the G2 phase. J. Cell Sci. 119: 2518-2531 [Abstract] [Full Text]  
• Doyen, C.-M., An, W., Angelov, D., Bondarenko, V., Mietton, F., Studitsky, V. M., Hamiche, A., Roeder, R. G., Bouvet, P., Dimitrov, S. (2006). Mechanism of Polymerase II Transcription Repression by the Histone Variant macroH2A. Mol. Cell. Biol. 26: 1156-1164 [Abstract] [Full Text]  
• MASUI, O., HEARD, E. (2006). RNA and Protein Actors in X-Chromosome Inactivation. Cold Spring Harb Symp Quant Biol 71: 419-428 [Abstract]  
• Abbott, D. W., Chadwick, B. P., Thambirajah, A. A., Ausio, J. (2005). Beyond the Xi: MacroH2A CHROMATIN DISTRIBUTION AND POST-TRANSLATIONAL MODIFICATION IN AN AVIAN SYSTEM. J. Biol. Chem. 280: 16437-16445 [Abstract] [Full Text]  
• Kamakaka, R. T., Biggins, S. (2005). Histone variants: deviants?. Genes Dev. 19: 295-316 [Abstract] [Full Text]  
• Grigoryev, S. A., Nikitina, T., Pehrson, J. R., Singh, P. B., Woodcock, C. L. (2004). Dynamic relocation of epigenetic chromatin markers reveals an active role of constitutive heterochromatin in the transition from proliferation to quiescence. J. Cell Sci. 117: 6153-6162 [Abstract] [Full Text]  
• Hoyer-Fender, S., Czirr, E., Radde, R., Turner, J. M. A., Mahadevaiah, S. K., Pehrson, J. R., Burgoyne, P. S. (2004). Localisation of histone macroH2A1.2 to the XY-body is not a response to the presence of asynapsed chromosome axes. J. Cell Sci. 117: 189-198 [Abstract] [Full Text]  
• Turner, J. M. A., Mahadevaiah, S. K., Elliott, D. J., Garchon, H.-J., Pehrson, J. R., Jaenisch, R., Burgoyne, P. S. (2002). Meiotic sex chromosome inactivation in male mice with targeted disruptions of Xist. J. Cell Sci. 115: 4097-4105 [Abstract] [Full Text]  
• Chadwick, B. P., Willard, H. F. (2002). Cell cycle-dependent localization of macroH2A in chromatin of the inactive X chromosome. JCB 157: 1113-1123 [Abstract] [Full Text]  
• Maxfield Boumil, R., Lee, J. T. (2001). Forty years of decoding the silence in X-chromosome inactivation. Hum Mol Genet 10: 2225-2232 [Abstract] [Full Text]  
• Beletskii, A., Hong, Y.-K., Pehrson, J., Egholm, M., Strauss, W. M. (2001). PNA interference mapping demonstrates functional domains in the noncoding RNA Xist. Proc. Natl. Acad. Sci. USA 98: 9215-9220 [Abstract] [Full Text]  
• Chadwick, B. P., Valley, C. M., Willard, H. F. (2001). Histone variant macroH2A contains two distinct macrochromatin domains capable of directing macroH2A to the inactive X chromosome. Nucleic Acids Res 29: 2699-2705 [Abstract] [Full Text]  
• Chadwick, B. P., Willard, H. F. (2001). Histone H2A variants and the inactive X chromosome: identification of a second macroH2A variant. Hum Mol Genet 10: 1101-1113 [Abstract] [Full Text]  
• Chadwick, B. P., Willard, H. F. (2001). A Novel Chromatin Protein, Distantly Related to Histone H2a, Is Largely Excluded from the Inactive X Chromosome. JCB 152: 375-384 [Abstract] [Full Text]  
• Rasmussen, T. P., Mastrangelo, M.-A., Eden, A., Pehrson, J. R., Jaenisch, R. (2000). Dynamic Relocalization of Histone Macroh2a1 from Centrosomes to Inactive X Chromosomes during X Inactivation. JCB 150: 1189-1198 [Abstract] [Full Text]  
• Phillips, R. L., Ernst, R. E., Brunk, B., Ivanova, N., Mahan, M. A., Deanehan, J. K., Moore, K. A., Overton, G. C., Lemischka, I. R. (2000). The Genetic Program of Hematopoietic Stem Cells. Science 288: 1635-1640 [Abstract] [Full Text]  
• Costanzi, C, Stein, P, Worrad, D., Schultz, R., Pehrson, J. (2000). Histone macroH2A1 is concentrated in the inactive X chromosome of female preimplantation mouse embryos. Development 127: 2283-2289 [Abstract]  
• Gilbert, S. L., Pehrson, J. R., Sharp, P. A. (2000). XIST RNA Associates with Specific Regions of the Inactive X Chromatin. J. Biol. Chem. 275: 36491-36494 [Abstract] [Full Text]  
• Costanzi, C., Pehrson, J. R. (2001). MACROH2A2, a New Member of the MACROH2A Core Histone Family. J. Biol. Chem. 276: 21776-21784 [Abstract] [Full Text]  
• Hong, B., Reeves, P., Panning, B., Swanson, M. S., Yang, T. P. (2001). Identification of an autoimmune serum containing antibodies against the Barr body. Proc. Natl. Acad. Sci. USA 98: 8703-8708 [Abstract] [Full Text]  
• Grigoryev, S. A., Nikitina, T., Pehrson, J. R., Singh, P. B., Woodcock, C. L. (2004). Dynamic relocation of epigenetic chromatin markers reveals an active role of constitutive heterochromatin in the transition from proliferation to quiescence. J. Cell Sci. 117: 6153-6162 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents