An Actin-Binding Protein of the Sla2/Huntingtin Interacting Protein 1 Family Is a Novel Component of Clathrin-Coated Pits and Vesicles

doi:10.1083/jcb.147.7.1503

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© The Rockefeller University Press, 0021-9525/1999//1503 $5.00
The Journal of Cell Biology, Volume 147, Number 7, , 1999 1503-1518

Original Article

An Actin-Binding Protein of the Sla2/Huntingtin Interacting Protein 1 Family Is a Novel Component of Clathrin-Coated Pits and Vesicles

Åsa E.Y. Engqvist-Goldstein^a, Michael M. Kessels^a, Vikramjit S. Chopra^b, Michael R. Hayden^b, and David G. Drubin^a

^a Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720-3202
^b Centre for Molecular Medicine and Therapeutics, Department of Medical Genetics, Children's and Women's Hospital, University of British Columbia, Vancouver, British Columbia, Canada V5Z 4H4
401 Barker Hall, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3202.(510) 642-6420(510) 642-3692

drubin{at}uclink4.berkeley.edu

The actin cytoskeleton has been implicated in endocytosis, yetfew molecules that link these systems have been identified.Here, we have cloned and characterized mHip1R, a protein thatis closely related to huntingtin interacting protein 1 (Hip1).These two proteins are mammalian homologues of Sla2p, an actinbinding protein important for actin organization and endocytosisin yeast. Sequence alignments and secondary structure predictionsverified that mHip1R belongs to the Sla2 protein family. Thus,mHip1R contains an NH₂-terminal domain homologous to that implicatedin Sla2p's endocytic function, three predicted coiled–coils,a leucine zipper, and a talin-like actin-binding domain at theCOOH terminus. The talin-like domain of mHip1R binds to F-actinin vitro and colocalizes with F-actin in vivo, indicating thatthis activity has been conserved from yeast to mammals. mHip1Rshows a punctate immunolocalization and is enriched at the cellcortex and in the perinuclear region. We concluded that thecortical localization represents endocytic compartments, becausemHip1R colocalizes with clathrin, AP-2, and endocytosed transferrin,and because mHip1R fractionates biochemically with clathrin-coatedvesicles. Time-lapse video microscopy of mHip1R–greenfluorescence protein (GFP) revealed a blinking behavior similarto that reported for GFP-clathrin, and an actin-dependent inwardmovement of punctate structures from the cell periphery. Thesedata show that mHip1R is a component of clathrin-coated pitsand vesicles and suggest that it might link the endocytic machineryto the actin cytoskeleton.

Key Words: actin • endocytosis • clathrin • Huntington disease • vesicle

Abbreviations used in this paper: aa, amino acid(s); EST, expressedsequence tag; GFP, green fluorescence protein; GST, glutathioneS-transferase; Hip1, huntingtin interacting protein 1; LAT-A,latrunculin-A.

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