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© The Rockefeller University Press, 0021-9525/2000//127 $5.00
The Journal of Cell Biology, Volume 148, Number 1, , 2000 127-136

Original Article

Cell Spreading and Lamellipodial Extension Rate Is Regulated by Membrane Tension



Drazen Rauchera and Michael P. Sheetza

a Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710
Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, NC 27710.(919) 684-8592(919) 684-8091

m.sheetz{at}cellbio.duke.edu

Cell spreading and motility require the extension of the plasma membrane in association with the assembly of actin. In vitro, extension must overcome resistance from tension within the plasma membrane. We report here that the addition of either amphiphilic compounds or fluorescent lipids that expanded the plasma membrane increased the rate of cell spreading and lamellipodial extension, stimulated new lamellipodial extensions, and caused a decrease in the apparent membrane tension. Further, in PDGF-stimulated motility, the increase in the lamellipodial extension rate was associated with a decrease in the apparent membrane tension and decreased membrane–cytoskeleton adhesion through phosphatidylinositol diphosphate hydrolysis. Conversely, when membrane tension was increased by osmotically swelling cells, the extension rate decreased. Therefore, we suggest that the lamellipodial extension process can be activated by a physical signal (perhaps secondarily), and the rate of extension is directly dependent upon the tension in the plasma membrane. Quantitative analysis shows that the lamellipodial extension rate is inversely correlated with the apparent membrane tension. These studies describe a physical chemical mechanism involving changes in membrane–cytoskeleton adhesion through phosphatidylinositol 4,5-biphosphate–protein interactions for modulating and stimulating the biochemical processes that power lamellipodial extension.

Key Words: membrane–cytoskeleton adhesion • membrane expansion • laser optical tweezers • cell spreading • membrane tether

© 2000 The Rockefeller University Press

Abbreviations used in this paper: β-DPH-HPC, 2-(3-(diphenylhexatrienyl)propanoyl)-1-hexadecanoyl-sn-glycero-3-phosphocholine; C5-DMB-SM, N-(4,4-difluoro-5,7-dimethyl-4-bora-3{alpha},4{alpha}-diaza-indacene-3-pentanoyl) sphingosyl phosphocholine (BODIPY FL C5-sphyngomyelin); DiA, 4-4(4-(didecylamino)styryl)-N-methyl-pyridiniumiodide (4-Di-10-ASP); DIC, differential interference contrast; FITC-PL; L-{alpha}-phosphatidylethanolamine, dipalmitoyl, N-(5-fluoresceinthiocarbamoyl); PIP2, phosphatidylinositol 4,5-biphosphate.


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